Brillouin spectroscopy has recently emerged as a valuable tool for assessing microscopic viscoelastic properties in biological tissues and cells. For many practical biomedical applications, the viscoelastic measurement techniques should be sensitive to low sample concentrations in biological media. In this report, we assess the sensitivity of a recently improved impulsive stimulated Brillouin scattering (ISBS) setup. We explored biologically relevant solutions in distilled water using citric acid, glycine, and sucrose, for which we performed Brillouin measurements. We detailed the peak fitting methodology and analyzed the Brillouin shift and linewidth as a function of concentration. We discuss the sensitivity of the ISBS setup to low concentration measurements and its implications to biological applications.
Dysfunctions in the endothelial cell lining of the vascular endothelium are linked with human pathogenesis of atherosclerosis, venous thrombosis, and several human viral infections. These diseases typically originate from abnormalities resulting from poor structural integrity of the tunica intima of the vascular endothelium. In this report, impulsive stimulated Brillouin scattering spectroscopy was used to assess viscoelastic properties of cells in a microfluidic chip which was designed to mimic the vascular endothelium tunica intima. Brillouin spectroscopy method enabled non-invasive data acquisition of viscoelastic measurements to understand the role of collagen type I on the anchoring of endothelial cells to the extracellular matrix.
Brillouin microscopy is an emerging imaging modality in a broad area of biomedical research and clinical applications. Over the past decade, a significance progress has been achieved in developing better, more accurate and more user-friendly instrumentation for Brillouin microscopy and in fundamental understanding of the imaging contrast affordable in Brillouin microscopy. In this report, we report on our progress on developing advanced Brillouin microscopy imaging for imaging of dynamic biological processes.