In this paper we present a newly developed digital transmission holographic microscope. The microscope enables using
arbitrarily low coherent illumination (both spatially and temporally) in conjunction with the off-axis holography. The
setup of the microscope, its function and the object wave reconstruction procedure are described. The optical sectioning
effect, similar to a confocal microscope, resulting from the use of low spatially coherent light source is demonstrated.
The microscope has been tailored for studies of living cell dynamics. Time-lapse phase reconstruction series of live cells
activities were carried out. The different behavior related to changes in the cell cycle is demonstrated.
The paper refers about the main characteristics of low-coherent holographic imaging in transmitted light, like optical
sectioning, optical path differences (OPD) measurement and high frame rate imaging. To demonstrate the optical
sectioning property of the microscope, we performed the measurement using laser as a coherent light source and
a halogen lamp with interference filter as a low-coherent light source. We observed the same place of the model sample
uncovered and then covered with a volume diffuser.