Prof. Hedi Mattoussi Profile

Prof. Hedi Mattoussi

Research Physicist at Florida State Univ

SPIE Involvement:

Conference Program Committee | Author

Publications (43)

PROCEEDINGS ARTICLE | February 23, 2018

Surface characterization of hydrophobic core-shell QDs using NMR techniques

Proc. SPIE. 10507, Colloidal Nanoparticles for Biomedical Applications XIII

KEYWORDS: FT-IR spectroscopy, Spectroscopy, Molecules, Diffusion, Zinc, Quantum dots, Mass spectrometry, Molecular spectroscopy, Nanocrystals, Natural surfaces

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PROCEEDINGS ARTICLE | February 23, 2018

Anti-microbial peptide facilitated cytosolic delivery of metallic gold nanomaterials

Proc. SPIE. 10507, Colloidal Nanoparticles for Biomedical Applications XIII

KEYWORDS: Gold, Biomedical optics, Visualization, Nanoparticles, Polymers, Luminescence, Nanomaterials, Nanocrystals, Absorption, Nanorods

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PROCEEDINGS ARTICLE | April 24, 2017

Bio-orthogonal coupling on PEG-modified quantum dots (Conference Presentation)

Proc. SPIE. 10078, Colloidal Nanoparticles for Biomedical Applications XII

KEYWORDS: Semiconductors, Gold, Nanoparticles, Metals, Spectroscopy, Quantum dots, Chromophores, Nanocrystals, Mass attenuation coefficient, Absorption

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PROCEEDINGS ARTICLE | February 22, 2017

Peptide mediated intracellular delivery of semiconductor quantum dots

Proc. SPIE. 10078, Colloidal Nanoparticles for Biomedical Applications XII

KEYWORDS: Semiconductors, Confocal microscopy, Proteins, Cancer, Nanoparticles, Microscopy, Luminescence, Chemistry, Control systems, Quantum dots, Flow cytometry, Digital image correlation, Live cell imaging, Nanocrystals

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PROCEEDINGS ARTICLE | April 22, 2016

Design of a multi-coordinating polymer as a platform for functionalizing metal, metal oxide and semiconductor nanocrystals

Proc. SPIE. 9722, Colloidal Nanoparticles for Biomedical Applications XI

KEYWORDS: Gold, Oxides, Proteins, Iron, Nanoparticles, Polymers, Metals, Luminescence, Fluorescence resonance energy transfer, Nanocrystals

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PROCEEDINGS ARTICLE | April 22, 2016

Growth of fluorescence gold clusters using photo-chemically activated ligands

Proc. SPIE. 9722, Colloidal Nanoparticles for Biomedical Applications XI

KEYWORDS: Gold, Nanoparticles, Metals, Ultraviolet radiation, Luminescence, Molecules, Mass spectrometry, Sodium, Nanocrystals, Absorption

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PROCEEDINGS ARTICLE | March 12, 2015

Understanding the redox coupling between quantum dots and the neurotransmitter dopamine in hybrid self-assemblies

Proc. SPIE. 9338, Colloidal Nanoparticles for Biomedical Applications X

KEYWORDS: Signal attenuation, Dispersion, Luminescence, Quantum dots, Picosecond phenomena, Time resolved spectroscopy, Nanocrystals, Neurotransmitters, Absorption, Oxidation

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PROCEEDINGS ARTICLE | March 12, 2015

Quantifying the density of surface capping ligands on semiconductor quantum dots

Proc. SPIE. 9338, Colloidal Nanoparticles for Biomedical Applications X

KEYWORDS: Semiconductors, Nanoparticles, Metals, Chemistry, Quantum dots, Chromophores, Absorbance, In vivo imaging, Nanocrystals, Absorption

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PROCEEDINGS ARTICLE | March 24, 2014

Combining ligand design and photo-ligation to provide optimal quantum dot-bioconjugates for sensing and imaging

Proc. SPIE. 8955, Colloidal Nanoparticles for Biomedical Applications IX

KEYWORDS: Semiconductors, Proteins, Spectroscopy, Ultraviolet radiation, Nitrogen, Optical spectroscopy, Sodium, Nanocrystals, Chromatography, Absorption

