Prof. Ignacy Gryczynski Profile

Prof. Ignacy Gryczynski

Professor and Director of Microscopy Core Facility at Univ of North Texas Health Science Ctr

SPIE Involvement:

Author

Publications (59)

Proceedings Article | 9 March 2020 Presentation

From multi-photon absorption and stimulated emission to forbidden S0→T1 transition (Conference Presentation)

Zygmunt Gryczynski, Ignacy Gryczynski

Proceedings Volume 11244, 112441I (2020) https://doi.org/10.1117/12.2551828

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Proceedings Article | 4 March 2019 Presentation

Increasing the sensitivity and resolution of single plane light sheet microscopy with multi-pulse pumping and time-gated detection (Conference Presentation)

Joseph Kimball, Zhangatay Nurekeyev, Jose Chavez, Luca Ceresa, Sangram Raut, Ignacy Gryczynski, Zygmunt Gryczynski

Proceedings Volume 10884, 1088405 (2019) https://doi.org/10.1117/12.2514298

KEYWORDS: Microscopy, Signal processing, Optical microscopes, Signal detection, Image quality, Fluorescent markers

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SPIE Journal Paper | 22 June 2018 Open Access

Surface plasmon-assisted microscope

Julian Borejdo, Zygmunt Gryczynski, Rafal Fudala, Chaitanya Joshi, Kathleen Borgmann, Anuja Ghorpade, Ignacy Gryczynski

JBO, Vol. 23, Issue 06, 060502, (June 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.6.060502

KEYWORDS: Luminescence, Metals, Gold, Microscopes, Surface plasmons, Glasses, Objectives, Molecules, Refractive index, Optical spheres

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Proceedings Article | 14 March 2018 Presentation

Multi-pulse based approach to study dynamics of molecular assemblies with subwavelength resolution (Conference Presentation)

Zhangatay Nurekeyev, Hung Doan, Zygmunt Gryczynski, Rafal Fudala, Ignacy Gryczynski, Julian Borejdo, Dorota Stankowska, Sergei Dzyuba

Proceedings Volume 10500, 105000H (2018) https://doi.org/10.1117/12.2299436

KEYWORDS: Molecular assembly, Real time imaging, Molecules, Optical microscopy, Image resolution, Ultrafast imaging, Super resolution, In vitro testing, In vivo imaging, Biomedical optics

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Proceedings Article | 1 March 2016 Paper

Novel plasmonic platform for ultra-sensitive detection and diagnostics

Sangram Raut, Ryan Rich, Tanya Shtoyko, Sebastian Requena, Ilkay Bora, Bo Laursen, Thomas Just Sørensen, Julian Borejdo, Zygmunt Gryczynski, Ignacy Gryczynski

Proceedings Volume 9714, 971419 (2016) https://doi.org/10.1117/12.2221923

KEYWORDS: Luminescence, Plasmonics, Fractal analysis, Glasses, Silver, Metals, Diagnostics, Quantum efficiency, Nanostructures, Absorption, Proteins

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Proceedings Article | 1 March 2016 Paper

Multi-pulse pumping for far-field super-resolution imaging

Sebastian Requena, Sangram Raut, Hung Doan, Joe Kimball, Rafal Fudala, Julian Borejdo, Ignacy Gryczynski, Yuri Strzhemechny, Zygmunt Gryczynski

Proceedings Volume 9714, 97140F (2016) https://doi.org/10.1117/12.2221921

KEYWORDS: Nanoparticles, Super resolution, Luminescence, Microscopy, Image resolution, Microscopes, Diffraction, Stimulated emission depletion microscopy, Stochastic processes, Confocal microscopy

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Proceedings Article | 4 March 2014 Paper

Fluorescent nanodiamonds for ultrasensitive detection

Joseph Kimball, Dmytro Shumilov, Badri Maliwa, T. Zerda, Bibhu Rout, Rafal Fudala, Sangram Raut, Ignacy Gryczynski, Eric Simanek, Julian Borejdo, Ryan Rich, Irina Akopova, Zygmunt Gryczynski

