Proc. SPIE. 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX
KEYWORDS: 3D acquisition, Image processing, Microscopy, Luminescence, Image acquisition, Fluorescence lifetime imaging, Physical phenomena, Testing and analysis, 3D image processing, Current controlled current source
We compare various time-gated and discrete-time methods for extracting the decay constant from an exponential signal, typical of fluorescence lifetime (FLIM) instruments, as well as a host of other physical phenomena. Analytical methods are evaluated for accuracy and precision as well as for computational and sampling efficiency.