Dr. Donna J. Arndt-Jovin Profile

Dr. Donna J. Arndt-Jovin

at Max-Planck-Institut für Biophysikalische Chemie

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Publications (6)

Proceedings Article | 10 March 2015 Paper

Generation 3 programmable array microscope (PAM) for high speed large format optical sectioning in fluorescence

Anthony de Vries, Nathan Cook, Stephan Kramer, Donna Arndt-Jovin, Thomas Jovin

Proceedings Volume 9376, 93760C (2015) https://doi.org/10.1117/12.2076390

KEYWORDS: Digital micromirror devices, Cameras, Microscopes, Luminescence, Diffraction, Optical filters, Optical lithography, Signal to noise ratio, Receptors, Light sources

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We report on the current version of the optical sectioning programmable array microscope (PAM) implemented with a single digital micro-mirror device (DMD) spatial light modulator utilized as a mask in both the fluorescence excitation and emission paths. The PAM incorporates structured illumination and structured detection operating in synchrony. A sequence of binary patterns of excitation light in high definition format (1920×1080 elements) is projected into the focal plane of the microscope at the 18 kHz binary frame rate of the Texas Instruments 1080p DMD. The resulting fluorescent emission is captured as two distinct signals: conjugate (c, ca. “on-focus”) consisting of light impinging on and deviated from the “on” elements of the DMD, and the non-conjugate (nc, ca. “out-of-focus”) light falling on and deviated from the “off” elements. The two distinct, deflected beams are optically filtered and detected either by two individual cameras or captured as adjacent images on a single camera after traversing an image combiner. The sectioned image is gained from a subtraction of the nc image from the c image, weighted in accordance with the pattern(s) used for illumination and detection and the relative exposure times of the cameras. The widefield image is given by the sum of the c and nc images. This procedure allows a high duty cycle (typically 25-50%) of on-elements in the excitation patterns and thus functions with low light intensities, preventing saturation and minimizing photobleaching of sensitive fluorophores. The corresponding acquisition speed is also very high, limited only by the bandwidth of the camera(s) (100 fps full frame with the sCMOS camera in current use) and the optical power of the light source (lasers, large area LEDs). In contrast to the static patterns typical of SIM systems, the programmable array allows optimization of the patterns (duty cycle, feature size and distribution), thus enabling a wide range of applications, ranging from patterned photobleaching, (e.g., FRAP, FLIP) and photoactivation, spatial superresolution, automated adaptive tracking and minimization of light exposure (MLE), and photolithography.

Proceedings Article | 15 February 2011 Paper

Generation-3 programmable array microscope (PAM) with digital micro-mirror device (DMD)

Pieter A. A. De Beule, Anthony H. B. de Vries, Donna Arndt-Jovin, Thomas Jovin

Proceedings Volume 7932, 79320G (2011) https://doi.org/10.1117/12.879611

KEYWORDS: Digital micromirror devices, Luminescence, Microscopes, Diffraction, Imaging systems, Glasses, Optical design, Spatial light modulators, Mirrors, Receptors

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Proceedings Article | 31 March 2007 Paper

Biological applications of an LCoS-based programmable array microscope (PAM)

Guy Hagen, Wouter Caarls, Martin Thomas, Andrew Hill, Keith Lidke, Bernd Rieger, Cornelia Fritsch, Bert van Geest, Thomas Jovin, Donna Arndt-Jovin

Proceedings Volume 6441, 64410S (2007) https://doi.org/10.1117/12.710995

KEYWORDS: Microscopes, Luminescence, Spatial light modulators, Fluorescence lifetime imaging, Receptors, Image processing, Liquid crystal on silicon, Molecules, Confocal microscopy, Diffusion

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Proceedings Article | 27 March 2006 Paper

In vivo cell imaging with semiconductor quantum dots and noble metal nanodots

Donna Arndt-Jovin, M. Arturo Lopez-Quintela, Diane Lidke, María Rodríguez, Francisco Martinez Santos, Keith Lidke, Guy Hagen, Thomas Jovin

Proceedings Volume 6096, 60960P (2006) https://doi.org/10.1117/12.646794

KEYWORDS: Gold, Receptors, Quantum dots, Semiconductors, Luminescence, Particles, In vivo imaging, Proteins, Metals, Electrodes

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Proceedings Article | 1 December 1991 Paper

Digital imaging microscopy: the marriage of spectroscopy and the solid state CCD camera

Thomas Jovin, Donna Arndt-Jovin

Proceedings Volume 1439, (1991) https://doi.org/10.1117/12.50457

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Proceedings Article | 1 June 1991 Paper

Rotational diffusion of receptors for epidermal growth factor measured by time-resolved phosphorescence depolarization

Raphael Zidovetzki, David Johnson, Donna Arndt-Jovin, Thomas Jovin

Proceedings Volume 1432, (1991) https://doi.org/10.1117/12.44211

KEYWORDS: Receptors, Phosphorescence, Diffusion, Picosecond phenomena, Proteins, Fluorescence anisotropy, Tissues, Anisotropy, Spectroscopy, Optical filters

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