Accurate and traceable reference coordinates in three-dimensional space is the key and difficult point for coordinate calibration of large-scale measurement instruments such as laser tracker and iGPS. This paper studies the application of multilateration with laser tracker in establishing reference coordinates. First, a reference coordinate network is established, which has good spatial scalability and is compatible with multiple targets. Then, multilateration with laser tracker is applied to calibrate the reference coordinate network. And the basic principle, measurement uncertainty evaluation and tracker layout optimization are studied in detail. So that the reference coordinates are traced to the laser interference. Finally, through the repeatability test, length test, and coordinate test, it is shown that the reference coordinates satisfy their measurement uncertainty range and can be used for coordinate calibration of the large-scale measurement instruments.