Dr. Ekaterina Lukianova Profile

Dr. Ekaterina Lukianova

at AV Luikov Heat and Mass Transfer Institute

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Author

Publications (9)

Proceedings Article | 28 February 2008 Paper

Photothermolysis by laser-induced microbubbles generated around gold nanorod clusters selectively formed in leukemia cells

Dmitri Lapotko, Ekaterina Lukianova-Hleb, Sergei Zhdanok, Betty Rostro, Rebecca Simonette, Jason Hafner, Marina Konopleva, Michael Andreeff, Andre Conjusteau, Alexander Oraevsky

Proceedings Volume 6856, 68560K (2008) https://doi.org/10.1117/12.771660

KEYWORDS: Tumors, Gold, Scattering, Pulsed laser operation, Laser scattering, Nanoparticles, Light scattering, Absorption, Leukemia, Nanorods

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Proceedings Article | 7 August 2007 Paper

LANTCET: laser nanotechnology for screening and treating tumors ex vivo and in vivo

Dmitri Lapotko, Ekaterina Lukianova-Hleb, Sergei Zhdanok, Jason Hafner, Betty Rostro, Peter Scully, Marina Konopleva, Michael Andreeff, Chun Li, Ehab Hanna, Jeffrey Myers, Alexander Oraevsky

Proceedings Volume 6735, 673516 (2007) https://doi.org/10.1117/12.753356

KEYWORDS: Tumors, Pulsed laser operation, Gold, Scattering, Light scattering, Nanoparticles, Laser damage threshold, Laser scattering, In vivo imaging, Nanorods

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LANTCET (laser-activated nano-thermolysis as cell elimination technology) was developed for selective detection and destruction of individual tumor cells through generation of photothermal bubbles around clusters of light absorbing gold nanoparticles (nanorods and nanoshells) that are selectively formed in target tumor cells. We have applied bare nanoparticles and their conjugates with cell-specific vectors such as monoclonal antibodies CD33 (specific for Acute Myeloid Leukemia) and C225 (specific for carcinoma cells that express epidermal growth factor -EGF). Clusters were formed by using vector-receptor interactions with further clusterization of nanoparticles due to endocytosis. Formation of clusters was verified directly with optical resonance scattering microscopy and microspectroscopy. LANTCET method was tested in vitro for living cell samples with: (1) model myeloid K562 cells (CD33 positive), (2) primary human bone marrow CD33-positive blast cells from patients with the diagnosis of acute myeloid leukemia, (3) monolayers of living EGF-positive carcinoma cells (Hep-2C), (4) human lymphocytes and red blood cells as normal cells. The LANTCET method was also tested in vivo using rats with experimental polymorphic sarcoma. Photothermal bubbles were generated and detected in vitro with a photothermal microscope equipped with a tunable Ti-Sa pulsed laser. We have found that cluster formation caused an almost 100-fold decrease in the bubble generation threshold of laser pulse fluence in tumor cells compared to the bubble generation threshold for normal cells. The animal tumor that was treated with a single laser pulse showed a necrotic area of diameter close to the pump laser beam diameter and a depth of 1-2 mm. Cell level selectivity of tumor damage with single laser pulse was demonstrated. Combining lightscattering imaging with bubble imaging, we introduced a new image-guided mode of the LANTCET operation for screening and treatment of tumors ex vivo and in vivo.

Proceedings Article | 1 August 2007 Paper

Nanocluster: photothermal bubble as optical probes for cytometric and microscopic applications

Dmitri Lapotko, Ekaterina Lukianova-Hleb, Jason Hafner

Proceedings Volume 6734, 67340E (2007) https://doi.org/10.1117/12.753159

KEYWORDS: Scattering, Nanoparticles, Light scattering, Target detection, Signal detection, Molecules, Luminescence, Gold, Microscopy, Nanorods

