We propose a simple fluorescent bio-chip based on two types of alternative current-dielectrophoretic (AC-DEP) force, attractive (positive DEP) and repulsive (negative DEP) force, for simultaneous nano-molecules analysis. Various radius of micro-holes on the bio-chip are designed to apply the different AC-DEP forces, and the nano-molecules are concentrated inside the micro-hole arrays according to the intensity of the DEP force. The bio-chip was fabricated by Micro Electro Mechanical system (MEMS) technique, and was composed of two layers; a SiO<sub>2</sub> layer and Ta/Pt layer were accomplished for an insulation layer and a top electrode with micro-hole arrays to apply electric fields for DEP force, respectively. Each SiO<sub>2</sub> and Ta/Pt layers were deposited by thermal oxidation and sputtering, and micro-hole arrays were fabricated with Inductively Coupled Plasma (ICP) etching process. For generation of each positive and negative DEP at micro-holes, we applied two types of sine-wave AC voltage with different frequency range alternately. The intensity of the DEP force was controlled by the radius of the micro-hole and size of nano-molecule, and calculated with COMSOL multi-physics. Three types of nano-molecules labelled with different fluorescent dye were used and the intensity of nano-molecules was examined by the fluorescent optical analysis after applying the DEP force. By analyzing the fluorescent intensities of the nano-molecules, we verify the various nano-molecules in analyte are located successfully inside corresponding micro-holes with different radius according to their size.
Stereo video becomes an important issue with the developments of 3D display technologies. While a stereo system provides the perception of 3D depth, the amount of data for stereo video may be doubled compared to mono video. So an efficient stereo video coding technique is essential. Since the stereo video is taken from the same object in the two different views, most of objects in a stereo pair are translated only to the horizontal direction, while objects in
subsequent frames of a mono video can be translated to an arbitrary direction, rotated, and zoomed. Hence, unlike in the mono video, the disparity in a stereo pair can be well estimated by a translational motion or disparity if the object boundary is exactly described. In this paper, we propose an efficient disparity estimation scheme based on a edge model describing object boundary in a block, and apply the estimated disparity to stereo video compression. In addition, a
disparity regularization scheme, which is proper for the edge model, is proposed to reduce the bits required for coding the block motion vectors and disparity values. It has been found that the proposed algorithms significantly improve the coding efficiency of stereo video sequences.