We employed atomic force microscopy (AFM) with bias control to fabricate oxided nanopatterns on silicon surface with feature size down to 50nm. The relationship of silicon dioxide nanopatterns against humidity was studied and then the optimal parameter was used to make oxide nanoarry for interaction of biotin and streptavidin. The scanning function of AFM was utilized to verify the different height of biomolecules. According to our experimental results, using nano biochip of silicon dioxide can decrease the monitoring scale to nanometer and can be the nano-platform for monitoring the behavior of biomolecular interaction. We anticipate mimicking the correlation of single molecular behavior and an array of biomolecular behavior to understand the coincidence of them.
SERS method for biomolecular analysis has several potentials and advantages over traditional biochemical approaches, including less specimen contact, non-destructive to specimen, and multiple components analysis. Urine is an easily available body fluid for monitoring the metabolites and renal function of human body. We developed surface-enhanced Raman scattering (SERS) technique using 50nm size gold colloidal particles for quantitative human urine creatinine measurements. This paper shows that SERS shifts of creatinine (104mg/dl) in artificial urine is from 1400cm<sup>-1</sup> to 1500cm<sup>-1</sup> which was analyzed for quantitative creatinine measurement. Ten human urine samples were obtained from ten healthy persons and analyzed by the SERS technique. Partial least square cross-validation (PLSCV) method was utilized to obtain the estimated creatinine concentration in clinically relevant (55.9mg/dl to 208mg/dl) concentration range. The root-mean square error of cross validation (RMSECV) is 26.1mg/dl. This research demonstrates the feasibility of using SERS for human subject urine creatinine detection, and establishes the SERS platform technique for bodily fluids measurement.