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PROCEEDINGS ARTICLE | March 24, 2014

Shielding of quantum dots using diblock copolymers: implementing copper catalyzed click chemistry to fluorescent quantum dots

Proc. SPIE. 8955, Colloidal Nanoparticles for Biomedical Applications IX

KEYWORDS: Nanoparticles, Polymers, Luminescence, Copper, Ions, Coating, Chemistry, Quantum dots, Polymerization, Nanocrystals

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PROCEEDINGS ARTICLE | March 24, 2014

Multidentate oligomeric ligands to enhance the biocompatibility of iron oxide and other metal nanoparticles

Proc. SPIE. 8955, Colloidal Nanoparticles for Biomedical Applications IX

KEYWORDS: Oxides, Iron, Nanoparticles, Polymers, Metals, Molecules, Magnetism, Transmission electron microscopy, Nanocrystals, Absorption

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PROCEEDINGS ARTICLE | February 22, 2013

One phase growth of in-situ functionalized gold and silver nanoparticles and luminescent nanoclusters

Proc. SPIE. 8595, Colloidal Nanocrystals for Biomedical Applications VIII

KEYWORDS: Gold, Near infrared, Nanoparticles, Metals, Ultraviolet radiation, Luminescence, Silver, Transmission electron microscopy, Absorbance, Absorption

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PROCEEDINGS ARTICLE | May 5, 2010

Quantum dots in life sciences: applications, benefits, and safety issues

Proc. SPIE. 7666, Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX

KEYWORDS: Semiconductors, Safety, Polymers, Spectroscopy, Quantum efficiency, Quantum dots, Life sciences, Biology, Optical spectroscopy, Analytical research

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PROCEEDINGS ARTICLE | February 18, 2010

Optical size determination of quantum dots using FRET with terbium complexes as donors

Proc. SPIE. 7575, Colloidal Quantum Dots for Biomedical Applications V

KEYWORDS: Semiconductors, Spectroscopy, Luminescence, Multiplexing, Quantum dots, Terbium, Fluorescence resonance energy transfer, Signal detection, Single molecule spectroscopy, Absorption

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PROCEEDINGS ARTICLE | February 18, 2010

Delivery of quantum dot bioconjugates to the cellular cytosol: release from the endolysosomal system

Proc. SPIE. 7575, Colloidal Quantum Dots for Biomedical Applications V

KEYWORDS: Semiconductors, Proteins, Tissues, Polymers, Quantum dots, Toxicity, Cell biology, Analytical research, Sodium, Plasma

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To realize their full potential as intracellular imaging and sensing reagents, robust and efficient methods for the targeted cellular delivery of luminescent semiconductor quantum dots (QDs) must be developed. We have previously shown that QDs decorated with histidine-terminated polyarginine cell-penetrating peptides (CPP) are rapidly and specifically internalized via endocytosis by several mammalian cell lines with no cytotoxicity. Here we demonstrate the long-term intracellular stability and fate of these QD-peptide conjugates. We found that the QD-peptide conjugates remain sequestered within endolysosomal vesicles for up to three days after delivery. However, the CPP appeared to remain stably associated with the QD within these acidic vesicles over this time period. Hence, we explored a number of techniques to either actively deliver QDs directly to the cytosol or to facilitate the endosomal release of endocytosed QDs to the cytosol. Active methods (e.g., electroporation) delivered only modest amounts of QDs to the cytosol that appeared to form aggregates. Delivery of QDs using polymer-based transfection reagents resulted primarily in the endosomal sequestration of the QDs, although one commercial polymer tested delivered QDs to the cytosol but only after several days in culture and with a considerable degree of polymer-induced toxicity. Finally, a modular, amphiphilic peptide containing functionalities designed for cell penetration and vesicular membrane interaction demonstrated the ability to deliver QDs in a well-dispersed manner to the cytosol. This peptide mediated rapid QD uptake followed by a slower efficient endosomal release of the QDs to the cytosol that peaked at 48 hours post-delivery. Importantly, this QD-peptide conjugate elicited minimal cytotoxicity in two cell lines tested. A more detailed understanding of the mechanism of the peptide's uptake and endosomal escape attributes will lead to the design of further QD conjugates for targeted imaging and sensing applications.

