Proceedings Article | 7 March 2005
Proc. SPIE. 5702, Optical Diagnostics and Sensing V
KEYWORDS: Refractive index, Light sources, Scattering, Luminescence, Molecules, Light scattering, Collimation, Micro optics, Mie scattering, Atmospheric particles
In the work, the light source model for the fluorescence emission
in the biochips has been extensively studied such as to
effectively design the necessary micro-optic elements for the
fluorescence signal detection in biochips. With most advantaging
properties, the fluorescence technology does provide the high
sensitivity, response in real time, and multiple target labeling
for the applications in biochips. To practical applications, the
final signal detection is to measure the fluorescence emission. In
fact, the fluorescence emission process can be determined through
four stages of transformation; that is the excitation, the
absorption, the fluorescence conversion, and the fluorescence
scattering. As the total internal reflection configuration for the
fluorescence excitation is utilized, the evanescent waves are
introduced from different excitation sources in the viewpoints of
the principle analysis and the practical applications,
respectively. In such a way, the spatial intensity of the fluorescence emission is found not to be uniformly distributed,
and the performance of the micro-optic detection system thus
diversed deviated. Except that, the fluorescence emission is
further considered to include the extinction ratio and the quantum
yield of the fluorescent dyes and the scattering effect from the
molecules in the reaction solution. To the end, the precise
fluorescence emission model in the microstructure has been
obtained through the above 4 stages by the optic ray-tracing
simulation. Accordingly, one corresponding collimating lens has
been designed based on the new light source model.
