Ms. Diana Bradbury Profile

Diana Bradbury

at Flinders Univ

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Proceedings Article | 30 December 2008 Paper

Magnetic and fluorescence-encoded polystyrene microparticles for cell separation

Diana Bradbury, Emily Anglin, Sheree Bailey, Peter Macardle, Michael Fenech, Helmut Thissen, Nicolas Voelcker

Proceedings Volume 7267, 726711 (2008) https://doi.org/10.1117/12.812208

KEYWORDS: Particles, Proteins, Magnetism, Luminescence, Nanoparticles, Tissues, Iron, Oxides, Microscopy, Quantum dots

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Materials assisting with the efforts of cell isolation are attractive for numerous biomedical applications including tissue engineering and cell therapy. Here, we have developed surface modification methods on microparticles for the purposes of advanced cell separation. Iron oxide nanoparticles were incorporated into 200 ìm polystyrene microparticles for separation of particle-bound cells from non-bound cells in suspension by means of a permanent magnet. The polystyrene microparticles were further encoded with fluorescent quantum dots (QD) as identification tags to distinguish between specific microparticles in a mixture. Cluster of differentiation (CD) antibodies were displayed on the surface of the microparticles through direct adsorption and various methods of covalent attachment. In addition, a protein A coating was used to orientate the antibodies on the microparticle surface and to maximise accessibility of the antigen-binding sites. Microparticles which carried CD antibodies via covalent attachment showed greater cell attachment over those modifications that were only adsorbed to the surface through weak electrostatic interactions. Greatest extent of cell attachment was observed on microparticles modified with protein A - CD antibody conjugates. B and T lymphocytes were successfully isolated from a mixed population using two types of microparticles displaying B and T cell specific CD antibodies, respectively. Our approach will find application in preparative cell separation from tissue isolates and for microcarrier-based cell expansion.

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