Acousto-optical tunable filters (AOTFs) based on interaction of light and ultrasound in uniaxial birefringent crystals are widely used in imaging spectroscopy applications due to random spectral access, narrow controllable bandwidth, compactness, overal ease-of-use and image trasmittance capability. The spectral transmission of AOTF-based spectral imagers is usually characterized for paraxial light beam, but AOTFs inherently have non- uniform spatio-spectral transmission, so the central wavelength of the transmission window varies with the angle of incident light. We demonstrate that the spatio-spectral characteristics of acousto-optic (AO) interaction may be described either by the dependency of ultrasound frequency on the incident light angle for a given wavelength or by the dependency of wavelength on the incident angle for a given sound frequency. These dependencies are derived from the phase matching condition and are determined by the refractive indices, sound phase velocities and the AO diffraction geometry. We experimentally estimate the appearance of this specific spatio-spectral structure on the images acquired by AOTF-based imagers based on both collimating and confocal (telecentric) schemes and show that the variations of central wavelength and spectral bandwidth are noticeable for collimating setup even in the commonly used small field of view. The results of the study may be applied in design of AOTF- based spectral imagers and image processing algorithms.
In this study, we address the problem of automated optical trapping using optical tweezers. We demonstrate that using acousto-optical deflector allows implementing a wide variety of specific modes such as multi-trapping and sorting. Our experiments show that this approach enables fast and non-mechanical optical manipulation of microscopic particles according to pre-defined trajectories and tasks.
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