Sentinel lymph node biopsy is the gold standard to detect metastatic invasion from primary breast cancer. This method
can help patients avoid full axillary chain dissection, thereby decreasing the risk of morbidity. We propose an alternative
to the traditional isotopic method, to detect and map the sentinel lymph nodes. Indeed, Patent Blue V is the most widely
used dye in clinical routine for the visual detection of sentinel lymph nodes. A Recent study has shown the possibility of
increasing the fluorescence quantum yield of Patent Blue V, when it is bound to human serum albumin. In this study we
present a preclinical fluorescence imaging system to detect sentinel lymph nodes labeled with this fluorescent tracer. The
setup is composed of a black and white CCD camera and two laser sources. One excitation source with a laser emitting at
635 nm and a second laser at 785 nm to illuminate the region of interest. The prototype is operated via a laptop.
Preliminary experiments permitted to determine the device sensitivity in the μmol.L<sup>-1</sup> range as regards the detection of
PBV fluorescence signals. We also present a preclinical evaluation performed on Lewis rats, during which the
fluorescence imaging setup detected the accumulation and fixation of the fluorescent dye on different nodes through the
Sentinel lymph node biopsy is the gold standard method to detect a metastatic invasion from the primary breast cancer.
This method can avoid patients to be submitted to full axillary chain dissection. In this study we present and compare
two near-infrared optical probes for the sentinel lymph node detection, based on the recording of scattered photons. The
two setups were developed to improve the detection of the dye injected in clinical routine: the Patent Blue V dye. Herein,
we present results regarding clinical ex-vivo detection of sentinel lymph node after different volume injections. We have
previously published results obtained with a two-wavelength probe on phantom and animal models. However this first
generation device did not completely account for the optical absorption variations from biological tissue. Thus, a second
generation probe has been equipped with four wavelengths. The dye concentration computation is then more robust to
measurement and tissue property fluctuations. The detection threshold of the second setup was estimated at 8.10<sup>-3</sup>μmol/L, which is about 37 times lower than the eye visibility threshold. We present here the preliminary results and
demonstrate the advantages of using four wavelengths compared to two on phantom suspensions simulating the optical
properties of breast tissues.