Nonlinear optical microcopy has become an important tool in investigating biomaterials due to its various advantages such as label-free imaging capabilities. In particular, it has been shown that third-harmonic generation (THG) signals can be produced at interfaces between an aqueous medium (e.g. cytoplasm, interstitial fluid) and a mineralized lipidic surface. In this work, we have demonstrated that label-free high-contrast THG images of the mycobacterium tuberculosis can be obtained using THG microscopy.
Proc. SPIE. 7214, Ultrafast Phenomena in Semiconductors and Nanostructure Materials XIII
KEYWORDS: Ferroelectric materials, Quantum wells, Luminescence, Gallium arsenide, Single photon, Near field scanning optical microscopy, Near field, Tapered optical fibers, Temperature metrology, Absorption
We demonstrate an experimental setup combining the near-field scanning optical microscope (NSOM) and timecorrelated
single photon counting (TCSPC) system for high-resolution temporal and spatial spectroscopic measurements.
In particular, a multiple-quantum-well (MQW) structure was excited with a tapered optical fiber with 100 nm opening
aperture in near-field region and their temporal photoluminescence spectra were obtained by TCSPC system. We are
able to measure fluorescence decay time of a GaAs/AlGaAs MQW structure with well width of 75Å in the near-field
region at room temperature.