In this work we introduce a theoretical model for light propagation in multilayered, turbid cylinders with an infinitely
thick bottom layer, which can be applied to the study of biological systems such as the human head. Our approach was
validated with experiments on a three-layered phantom and with Monte Carlo simulations. We show that the absorption
and the reduced scattering coefficient of the deepest layer can be retrieved within reasonable errors.
Diffuse optical imaging of the human brain requires methods to account for the layered structure of the head. In this work we present results of experiments performed on layered phantoms in reflection geometry by a time-resolved technique. We investigate structures with two and three layers with the goal to retrieve the optical properties of the deepest one. Data analysis is based on an existing solution of the time-resolved diffusion equation for a multilayer cylinder. Using a sufficiently large source-detector separation the absorption and reduced scattering coefficients of the deepest layer can be derived from time-resolved reflectance with a deviation of typically not more than 10% from the known values.