PROCEEDINGS ARTICLE | March 3, 2009

Peptide linkers for the assembly of semiconductor quantum dot bioconjugates

Proc. SPIE. 7189, Colloidal Quantum Dots for Biomedical Applications IV

KEYWORDS: Semiconductors, Gold, Proteins, Quantum efficiency, Quantum dots, Distance measurement, Fluorescent proteins, Fluorescence resonance energy transfer, Nanolithography, In vitro testing

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PROCEEDINGS ARTICLE | March 3, 2009

Using metal complex-labeled peptides for charge transfer-based biosensing with semiconductor quantum dots

Proc. SPIE. 7189, Colloidal Quantum Dots for Biomedical Applications IV

KEYWORDS: Semiconductors, Biosensing, Metals, Luminescence, Quantum dots, Nanocrystals, Environmental sensing, Ruthenium, Absorption, Oxidation

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PROCEEDINGS ARTICLE | March 3, 2009

Intracellular delivery of and sensing with quantum dot bioconjugates

Proc. SPIE. 7189, Colloidal Quantum Dots for Biomedical Applications IV

KEYWORDS: Semiconductors, Polymers, Luminescence, Quantum efficiency, Multiplexing, Quantum dots, Toxicity, Receptors, Analytical research, Absorption

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PROCEEDINGS ARTICLE | March 3, 2009

Multivalent display of DNA conjugates on semiconductor quantum dots utilizing a novel conjugation method

Proc. SPIE. 7189, Colloidal Quantum Dots for Biomedical Applications IV

KEYWORDS: Semiconductors, Biomedical optics, Sensors, Nanoparticles, Particles, Chemistry, Quantum dots, In vivo imaging, Nanocrystals, Atmospheric particles

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PROCEEDINGS ARTICLE | February 19, 2009

Energy transfer-based biosensing of protease activity measured using an electroluminescent platform

Proc. SPIE. 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems

KEYWORDS: Semiconductors, Biosensing, Medicine, Sensors, Luminescence, Electroluminescence, Quantum dots, Charge-coupled devices, Fluorescence resonance energy transfer, Bandpass filters

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PROCEEDINGS ARTICLE | April 15, 2008

Self-assembled quantum dot-bioconjugates: characterization and use for sensing proteolytic activity

Proc. SPIE. 6945, Optics and Photonics in Global Homeland Security IV

KEYWORDS: Semiconductors, Proteins, Biosensing, Luminescence, Energy transfer, Chemistry, Quantum dots, Biology, Fluorescence resonance energy transfer, Nanocrystals

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PROCEEDINGS ARTICLE | March 13, 2008

Assembly and intracellular delivery of quantum dot-fluorescent protein bioconjugates

Proc. SPIE. 6866, Colloidal Quantum Dots for Biomedical Applications III

KEYWORDS: Proteins, Sensors, Nanoparticles, Luminescence, Quantum dots, Digital image correlation, Fluorescent proteins, Fluorescence resonance energy transfer, Nanocrystals, Nanolithography

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PROCEEDINGS ARTICLE | February 26, 2008

Specific cellular delivery and intracellular fate of quantum dot-peptide and quantum dot-polymer nanoassemblies

Proc. SPIE. 6866, Colloidal Quantum Dots for Biomedical Applications III

KEYWORDS: Semiconductors, Biomedical optics, Polymers, Metals, Spectroscopy, Luminescence, Ions, Quantum dots, Toxicity, Biology

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PROCEEDINGS ARTICLE | February 22, 2008

New ligand design to promote water compatibility of luminescent quantum dots and gold nanoparticles

Proc. SPIE. 6866, Colloidal Quantum Dots for Biomedical Applications III

KEYWORDS: Gold, Silica, Optical properties, Nanoparticles, Luminescence, Nitrogen, Quantum dots, Sodium, Nanocrystals, Absorption

