Mr. Davide Volpi Profile

Davide Volpi

PhD Student

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Area of Expertise:

Biophotonics , Image Processing , Fluorescence Imaging , Imaging , Light Transport Modelling

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Proceedings Article | 27 February 2014 Paper

A novel multiwavelength fluorescence image-guided surgery imaging system

D. Volpi, I. Tullis, A. Laios, P. N. Pathiraja, K. Haldar, A. Ahmed, B. Vojnovic

Proceedings Volume 8935, 89350G (2014) https://doi.org/10.1117/12.2038377

KEYWORDS: Laparoscopy, Imaging systems, Luminescence, Surgery, Lymphatic system, Cancer, Cameras, Near infrared, Reflectivity, Image-guided intervention

Read Abstract +

We describe the development and performance analysis of two clinical near-infrared fluorescence image-guided surgery (FIGS) devices that aim to overcome some of the limitations of current FIGS systems. The devices operate in a widefield-imaging mode and can work (1) in conjunction with a laparoscope, during minimally invasive surgery, and (2) as a hand-held, open surgery imaging system. In both cases, narrow-band excitation light, delivered at multiple wavelengths, is efficiently combined with white reflectance light. Light is delivered to ~100 cm² surgical field at 1-2 mW/cm² for white light and 3-7 mW/cm² (depending on wavelength) of red - near infrared excitation, at a typical working distance of 350 mm for the hand-held device and 100 mm for the laparoscope. A single, sensitive, miniaturized color camera collects both fluorescence and white reflectance light. The use of a single imager eliminates image alignment and software overlay complexity. A novel filtering and illumination arrangement allows simultaneous detection of white reflectance and fluorescence emission from multiple dyes in real-time. We will present both fluorescence detection sensitivity modeling and practical performance data. We have demonstrated the efficiency and the advantages of the devices both pre-clinically and during live surgery on humans. Both the hand-held and the laparoscopic systems have proved to be reliable and beneficial in an ongoing clinical trial involving sentinel lymph node detection in gynecological cancers. We will show preliminary results using two clinically approved dyes, Methylene blue and indocyanine green. We anticipate that this technology can be integrated and routinely used in a larger variety of surgical procedures.

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