We present the in-vivo imaging of the global mouse brain ischemia using Bessel beam optical coherence microscopy. This method allows to monitor changes in brain structure with extra control of blood flow during the process of artery occlusion. The results show the capability and sensitivity of OCM system with Bessel beam to analyze brain plasticity after severe injury within a period of 8 days.
We used Optical Coherence Microscopy (OCM) to monitor structural and functional changes due to ischemic stroke in
small animals brains in vivo. To obtain lateral resolution of 2.2 μm over the range of 600 μm we used extended focus
configuration of OCM instrument involving Bessel beam. It provided access to detailed 3D information about the
changes in brain vascular system up to the level of capillaries across I and II/III layers of neocortex. We used
photothrombotic stroke model involving photoactive application of rose bengal to assure minimal invasiveness of the
procedure and precise localization of the clot distribution center. We present the comparative analysis involving
structural and angiographic maps of the stroke-affected brain enabling in-depth insight to the process of development of