Visible component of sunlight has a physiologically significant effect on human skin and long-term exposure to concentrated blue light energy (sunlight, laptop, cell phones) could produce oxidative stress leading to the premature skin aging. In this work, in vivo confocal Raman spectroscopy was used to characterize biochemical changes in human skin after been irradiated with different doses of blue light. After ethical committee approval, volunteers’ phototype I and II (Fitzpatrick classification) have been selected. The River Diagnosis confocal Raman spectrometer was used, before (T0) and after 15, 30 and 60 minutes of blue light irradiation (LED 450 nm) with doses of 100 J/cm2 . It was possible to evaluate the biochemical skin damage caused in the stratum corneum and in the viable epidermis.
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