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PROCEEDINGS ARTICLE | February 27, 2007

Selective cellular delivery of self-assembled quantum dot-peptide bioconjugates

Proc. SPIE. 6448, Colloidal Quantum Dots for Biomedical Applications II

KEYWORDS: Semiconductors, Luminescence, Quantum efficiency, Control systems, Quantum dots, Toxicity, Analytical research, Sodium, Signal detection, Absorption

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PROCEEDINGS ARTICLE | February 27, 2007

Single quantum dot fluorescence resonant energy transfer: probing the heterogeneity in macroscopic samples

Proc. SPIE. 6448, Colloidal Quantum Dots for Biomedical Applications II

KEYWORDS: Energy efficiency, Proteins, Particles, Luminescence, Molecules, Energy transfer, Quantum dots, Resonance energy transfer, Fluorescence resonance energy transfer, Absorption

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PROCEEDINGS ARTICLE | February 27, 2007

Quantum dot-DNA bioconjugates for fluorescence-resonance-energy-transfer-based biosensing

Proc. SPIE. 6448, Colloidal Quantum Dots for Biomedical Applications II

KEYWORDS: Semiconductors, Proteins, Biosensing, Luminescence, Quantum efficiency, Quantum dots, Resonance energy transfer, Analytical research, Fluorescence resonance energy transfer, Absorption

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PROCEEDINGS ARTICLE | March 27, 2006

Quantum-dot-based nanosensors designed for proteolytic monitoring

Proc. SPIE. 6096, Colloidal Quantum Dots for Biomedical Applications

KEYWORDS: Nanosensors, Proteins, Sensors, Luminescence, Quantum dots, Explosives, Biological research, Fluorescence resonance energy transfer, Nanocrystals, Absorption

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PROCEEDINGS ARTICLE | March 27, 2006

Hydrodynamic sizes of functional hydrophilic QDs

Proc. SPIE. 6096, Colloidal Quantum Dots for Biomedical Applications

KEYWORDS: Proteins, Nanoparticles, Polymers, Particles, Diffusion, Coating, Quantum dots, Dynamic light scattering, Nanocrystals, Signal detection

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PROCEEDINGS ARTICLE | March 27, 2006

Quantum dot-based fluorescence resonance energy transfer multiplexing: applications for biosensing

Proc. SPIE. 6096, Colloidal Quantum Dots for Biomedical Applications

KEYWORDS: Nanosensors, Proteins, Biosensing, Luminescence, Composites, Multiplexing, Quantum dots, Deconvolution, Fluorescence resonance energy transfer, Absorption

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PROCEEDINGS ARTICLE | March 27, 2006

Probing the effects of spectral overlap on quantum-dot-based FRET: Ensemble and single molecule studies

Proc. SPIE. 6096, Colloidal Quantum Dots for Biomedical Applications

KEYWORDS: Semiconductors, Proteins, Luminescence, Molecules, Energy transfer, Quantum dots, Resonance energy transfer, Fluorescence resonance energy transfer, Nanocrystals, Absorption

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PROCEEDINGS ARTICLE | April 28, 2005

Synthesis of surface ligands to prepare hydrophilic and biologically compatible quantum dots

Proc. SPIE. 5705, Nanobiophotonics and Biomedical Applications II

KEYWORDS: Oxides, Magnesium, Nanoparticles, Luminescence, Molecules, Resistance, Bioalcohols, Quantum dots, Nanocrystals, Natural surfaces

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PROCEEDINGS ARTICLE | April 28, 2005

A fluorescence resonance energy transfer quantum dot explosive nanosensor

Proc. SPIE. 5705, Nanobiophotonics and Biomedical Applications II

KEYWORDS: Nanosensors, Proteins, Sensors, Luminescence, Biosensors, Quantum dots, Explosives, Fluorescence resonance energy transfer, Chemical elements, Analog electronics

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PROCEEDINGS ARTICLE | April 28, 2005

Use of fluorescent quantum dots for studying live cells and organisms

Proc. SPIE. 5705, Nanobiophotonics and Biomedical Applications II

KEYWORDS: Proteins, Biomedical optics, Tumors, Luminescence, Molecules, Organisms, Quantum dots, Live cell imaging, In vivo imaging, In vitro testing

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PROCEEDINGS ARTICLE | April 28, 2005

Are luminescent quantum dots efficient energy acceptors?

