Electrical Impedance Tomography (EIT) is a method used to record the impedance distribution within a target. The best-known application of EIT is lung diagnostics using imaging algorithms. However, apart from this, there are individual research projects dealing with imaging analysis at the cellular level. For example, cell analysis using EIT can help to distinguish diseased cells from healthy cells. To achieve this goal, an existing EIT system was combined with a new EIT chip in a first step. This chip allows analysis in very small dimensions. Various parameters such as the diameter of the measuring environment, the necessary conductive solutions or the measuring methods used were varied and evaluated. In a next step, various image reconstructions were carried out using data acquired with C. elegans.
During long-term imaging, cells move out of the field of view. We have generated functionalized substrates containing rectangular areas, which were capable in keeping cells over the whole observation period.
The major problem of Digital Holographic Microscopy (DHM) long term live cell imaging is that over time most of the tracked cells move out of the image area and other ones move in. Therefore, most of the cells are lost for the evaluation of individual cellular processes. Here, we present an effective solution for this crucial problem of long-term microscopic live cell analysis. We have generated functionalized slides containing areas of 250 μm per 200 μm. These micropatterned biointerfaces consist of passivating polyaclrylamide brushes (PAAm). Inner areas are backfilled with octadecanthiol (ODT), which allows cell attachment. The fouling properties of these surfaces are highly controllable and therefore the defined areas designed for the size our microscopic image areas were effective in keeping all cells inside the rectangles over the selected imaging period.