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The ability to detect optical signals form a cellular target depends upon the amount of optical energy that can be generated by this target as the signal. Given that the sensitivity of optical detectors has some finite limit, further increase of the sensitivity of optical diagnostic methods requires increasing the energy of target-generated signal. Usually this energy is converted by the cellular target upon its optical excitation and is limited by many factors such as: cell and target damage thresholds, efficiency of excitation energy conversion, size of the target etc. All these put principal limitation on sensing small targets (like molecules) in living cells with any optical method because the energy that can be safely converted by the target into a signal is limited. To overcome this limitation and to improve the sensitivity of optical microscopy of living cells (and cytometry in general) we propose the concept of intracellular amplification of the optical signal. This concept includes two major steps. First, primary (pump) optical radiation interacts with the target (a probe molecule) to generate a transient target. Second, the transient target is sensed with additional optical radiation that does not interact strongly with primary target or the cell, and, hence, may have high enough energy to increase the signal from transient target even above the energy of pump radiation, which is limited by cell and target damage thresholds. We propose to use optical scattering from clusters of gold nanoparticles (the target) that are selectively formed in specific cells through antibody-receptor interaction and through endocytosis. To amplify this optical signal we propose to generate photothermal bubbles (the transient target) around those clusters. In experiments with water suspensions and with individual tumor K562 cells we have achieved optical signal amplification in individual cells (relatively to the scattering signal from intact cells): with gold nanorod intracellular clusters, 14.8 times, with photothermal bubbles, generated around those clusters, more than 100 times. Those signals were much higher than corresponding fluorescent signals and were obtained from living cells.

Proceedings Article | 26 February 2007 Paper

Photothermal and photoacoustic processes of laser activated nano-thermolysis of cells

Dmitri Lapotko, Ekaterina Lukianova, Pavel Mitskevich, Victoria Smolnikova, Michail Potapnev, Marina Konopleva, Michael Andreeff, Alexander Oraevsky

Proceedings Volume 6437, 64370C (2007) https://doi.org/10.1117/12.713082

KEYWORDS: Tumors, Pulsed laser operation, Nanoparticles, Neptunium, Gold, Leukemia, Laser damage threshold, Safety, Laser safety, Bone

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Proceedings Article | 10 February 2007 Paper

Methods for monitoring and imaging nanoparticles in cells

Dmitri Lapotko, Ekaterina Lukianova, Sergey Chizhik

Proceedings Volume 6447, 644703 (2007) https://doi.org/10.1117/12.712855

KEYWORDS: Neptunium, Nanoparticles, Gold, Microscopy, Scanning electron microscopy, Light scattering, Fermium, Frequency modulation, Signal detection, Pulsed laser operation

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Proceedings Article | 6 March 2006 Paper

Elimination of leukemic cells from human transplants by laser nano-thermolysis

Dmitri Lapotko, Ekaterina Lukianova, Michail Potapnev, Olga Aleinikova, Alexander Oraevsky

Proceedings Volume 6086, 60860I (2006) https://doi.org/10.1117/12.658139

KEYWORDS: Tumors, Nanoparticles, Bone, Pulsed laser operation, Laser safety, Leukemia, Laser therapeutics, Gold, Safety, Cancer

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Proceedings Article | 21 February 2006 Paper

Laser-activated bubbles in living cells: new universal cytometric method

Dmitri Lapotko, Ekaterina Lukianova

Proceedings Volume 6088, 608818 (2006) https://doi.org/10.1117/12.658160

KEYWORDS: Pulsed laser operation, Nanoparticles, Microscopy, Blood, Tumors, Signal detection, Laser beam diagnostics, Absorption, Laser damage threshold, Flow cytometry

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Proceedings Article | 25 April 2005 Paper

Laser activated nanothermolysis of leukemia cells monitored by photothermal microscopy

Dmitri Lapotko, Ekaterina Lukianova, Alexander Shnip, George Zheltov, Michail Potapnev, Valeriy Savitsky, Olga Klimovich, Alexander Oraevsky

Proceedings Volume 5697, (2005) https://doi.org/10.1117/12.596372

KEYWORDS: Leukemia, Nanoparticles, Gold, Blood, Pulsed laser operation, Laser damage threshold, Microscopy, Tumors, Laser therapeutics, Laser beam diagnostics

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SPIE Journal Paper | 1 January 2005 Open Access

Photothermal responses of individual cells

Dmitri Lapotko, Alexander Shnip, Ekaterina Luk'yanova

JBO, Vol. 10, Issue 01, 014006, (January 2005) https://doi.org/10.1117/12.10.1117/1.1854685

KEYWORDS: Pulsed laser operation, Laser beam diagnostics, Absorption, Thermography, Signal detection, Blood, Tumors, Temperature metrology, Microscopes, Laser damage threshold

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