Ms. Hui Chong Lau Profile

Hui Chong Lau

at Kyungpook National Univ

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Proceedings Article | 20 May 2013 Paper

LUSH-based SPR sensor for the detection of alcohols and pheromone

Hui-Chong Lau, Yeon-Kyung Lee, Jae-Young Kwon, Young-Soo Sohn, Jeong Ok Lim

Proceedings Volume 8879, 88790F (2013) https://doi.org/10.1117/12.2018709

KEYWORDS: Sensors, Proteins, Bioalcohols, Gold, Biosensing, Surface plasmons, Imaging spectroscopy, Biomedical optics, Molecules, Receptors

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Protein is a widely used sensing substrate in the biosensing technology. In the study conducted here, we used odorant binding protein, LUSH from Drosophila as a biosensing substrate in a miniaturized surface plasmon resonance (SPR) sensor. LUSH contains the specific alcohols binding sites, which mediates the detection of alcohols and pheromone. We first modified the surface of the gold sensor chip using the self assembled monolayer in the chloroform solution. The saturated concentration was determined prior to the detection of alcohols and pheromone at various concentrations. The results showed that the LUSH was saturated at 1000 μg/ml on the gold sensor chip. The detection response of LUSH was significant at higher concentration of alcohols. LUSH detected ethanol at concentration ≥50%; propanol was detected at ≥25% whereas pheromone was detected at ≥1.25 μg/μl. The results provide some fundamental information on the potential use of LUSH-based SPR as a simple and easy protein-based sensor in the near future.

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