The use of fluorescence imaging of vascular permeability becomes a golden standard for assessing the inflammation process during experimental immune response in vivo. The use of the optical fluorescence imaging provides a very useful and simple tool to reach this purpose. The motivation comes from the necessity of a robust and simple quantification and data presentation of inflammation based on a vascular permeability. Changes of the fluorescent intensity, as a function of time is a widely accepted method to assess the vascular permeability during inflammation related to the immune response. In the present study we propose to bring a new dimension by applying a more sophisticated approach to the analysis of vascular reaction by using a quantitative analysis based on methods derived from astronomical observations, in particular by using a space-time Fourier filtering analysis followed by a polynomial orthogonal modes decomposition. We demonstrate that temporal evolution of the fluorescent intensity observed at certain pixels correlates quantitatively to the blood flow circulation at normal conditions. The approach allows to determine the regions of permeability and monitor both the fast kinetics related to the contrast material distribution in the circulatory system and slow kinetics associated with extravasation of the contrast material. Thus, we introduce a simple and convenient method for fast quantitative visualization of the leakage related to the inflammatory (immune) reaction in vivo.
SMESE (SMall Explorer For the study of Solar Eruptions) is a Franco-Chinese microsatellite mission. The scientific
objectives of SMESE are the study of coronal mass ejections and flares. Its payload consists of three instrument
packages : LYOT, DESIR and HEBS. LYOT is composed of a Lyman α (121.6 nm) coronagraph, a Lyman α disk imager and a far UV disk imager. DESIR is an infrared telescope working at 35 μm and 150 μm. HEBS is
a high energy burst spectrometer working in X rays and γ rays covering the 10 keV to 600 MeV range. SMESE
will be launched around 2011, providing a unique opportunity of detecting and understanding eruptions at the
maximum activity phase of the solar cycle in a wide range of energies. The instrumentation on board SMESE is
described in this paper.