We introduce stimuli-responsive structures fabricated by 3D laser lithography. The key material is a responsive hydrogel. It is based on supramolecular host-guest chemistry, which allows reversible actuation under physiological conditions. Combined with other photoresists, multi-material scaffolds are used to directly study live cell behavior during spatially and temporally well-defined changes in the extracellular surrounding. Digital image correlation enables us to precisely track and analyze the behavior of a multitude of cells in a single experiment. With this versatile technique we will study the mechanoresponse of cells and the role of different proteins in the mechanotransduction.