Dr. Igor L. Medintz Profile

Dr. Igor L. Medintz

Research Biologist at U S Naval Reserach Lab

SPIE Involvement:

Conference Program Committee | Conference Co-Chair | Author

Publications (49)

PROCEEDINGS ARTICLE | March 15, 2018

Probing kinetic enhancement of β-galactosidase-nanoparticle complexes (Conference Presentation)

Proc. SPIE. 10507, Colloidal Nanoparticles for Biomedical Applications XIII

KEYWORDS: Nanotechnology, Nanoparticles, Diffusion, Quantum dots, Biology, Cell biology, Systems modeling, Catalysis

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PROCEEDINGS ARTICLE | February 23, 2018

Nanoparticle bioconjugate for controlled cellular delivery of doxorubicin

Proc. SPIE. 10507, Colloidal Nanoparticles for Biomedical Applications XIII

KEYWORDS: Modulation, Nanoparticles, Chemistry, Control systems, Quantum dots, Toxicity

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PROCEEDINGS ARTICLE | August 29, 2017

DNA scaffold nanostructures for efficient and directional propagation of light harvesting cascades

Proc. SPIE. 10355, Nanobiosystems: Processing, Characterization, and Applications X

KEYWORDS: Nanostructures, Biophotonic applications, Fluorescence resonance energy transfer, Light harvesting, DNA photonics, DNA nanotechnology, Bionanoengineering

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PROCEEDINGS ARTICLE | February 22, 2017

Semiconductor quantum dots as Förster resonance energy transfer donors for intracellularly-based biosensors

Proc. SPIE. 10078, Colloidal Nanoparticles for Biomedical Applications XII

KEYWORDS: Semiconductors, Nanostructures, Proteins, Optical properties, Sensors, Molecules, Biosensors, Quantum dots, Resonance energy transfer, Live cell imaging, Fluorescent proteins, Fluorescence resonance energy transfer

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PROCEEDINGS ARTICLE | December 9, 2016

Using DNA nanostructures to harvest light and create energy transfer and harvesting systems

Proc. SPIE. 10013, SPIE BioPhotonics Australasia

KEYWORDS: Nanostructures, Luminescence, Molecules, Energy transfer, Energy harvesting, Resonance energy transfer, Fluorescence resonance energy transfer, Dendrimers, Light harvesting, Molecular photonics

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PROCEEDINGS ARTICLE | April 28, 2016

Energy transfer sensitization of luminescent gold nanoclusters: more than just the classical Forster mechanism (Conference Presentation)

Proc. SPIE. 9722, Colloidal Nanoparticles for Biomedical Applications XI

KEYWORDS: Semiconductors, Gold, Energy efficiency, Data modeling, Metals, Energy transfer, Quantum dots, Fluorescence resonance energy transfer, Nanocrystals, Nanolithography

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PROCEEDINGS ARTICLE | April 22, 2016

Quantum dot based enzyme activity sensors present deviations from Michaelis-Menten kinetic model

Proc. SPIE. 9722, Colloidal Nanoparticles for Biomedical Applications XI

KEYWORDS: Nanosensors, Sensors, Calibration, Nanoparticles, Luminescence, Quantum dots, Resonance energy transfer, Fluorescence resonance energy transfer, Signal detection, Systems modeling

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PROCEEDINGS ARTICLE | April 22, 2016

The influence of cell penetrating peptide branching on cellular uptake of QDs

Proc. SPIE. 9722, Colloidal Nanoparticles for Biomedical Applications XI

KEYWORDS: Semiconductors, Biosensing, Carbon dioxide, Tissues, Sensors, Nanoparticles, Luminescence, Quantum dots, Toxicity, Analytical research, Dendrimers, Neptunium

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PROCEEDINGS ARTICLE | October 12, 2015

Utilizing homogenous FRET to extend molecular photonic wires beyond 30 nm (Presentation Recording)

Proc. SPIE. 9545, Nanophotonic Materials XII

KEYWORDS: Nanostructures, Energy efficiency, Luminescence, Energy transfer, Energy harvesting, Resonance energy transfer, Fluorescence resonance energy transfer, Photosynthesis, Molecular photonics, Anisotropy

