A molecular imaging method employing acetic acid dilute solution as a biomarker of cervical neoplasia is described and
the interpretation of the biophysical processes involved determining the in-vivo measured dynamic scattering
characteristics is presented. A compartmental model of the epithelium has been developed for predicting the epithelial
transport phenomena that are expected to be correlated with the dynamic characteristics of the backscattered light.
Model predictions have been compared and confirmed by experimental data obtained from patients with cervical
neoplasia of different grade, with the aid of specially developed imaging system. Results revealed that the dynamic
scattering characteristics and both structural and functional alterations are largely determined by the intracellular proton
concentration kinetics. This highlights the potential of the developed imaging method and technology for the non-invasive
diagnosis, guided therapeutics and screening of cervical neoplasia.