Proceedings Volume 8950, 89501H (2014) https://doi.org/10.1117/12.2045376

KEYWORDS: Luminescence, Diamond, Ions, Nitrogen, Proteins, Sensors, Chemical species, Resonance enhancement, Carbon, Annealing

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Proceedings Article | 4 March 2014 Paper

Simple multipulse excitation for enhanced detection of long-lived fluorophores

Joseph Kimball, Dmytro Shumilov, Rafal Fudala, Sangram Raut, Ignacy Gryczynski, Julian Borejdo, Irina Akopova, Ryszard Grygorczyk, Zygmunt Gryczynski

Proceedings Volume 8950, 895005 (2014) https://doi.org/10.1117/12.2045432

KEYWORDS: Molecules, Signal detection, Glasses, Neon, Scattering, Laser sources, Quantum efficiency, Picosecond phenomena, Signal attenuation, Semiconductor lasers

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Proceedings Article | 22 February 2013 Paper

Detection of hyaluronidase activity using fluorescence lifetime correlation spectroscopy to separate diffusing species and eliminate autofluorescence

Ryan Rich, Mark Mummert, Zygmunt Gryczynski, Julian Borejdo, Ignacy Gryczynski, Thomas Sørensen, Bo Laursen, Rafal Fudala

Proceedings Volume 8590, 859003 (2013) https://doi.org/10.1117/12.2007261

KEYWORDS: Fluorescence correlation spectroscopy, Diffusion, Luminescence, Rhodamine, Photons, Molecules, Data modeling, Fluorescence spectroscopy, Spectroscopy, Calibration

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SPIE Journal Paper | 19 January 2012 Open Access

Extending Förster resonance energy transfer measurements beyond 100 Å using common organic fluorophores: enhanced transfer in the presence of multiple acceptors

Badri Maliwal, Sangram Raut, Rafal Fudala, Ignacy Gryczynski, Sabato D'Auria, Vincenzo Marzullo, Alberto Luini, Zygmunt Gryczynski

JBO, Vol. 17, Issue 1, 011006, (January 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.1.011006

KEYWORDS: Proteins, Fluorescence resonance energy transfer, Luminescence, Distance measurement, Molecules, Resonance energy transfer, Optical spheres, Lanthanides, Energy transfer, Absorption

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SPIE Journal Paper | 1 September 2010

Plasmonic platforms of self-assembled silver nanostructures in application to fluorescence

Rafal Luchowski, Nils Calander, Tanya Shtoyko, Elisa Apicella, Julian Borejdo, Zygmunt Gryczynski, Ignacy Gryczynski

JNP, Vol. 4, Issue 01, 043516, (September 2010) https://doi.org/10.1117/12.10.1117/1.3500463

KEYWORDS: Luminescence, Silver, Plasmonics, Glasses, Particles, Mirrors, Nanostructures, Rhodamine B, Metals, Microscopes

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Proceedings Article | 13 February 2010 Paper

Depolarized scattering of silver nanostructures in dye-less sensing

Pabak Sarkar, Tanya Shtoyko, Rafal Luchowski, Nils Calander, Aby Roth, Anne-Marie Brun-Zinkernagel, Zygmunt Gryczynski, Ignacy Gryczynski

Proceedings Volume 7574, 75740V (2010) https://doi.org/10.1117/12.841392

KEYWORDS: Scattering, Particles, Light scattering, Silver, Dielectrics, Nanoparticles, Spherical lenses, Nanostructures, Anisotropy, Nanorods

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SPIE Journal Paper | 1 January 2010 Open Access

Kinetics of a single cross-bridge in familial hypertrophic cardiomyopathy heart muscle measured by reverse Kretschmann fluorescence

Prasad Mettikolla, Nils Calander, Rafal Luchowski, Ignacy Gryczynski, Zygmunt Gryczynski, Julian Borejdo

JBO, Vol. 15, Issue 01, 017011, (January 2010) https://doi.org/10.1117/12.10.1117/1.3324871

KEYWORDS: Heart, Luminescence, Metals, Surface plasmons, Optical spheres, Glasses, Fluorescence correlation spectroscopy, Correlation function, Molecules, Light scattering

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SPIE Journal Paper | 1 May 2009 Open Access