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PROCEEDINGS ARTICLE | August 21, 2015

Modified kinetics of enzymes interacting with nanoparticles

Proc. SPIE. 9550, Biosensing and Nanomedicine VIII

KEYWORDS: Sensors, Calibration, Nanoparticles, Luminescence, Molecules, Absorbance, Fluorescence resonance energy transfer, Neptunium, Molecular energy transfer, Catalysis

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PROCEEDINGS ARTICLE | August 21, 2015

Semiconductor quantum dots as delivery and imaging platforms for intracellular assembly

Proc. SPIE. 9550, Biosensing and Nanomedicine VIII

KEYWORDS: Semiconductors, Proteins, Optical properties, Visualization, Molecules, Ions, Quantum dots, Fluorescence resonance energy transfer, Neodymium, Nanolithography

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PROCEEDINGS ARTICLE | May 13, 2015

Enhancing enzymatic efficiency by attachment to semiconductor nanoparticles for biosensor applications

Proc. SPIE. 9487, Smart Biomedical and Physiological Sensor Technology XII

KEYWORDS: Semiconductors, Proteins, Nanoparticles, Metals, Ions, Biosensors, Quantum dots, Absorbance, Analytical research, Neodymium

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PROCEEDINGS ARTICLE | March 12, 2015

Membrane-targeting peptides for nanoparticle-facilitated cellular imaging and analysis

Proc. SPIE. 9338, Colloidal Nanoparticles for Biomedical Applications X

KEYWORDS: Nanoparticles, Metals, Luminescence, Zinc, Quantum dots, Nanomaterials, Physiology, Biological research, Analytical research, Plasma

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PROCEEDINGS ARTICLE | March 10, 2015

Visualization and neuronal cell targeting during electrophysiological recordings facilitated by quantum dots

Proc. SPIE. 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II

KEYWORDS: Visualization, Tissues, Coating, Quantum dots, Physiology, In vivo imaging, Thin film coatings, Plasma, Neurons, Brain

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PROCEEDINGS ARTICLE | August 27, 2014

Controlling the intracellular fate of nano-bioconjugates: pathways for realizing nanoparticle-mediated theranostics

Proc. SPIE. 9166, Biosensing and Nanomedicine VII

KEYWORDS: Gold, Proteins, Imaging systems, Nanoparticles, Quantum dots, Toxicity, Nanomaterials, Analytical research, Neptunium, Plasma

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PROCEEDINGS ARTICLE | May 28, 2014

FRET based characterization of DNA-based assemblies

Proc. SPIE. 9107, Smart Biomedical and Physiological Sensor Technology XI

KEYWORDS: Nanostructures, Mica, Spectroscopy, Luminescence, Fluorescence spectroscopy, Atomic force microscopy, Chromophores, Distance measurement, Fluorescence resonance energy transfer, Anisotropy

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PROCEEDINGS ARTICLE | May 22, 2014

Monitoring enzyme kinetic behavior of enzyme-quantum dot bioconjugates

Proc. SPIE. 9107, Smart Biomedical and Physiological Sensor Technology XI

KEYWORDS: Semiconductors, Biosensing, Nanoparticles, Metals, Quantum efficiency, Resistance, Quantum dots, In vivo imaging, Nanocrystals, Molecular photonics

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PROCEEDINGS ARTICLE | October 1, 2013

Enhancing molecular logic through modulation of temporal and spatial constraints with quantum dot-based systems that use fluorescent (Förster) resonance energy transfer

Proc. SPIE. 8817, Nanobiosystems: Processing, Characterization, and Applications VI

KEYWORDS: Logic, Modulation, Ultraviolet radiation, Luminescence, Quantum dots, Terbium, Resonance energy transfer, Fluorescence resonance energy transfer, Resonance enhancement, Molecular energy transfer

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PROCEEDINGS ARTICLE | October 1, 2013

Improving energy transfer in QD-DNA photonic networks

Proc. SPIE. 8817, Nanobiosystems: Processing, Characterization, and Applications VI

KEYWORDS: Nanostructures, Energy transfer, Chemistry, Quantum dots, Chromophores, Resonance energy transfer, Excitons, Thulium, Analytical research, Fluorescence resonance energy transfer

