Amplitude modulation of backscattered light caused by blood volume fluctuations was studied in Laser Doppler Flowmetry. It was shown that the amplitude-modulated signal significantly contributes to the power spectral density of photocurrent and calculated perfusion.
The widespread introduction of laser noninvasive diagnostic techniques in medicine gave rise interest to theoretical description of light propagation in turbid media. One of the purposes for that is a preliminary simulation of incoming radiation for diagnostic spectrophotometry equipment. For complex diagnostic devices combining the Laser Doppler Flowmetry (LDF) and the tissue reflectance oximetry (TRO) it is necessary to know a ratio of signals in each diagnostic channel for a proper choice of the radiation power of laser sources, sensitivity of photodetectors, etc. In LDF the lightbeating backscattered signal mixed from moving red blood cells and static inhomogeneities inside the tissue is the useful signal, while in TRO both signals from static and moving scatterers are registered in the sum. The aim of our study was an estimation of the ratio between flux with the Doppler shifted signal and the total backscattered flux. For this purpose the simple analytical model describing the backscattered radiation for a two-layered tissue with different levels of blood volume in the second layer was under consideration. The physical model was based on the improved Kubelka-Munk approach. This approach involves an additional parameter of the density of scatterers, so it is useful for the Doppler signal intensity calculation as well. To assess the intensity of the Doppler component the single-scattering approximation inside the tissue’s second layer was used. It was found that the fraction of the Doppler component in the total backscattered flux can vary in the range of 1-10% for the blood volume of 1-20%.