Proc. SPIE. 5705, Nanobiophotonics and Biomedical Applications II

KEYWORDS: Proteins, Luminescence, Molecules, Energy transfer, Quantum efficiency, Quantum dots, Excitons, Time resolved spectroscopy, Fluorescence resonance energy transfer, Absorption

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PROCEEDINGS ARTICLE | April 4, 2005

Quantum-dot-based multiplexed fluorescence resonance energy transfer

Proc. SPIE. 5704, Genetically Engineered and Optical Probes for Biomedical Applications III

KEYWORDS: Proteins, Luminescence, Composites, Biosensors, Multiplexing, Quantum dots, Deconvolution, Fluorescence resonance energy transfer, Signal detection, Absorption

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PROCEEDINGS ARTICLE | December 29, 2004

Modulation of quantum dot photoemission based on fluorescence resonance energy transfer to a photochromic dye acceptor

Proc. SPIE. 5593, Nanosensing: Materials and Devices

KEYWORDS: Surface plasmons, Proteins, Switching, Modulation, Ultraviolet radiation, Luminescence, Molecules, Quantum dots, Fluorescence resonance energy transfer, Absorption

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PROCEEDINGS ARTICLE | November 5, 2002

Synthesis and characterization of colloidal mercury chacogenide quantum dots

Proc. SPIE. 4808, Optical Properties of Nanocrystals

KEYWORDS: Cadmium, Crystals, Mercury, Quantum efficiency, Chalcogenides, Quantum dots, Nanocrystals, Selenium, Edge emitting semiconductor lasers, Absorption

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PROCEEDINGS ARTICLE | March 18, 2002

Application of luminescent CdSe-ZnS quantum dot bioconjugates in immuno- and fluorescence-quenching assays

Proc. SPIE. 4636, Nanoscience Using Laser-Solid Interactions

KEYWORDS: Proteins, Luminescence, Molecules, Energy transfer, Resistance, Quantum dots, Receptors, Sodium, Nanocrystals, Absorption

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PROCEEDINGS ARTICLE | June 18, 2001

Bioconjugates of luminescent CdSe-ZnS quantum dots with an engineered two-domain protein G for use in fluoroimmunoassays

Proc. SPIE. 4258, Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications

KEYWORDS: Semiconductors, Visible radiation, Proteins, Nanoparticles, Luminescence, Resistance, Linear filtering, Quantum dots, Chlorine, Nanocrystals

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PROCEEDINGS ARTICLE | December 16, 1998

Electroluminescence and cathodoluminescence from inorganic CdSe nanocrystals embedded in thin films

Proc. SPIE. 3476, Organic Light-Emitting Materials and Devices II

KEYWORDS: Thin films, Luminescence, Composites, Zinc, Electroluminescence, Excitons, Nanocrystals, Heterojunctions, Thin film devices, Absorption

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PROCEEDINGS ARTICLE | December 16, 1998

Absolute photoluminescence quantum yield of molecular organic thin films: effects of doping with strongly fluorescent rubrene

Proc. SPIE. 3476, Organic Light-Emitting Materials and Devices II

KEYWORDS: Thin films, Luminescence, Molecules, Composites, Quantum efficiency, Doping, Solids, Aluminum, Telescopic pixel displays, Absorption

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PROCEEDINGS ARTICLE | December 16, 1998

Dye-doped molecular light-emitting diodes with enhanced performance

Proc. SPIE. 3476, Organic Light-Emitting Materials and Devices II

KEYWORDS: External quantum efficiency, Luminescence, Molecules, Composites, Quantum efficiency, Doping, Electroluminescence, Solids, Excitons, Telescopic pixel displays

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