Paper performs results of SERS-active surfaces fabrication for Raman bacterial cells analysis. Based on FDTD simulation, the synthesis of colloidal gold nanoparticles (NPs) with the size range of 10 – 100 nm has been performed by the following methods: a) femtosecond laser ablation of a plate in a liquid; b) chemical reduction from tetrachloroauric acid trihydrate (HAuCl4). Optimal sizes and shapes of the particles with a maximum of plasmon absorption in the range 500 – 800 nm have been determined by numerical simulation. For NPs deposited on quartz glass with rodamine 6G (R6G) and E. Coli bacterial cells, SERS solutions have been tested.
This paper perform the results of studies on the development of a simple methodology for creating hydrosol-modified silver and gold nanoparticles with a size of 44 nm (silver) and 54 nm (gold) quartz surfaces for sensory purposes. Proposed surfaces is able to perform the effect of Surface-enhanced Raman scattering (SERS) of light by dye molecules of Rhodamine 6G. It was shown that the order of amplification of the Raman signal by surface plasmons of silver and gold nanoparticles for Rhodamine 6G dye molecules can reach orders of magnitude 102 times. A method for the controlled synthesis of silver hydrosols by methods of reducing sodium salt AgNO3 with sodium citrate and femtosecond laser ablation for gold is proposed. The prospects of such systems for obtaining spectra of the molecular structure of dyes are shown. The results of this work, in the future, can be used to analyze chemical compounds of low concentrations, macroscopic biological objects like bacterial cells and blood components.