Photophysical properties of Cerulean and Venus fluorescent proteins

Pabak Sarkar, Srinagesh Koushik, Steven Vogel, Ignacy Gryczynski, Zygmunt Gryczynski

JBO, Vol. 14, Issue 03, 034047, (May 2009) https://doi.org/10.1117/12.10.1117/1.3156842

KEYWORDS: Venus, Proteins, Fluorescent proteins, Luminescence, Anisotropy, Chromophores, Ultraviolet radiation, Fluorescence resonance energy transfer, Fluorescence anisotropy, Green fluorescent protein

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SPIE Journal Paper | 1 May 2008 Open Access

Reduction of photobleaching and photodamage in single molecule detection: observing single actin monomer in skeletal myofibrils

Julian Borejdo, Priya Muthu, John Talent, Zygmunt Gryczynski, Nils Calander, Irina Akopova, Tanya Shtoyko, Ignacy Gryczynski

JBO, Vol. 13, Issue 03, 034021, (May 2008) https://doi.org/10.1117/12.10.1117/1.2938689

KEYWORDS: Molecules, Glasses, Luminescence, Nanoparticles, Cameras, Silver, Objectives, Photons, Proteins, Signal to noise ratio

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Proceedings Article | 21 February 2008 Paper

Fluorescence enhancement on silver nanostructures: studies of components of ribosomal translation in vitro

Wlodek Mandecki, Shashank Bharill, Julian Borejdo, Diana Cabral, Barry Cooperman, Ian Farrell, Linus Fetter, Emanuel Goldman, Zygmunt Gryczynski, Hieronim Jakubowski, Hanqing Liu, Rafal Luchowski, Evgenia Matveeva, Dongli Pan, Haiou Qin, Donald Tennant, Ignacy Gryczynski

Proceedings Volume 6862, 68620T (2008) https://doi.org/10.1117/12.768612

KEYWORDS: Luminescence, Silver, Molecules, Nanostructures, Quartz, Glasses, Particles, Metals, Gold, In vitro testing

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SPIE Journal Paper | 1 January 2008 Open Access

Decreasing photobleaching by silver nanoparticles on metal surfaces: application to muscle myofibrils

Priya Muthu, Ignacy Gryczynski, Zygmunt Gryczynski, John Talent, Irina Akopova, Julian Borejdo

JBO, Vol. 13, Issue 01, 014023, (January 2008) https://doi.org/10.1117/12.10.1117/1.2854120

KEYWORDS: Silver, Mirrors, Luminescence, Glasses, Nanoparticles, Molecules, Gold, Metals, Confocal microscopy, Aluminum

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Proceedings Article | 10 February 2007 Paper

New surface plasmons approach to single molecule detection (SMD) and fluorescence correlation spectroscopy (FCS)

Zygmunt Gryczynski, I. Gryczynski, E. Matveeva, N. Calander, R. Grygorczyk, I. Akopova, S. Bharill, P. Muthu, S. Klidgar, J. Borejdo

Proceedings Volume 6444, 64440G (2007) https://doi.org/10.1117/12.715268

KEYWORDS: Luminescence, Metals, Surface plasmons, Fluorescence correlation spectroscopy, Molecules, Prisms, Silver, Fluorescence spectroscopy, Objectives, Interfaces

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Proceedings Article | 27 February 2006 Paper

Minimization of detection volume by surface plasmon-coupled emission

Z. Gryczynski, J. Borejdo, E. Matveeva, N. Calander, R. Grygorczyk, J. Harper, I. Gryczynski

Proceedings Volume 6092, 60920S (2006) https://doi.org/10.1117/12.673898

KEYWORDS: Luminescence, Prisms, Rhodamine B, Surface plasmons, Reflectivity, Quartz, Glasses, Metals, Microscopes, Bioalcohols

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Proceedings Article | 27 February 2006 Paper

Ultrasensitive detection in optically dense physiological media: applications to fast reliable biological assays

Evgenia Matveeva, Ignacy Gryczynski, Klaus Berndt, Joseph Lakowicz, Ewa Goldys, Zygmunt Gryczynski