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PROCEEDINGS ARTICLE | May 29, 2013

Peptide-mediated cellular delivery of semiconductor quantum dots

Proc. SPIE. 8723, Sensing Technologies for Global Health, Military Medicine, and Environmental Monitoring III

KEYWORDS: Semiconductors, Mirrors, Proteins, Biosensing, Nanoparticles, Image analysis, Quantum dots, Toxicity, Fluorescent proteins, In vitro testing

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PROCEEDINGS ARTICLE | February 22, 2013

Fluorescent nanocolloids for differential labeling of the endocytic pathway and drug delivery applications

Proc. SPIE. 8595, Colloidal Nanocrystals for Biomedical Applications VIII

KEYWORDS: Proteins, Iron, Biomedical optics, Tissues, Nanoparticles, Control systems, Quantum dots, Liquid crystals, Nanomaterials, Target recognition

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PROCEEDINGS ARTICLE | February 22, 2013

Recent development of dihydrolipoic acid appended ligands for robust and biocompatible quantum dots

Proc. SPIE. 8595, Colloidal Nanocrystals for Biomedical Applications VIII

KEYWORDS: Semiconductors, Proteins, Biosensing, Nanoparticles, Luminescence, Quantum efficiency, Coating, Zinc, Quantum dots, Adsorption

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PROCEEDINGS ARTICLE | February 2, 2012

Further progress in cytosolic cellular delivery of quantum dots

Proc. SPIE. 8232, Colloidal Nanocrystals for Biomedical Applications VII

KEYWORDS: Proteins, Biosensing, Nanoparticles, Polymers, Luminescence, Manufacturing, Zinc, Quantum dots, Toxicity, In vivo imaging

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PROCEEDINGS ARTICLE | February 12, 2011

Quantum dots as a FRET donor and nanoscaffold for multivalent DNA photonic wires

Proc. SPIE. 7909, Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI

KEYWORDS: Energy efficiency, Energy transfer, Composites, Quantum efficiency, Quantum dots, Chromophores, Fluorescence resonance energy transfer, Quenching (fluorescence), DNA photonics, Absorption

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PROCEEDINGS ARTICLE | May 5, 2010

Quantum dots in life sciences: applications, benefits, and safety issues

Proc. SPIE. 7666, Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX

KEYWORDS: Semiconductors, Safety, Polymers, Spectroscopy, Quantum efficiency, Quantum dots, Life sciences, Biology, Optical spectroscopy, Analytical research

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PROCEEDINGS ARTICLE | February 18, 2010

Delivery of quantum dot bioconjugates to the cellular cytosol: release from the endolysosomal system

Proc. SPIE. 7575, Colloidal Quantum Dots for Biomedical Applications V

KEYWORDS: Semiconductors, Proteins, Tissues, Polymers, Quantum dots, Toxicity, Cell biology, Analytical research, Sodium, Plasma

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To realize their full potential as intracellular imaging and sensing reagents, robust and efficient methods for the targeted cellular delivery of luminescent semiconductor quantum dots (QDs) must be developed. We have previously shown that QDs decorated with histidine-terminated polyarginine cell-penetrating peptides (CPP) are rapidly and specifically internalized via endocytosis by several mammalian cell lines with no cytotoxicity. Here we demonstrate the long-term intracellular stability and fate of these QD-peptide conjugates. We found that the QD-peptide conjugates remain sequestered within endolysosomal vesicles for up to three days after delivery. However, the CPP appeared to remain stably associated with the QD within these acidic vesicles over this time period. Hence, we explored a number of techniques to either actively deliver QDs directly to the cytosol or to facilitate the endosomal release of endocytosed QDs to the cytosol. Active methods (e.g., electroporation) delivered only modest amounts of QDs to the cytosol that appeared to form aggregates. Delivery of QDs using polymer-based transfection reagents resulted primarily in the endosomal sequestration of the QDs, although one commercial polymer tested delivered QDs to the cytosol but only after several days in culture and with a considerable degree of polymer-induced toxicity. Finally, a modular, amphiphilic peptide containing functionalities designed for cell penetration and vesicular membrane interaction demonstrated the ability to deliver QDs in a well-dispersed manner to the cytosol. This peptide mediated rapid QD uptake followed by a slower efficient endosomal release of the QDs to the cytosol that peaked at 48 hours post-delivery. Importantly, this QD-peptide conjugate elicited minimal cytotoxicity in two cell lines tested. A more detailed understanding of the mechanism of the peptide's uptake and endosomal escape attributes will lead to the design of further QD conjugates for targeted imaging and sensing applications.

