In the light of the characteristics of pedestrian-induced vibration comfort evaluation of long-span footbridges, a real-time evaluation system of pedestrian-induced footbridge vibration comfort was developed based on Android platform for the first time. The system is composed of data acquisition subsystem, management center subsystem and Android mobile client. On the basis of collecting pedestrian-induced vibration signals by using the built-in high-precision acceleration sensor, space coordinate transformation algorithm was used to the dynamic signal acquisition process, transformation from smartphone’s coordinate system to the inertial coordinate system was achieved. Wavelet transformation was used to isolate gravity from the raw signals, the aim of this paper is to develop a real-time evaluation system. The system was tested on Guangzhou Gangding Footbridge, the field test results show that the comfort degree obtained from the system was identical with the truth, which demonstrates that the real-time vibration comfort evaluation system is scientific and effective, it makes up for the vacancy of real-time evaluation of pedestrian-induced footbridge vibration comfort.