Proceedings Volume 6092, 60920K (2006) https://doi.org/10.1117/12.648112

KEYWORDS: Blood, Signal detection, Luminescence, Glasses, Silver, Surface plasmons, Metals, Statistical analysis, Biomedical optics, Fluorescence spectroscopy

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Proceedings Article | 1 April 2005 Paper

Multicolor fluoroimmunoassays based on surface plasmon-coupled emission

Evgenia Matveeva, Joanna Malicka, Ignacy Gryczynski, Joseph Lakowicz, Zygmunt Gryczynski

Proceedings Volume 5692, (2005) https://doi.org/10.1117/12.593430

KEYWORDS: Metals, Glasses, Silver, Prisms, Surface plasmons, Reflectivity, Thin films, Luminescence, Refractive index, Plasmons

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Proceedings Article | 31 March 2005 Paper

Enhanced fluorescence of labeled proteins coated on silver nanoparticles

Joanna Lukomska, Evgenia Matveeva, Ignacy Gryczynski, Robert Flower, Joanna Malicka, Joseph Lakowicz, Slawomir Makowiec, Zygmunt Gryczynski

Proceedings Volume 5703, (2005) https://doi.org/10.1117/12.593426

KEYWORDS: Luminescence, Silver, Nanoparticles, Proteins, Glasses, Magnesium, Particles, Atmospheric particles, Cameras, Optical imaging

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Proceedings Article | 1 July 2004 Paper

Metal-enhanced fluorescence: a novel approach to ultra-sensitive fluorescence sensing assay platforms

Zygmunt Gryczynski, Joanna Malicka, Ignacy Gryczynski, Evgenia Matveeva, Chris Geddes, Kadir Aslan, Joseph Lakowicz

Proceedings Volume 5321, (2004) https://doi.org/10.1117/12.530072

KEYWORDS: Luminescence, Metals, Particles, Silver, Quantum efficiency, Absorption, Sensors, Refractive index, Fluorescence spectroscopy, Atmospheric particles

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Proceedings Article | 1 July 2004 Paper

DNA hybridization using surface plasmon-coupled emission

Joanna Malicka, Ignacy Gryczynski, Zygmunt Gryczynski, Joseph Lakowicz

Proceedings Volume 5321, (2004) https://doi.org/10.1117/12.530001

KEYWORDS: Metals, Surface plasmons, Silver, Glasses, Prisms, Proteins, Luminescence, Reflectivity, Quantum efficiency, Quartz

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Proceedings Article | 21 June 2004 Paper

Long-wavelength long-lifetime luminophores for cellular and tissue imaging

Zygmunt Gryczynski, Badri Maliwal, Ignacy Gryczynski, Joseph Lakowicz

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.533913

KEYWORDS: Quantum efficiency, Luminescence, Energy transfer, Energy efficiency, Resolution enhancement technologies, Absorption, Tissues, Fluorescence spectroscopy, Microscopy, Resonance energy transfer

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Proceedings Article | 16 June 2004 Paper

Metal-enhanced fluorescent biomarkers for cardiac risk assessment

Evgenia Matveeva, Zygmunt Gryczynski, Joanna Malicka, Ignacy Gryczynski, Joseph Lakowicz

Proceedings Volume 5327, (2004) https://doi.org/10.1117/12.530091

KEYWORDS: Glasses, Luminescence, Silver, Signal detection, Absorbance, Particles, Coating, Fluorescent markers, Heart, Medicine

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Proceedings Article | 16 June 2004 Paper

Surface plasmon-coupled directional fluorescence emission

Ignacy Gryczynski, Joanna Malicka, Zygmunt Gryczynski, Kazimierz Nowaczyk, Joseph Lakowicz

Proceedings Volume 5327, (2004) https://doi.org/10.1117/12.530048

KEYWORDS: Prisms, Surface plasmons, Metals, Silver, Luminescence, Refractive index, Glasses, Reflectivity, Absorption, Absorbance

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Proceedings Article | 16 June 2004 Paper

Advances in surface-enhanced fluorescence

Joseph Lakowicz, Chris Geddes, Ignacy Gryczynski, Joanna Malicka, Zygmunt Gryczynski, Kadir Aslan, Joanna Lukomska, Jun Huang

