We propose a fast surface profiling measurement method using a color confocal microscope based on time-encoded spectroscopy. The chromatic confocal microscopy can acquire depth information at high speed because it does not require depth scanning. On the other hand, in chromatic confocal microscopy, depth information is obtained through the wavelength of the reflected light, which is difficult for wide field imaging. By applying time encoded spectroscopy technology, depth information can be obtained at high speed through time information of reflected light. As a result, we could obtain the 3D surface shape without scanning by measuring the reflected light through the CCD over time.
A multi-spectral laser speckle contrast imaging (MS-LSCI) system is proposed using only a single wavelength-swept laser, which provides both highly coherent and multi-spectral outputs to simultaneously generate laser speckle contrast images and multi-spectral images, respectively. Using a laser light swept from 770 to 821 nm at a repetition rate of 5 Hz and a CCD camera of 335 fps, 67 multi-spectral frame images are acquired in 0.76 nm wavebands over 51 nm spectral range. The spectral sub-windowing method of single wavelength-swept laser source is used to solve the lack of spectral information from a few individual light sources, which is a limitation of conventional MS-LSCI systems. In addition to the speckle flow index from the LSCI frames, the multi-spectrally encoded images can generate additional images of spectral absorbance. To further examine the performance of the MS-LSCI system, an in vivo cuff-induced ischemia experiment was conducted to show the real-time imaging of hemodynamic and blood oxygen saturation changes simultaneously over the entire 2.5 cm × 4.5 cm field of view.