Proceedings Volume 5327, (2004) https://doi.org/10.1117/12.542458

KEYWORDS: Silver, Luminescence, Quartz, Particles, Absorption, Metals, Atmospheric particles, Glasses, Quantum efficiency, Nanorods

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Proceedings Article | 14 June 2004 Paper

Luminescent blinking from silver, gold, and copper nanostructures: a new class of probes for imaging and localization

Chris Geddes, Ignacy Gryczynski, Alex Parfenov, Kadir Aslan, Joseph Lakowicz

Proceedings Volume 5329, (2004) https://doi.org/10.1117/12.530063

KEYWORDS: Silver, Glasses, Gold, Luminescence, Copper, Nanostructures, Absorption, Fractal analysis, Microscopes, Electrodes

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SPIE Journal Paper | 1 July 2003 Open Access

Metal-enhanced emission from indocyanine green: a new approach to in vivo imaging

Joanna Malicka, Ignacy Gryczynski, Chris Geddes, Joseph Lakowicz

JBO, Vol. 8, Issue 03, (July 2003) https://doi.org/10.1117/12.10.1117/1.1578643

KEYWORDS: Silver, Quartz, Metals, Luminescence, Particles, Indocyanine green, Quantum efficiency, Molecules, Atmospheric particles, Absorption

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Proceedings Article | 21 June 2002 Paper

Biomedical applications of radiative decay engineering

Joseph Lakowicz, Ignacy Gryczynski, Joanna Malicka, Yibing Shen, Zygmunt Gryczynski

Proceedings Volume 4626, (2002) https://doi.org/10.1117/12.472114

KEYWORDS: Silver, Particles, Metals, Quantum efficiency, Rhodamine B, Luminescence, Absorption, Resonance energy transfer, Molecules, Biomedical optics

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SPIE Journal Paper | 1 September 2000

Simple apparatus for polarization sensing of analytes

Zygmunt Gryczynski, Ignacy Gryczynski, Joseph Lakowicz

OE, Vol. 39, Issue 09, (September 2000) https://doi.org/10.1117/12.10.1117/1.1286521

KEYWORDS: Polarizers, Polarization, Glucose, Sensors, Luminescence, Statistical analysis, Proteins, Optical engineering, Ultraviolet radiation

Proceedings Article | 18 November 1999 Paper

Fluorescence polarization sensing

Zygmunt Gryczynski, Ignacy Gryczynski, Joseph Lakowicz

Proceedings Volume 3857, (1999) https://doi.org/10.1117/12.370274

KEYWORDS: Polarization, Sensors, Luminescence, Polarizers, Oxygen, Calcium, Visualization, Statistical analysis, Measurement devices, Lamps

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SPIE Journal Paper | 1 October 1999 Open Access

Polarization-based sensing of glucose using an oriented reference film

Joseph Lakowicz, Ignacy Gryczynski, Zygmunt Gryczynski, Leah Tolosa, Lisa Randers-Eichhorn, Govind Rao

JBO, Vol. 4, Issue 04, (October 1999) https://doi.org/10.1117/12.10.1117/1.429955

KEYWORDS: Polarization, Glucose, Proteins, Sensors, Anisotropy, Luminescence, Polarizers, Holographic optical elements, Light sources, Biological and chemical sensing

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Proceedings Article | 3 May 1999 Paper

Novel methods for fluorescence sensing

Joseph Lakowicz, Ignacy Gryczynski, Zygmunt Gryczynski, Jonathan Dattelbaum, Leah Tolosa, Govind Rao

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347525

KEYWORDS: Polarization, Sensors, Oxygen, Anisotropy, Polarizers, Glucose, Luminescence, Silicon, Chemical analysis, Biological and chemical sensing

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Proceedings Article | 3 May 1999 Paper

Fluorescence sensing of glucose

Leah Tolosa, Ignacy Gryczynski, Lisa Randers-Eichhorn, Jonathan Dattelbaum, Govind Rao, Joseph Lakowicz

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347544

KEYWORDS: Glucose, Modulation, Proteins, Sensors, Luminescence, Ruthenium, Phase shift keying, Blood, Lithium, Phase measurement

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Proceedings Article | 1 May 1998 Paper

Multiphoton excitation of fluorescence

Joseph Lakowicz, Ignacy Gryczynski

Proceedings Volume 3256, (1998) https://doi.org/10.1117/12.307075

KEYWORDS: Anisotropy, Luminescence, Proteins, Absorption, Spectroscopy, Fluorescence spectroscopy, Fluorescence anisotropy, Photons, Sapphire lasers, Microscopy

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Proceedings Article | 1 May 1998 Paper

Fluorescence anisotropy controlled by light quenching

Ignacy Gryczynski, Zygmunt Gryczynski, Joseph Lakowicz

Proceedings Volume 3256, (1998) https://doi.org/10.1117/12.307076

KEYWORDS: Quenching (fluorescence), Anisotropy, Fluorescence anisotropy, Polarization, Luminescence, Molecules, Dye lasers, Absorption, Polarizers, Proteins

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Proceedings Article | 10 April 1998 Paper

Fluorescence detection of camptothecin anticancer drugs by two-photon excitation

Thomas Burke, Magda Malak, David Bom, Dennis Curran, Henryk Malak, Ignacy Gryczynski, Joseph Lakowicz

Proceedings Volume 3259, (1998) https://doi.org/10.1117/12.307334

KEYWORDS: Blood, Fluorescence spectroscopy, Plasma, Luminescence, Cancer, Absorption, Tissues, Polarization, Chemistry, Oncology

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Proceedings Article | 7 May 1997 Paper

Fluorescence detection of the anticancer drug topotecan and related camptothecins in plasma and whole blood by two-photon excitation

Thomas Burke, Henryk Malak, Ignacy Gryczynski, Joseph Lakowicz

Proceedings Volume 2980, (1997) https://doi.org/10.1117/12.273532

KEYWORDS: Plasma, Blood, Luminescence, Fluorescence spectroscopy, Cancer, Oncology, Tissues, Skin, Translucency, Near infrared

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Proceedings Article | 7 May 1997 Paper

Two-color two-photon excitation of fluorescence

Joseph Lakowicz, Ignacy Gryczynski, Henryk Malak, Zygmunt Gryczynski

Proceedings Volume 2980, (1997) https://doi.org/10.1117/12.273539

KEYWORDS: Luminescence, Anisotropy, Absorption, Picosecond phenomena, Signal processing, Polarization, Fluorescence anisotropy, Microscopy, Proteins, Dye lasers

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SPIE Journal Paper | 2 January 1997 Open Access

Wavelength-selective light quenching of biochemical fluorophores

Ignacy Gryczynski, Jozef Kusba, Joseph Lakowicz

JBO, Vol. 2, Issue 01, (January 1997) https://doi.org/10.1117/12.10.1117/12.259609

KEYWORDS: Quenching (fluorescence), Proteins, Macromolecules, Luminescence, Phase shift keying, Dye lasers, Solids, Modulation, Diffusion, Picosecond phenomena

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SPIE Journal Paper | 1 October 1996 Open Access

Three-photon excitation of 2,5-bis(4-biphenyl)oxazole: steady-state and time-resolved intensities and anisotropies

Ignacy Gryczynski, Henryk Malak, Stefan Hell, Joseph Lakowicz

JBO, Vol. 1, Issue 04, (October 1996) https://doi.org/10.1117/12.10.1117/12.250670

KEYWORDS: Anisotropy, Sapphire lasers, Fluorescence anisotropy, Photons, Modulation, Femtosecond phenomena, Luminescence, Absorption, Signal attenuation, Fluorescence spectroscopy

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SPIE Journal Paper | 2 January 1996 Open Access

Three-photon excitation in fluorescence microscopy

Stefan Hell, Karsten Bahlmann, Martin Schrader, Aleksi Soini, Henryk Malak, Ignacy Gryczynski, Joseph Lakowicz

JBO, Vol. 1, Issue 01, (January 1996) https://doi.org/10.1117/12.10.1117/12.229062

KEYWORDS: Luminescence, Microscopes, Microscopy, Absorption, Rhodamine, Photons, Sapphire lasers, Mode locking, Absorbance, Objectives

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