PROCEEDINGS ARTICLE | February 18, 2010

Optical size determination of quantum dots using FRET with terbium complexes as donors

Proc. SPIE. 7575, Colloidal Quantum Dots for Biomedical Applications V

KEYWORDS: Semiconductors, Spectroscopy, Luminescence, Multiplexing, Quantum dots, Terbium, Fluorescence resonance energy transfer, Signal detection, Single molecule spectroscopy, Absorption

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PROCEEDINGS ARTICLE | March 3, 2009

Peptide linkers for the assembly of semiconductor quantum dot bioconjugates

Proc. SPIE. 7189, Colloidal Quantum Dots for Biomedical Applications IV

KEYWORDS: Semiconductors, Gold, Proteins, Quantum efficiency, Quantum dots, Distance measurement, Fluorescent proteins, Fluorescence resonance energy transfer, Nanolithography, In vitro testing

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PROCEEDINGS ARTICLE | March 3, 2009

Intracellular delivery of and sensing with quantum dot bioconjugates

Proc. SPIE. 7189, Colloidal Quantum Dots for Biomedical Applications IV

KEYWORDS: Semiconductors, Polymers, Luminescence, Quantum efficiency, Multiplexing, Quantum dots, Toxicity, Receptors, Analytical research, Absorption

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PROCEEDINGS ARTICLE | March 3, 2009

Multivalent display of DNA conjugates on semiconductor quantum dots utilizing a novel conjugation method

Proc. SPIE. 7189, Colloidal Quantum Dots for Biomedical Applications IV

KEYWORDS: Semiconductors, Biomedical optics, Sensors, Nanoparticles, Particles, Chemistry, Quantum dots, In vivo imaging, Nanocrystals, Atmospheric particles

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PROCEEDINGS ARTICLE | March 3, 2009

Using metal complex-labeled peptides for charge transfer-based biosensing with semiconductor quantum dots

Proc. SPIE. 7189, Colloidal Quantum Dots for Biomedical Applications IV

KEYWORDS: Semiconductors, Biosensing, Metals, Luminescence, Quantum dots, Nanocrystals, Environmental sensing, Ruthenium, Absorption, Oxidation

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PROCEEDINGS ARTICLE | February 19, 2009

Energy transfer-based biosensing of protease activity measured using an electroluminescent platform

Proc. SPIE. 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems

KEYWORDS: Semiconductors, Biosensing, Medicine, Sensors, Luminescence, Electroluminescence, Quantum dots, Charge-coupled devices, Fluorescence resonance energy transfer, Bandpass filters

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PROCEEDINGS ARTICLE | April 15, 2008

Self-assembled quantum dot-bioconjugates: characterization and use for sensing proteolytic activity

Proc. SPIE. 6945, Optics and Photonics in Global Homeland Security IV

KEYWORDS: Semiconductors, Proteins, Biosensing, Luminescence, Energy transfer, Chemistry, Quantum dots, Biology, Fluorescence resonance energy transfer, Nanocrystals

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PROCEEDINGS ARTICLE | March 13, 2008

Assembly and intracellular delivery of quantum dot-fluorescent protein bioconjugates

Proc. SPIE. 6866, Colloidal Quantum Dots for Biomedical Applications III

KEYWORDS: Proteins, Sensors, Nanoparticles, Luminescence, Quantum dots, Digital image correlation, Fluorescent proteins, Fluorescence resonance energy transfer, Nanocrystals, Nanolithography

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PROCEEDINGS ARTICLE | February 26, 2008

Specific cellular delivery and intracellular fate of quantum dot-peptide and quantum dot-polymer nanoassemblies

Proc. SPIE. 6866, Colloidal Quantum Dots for Biomedical Applications III

KEYWORDS: Semiconductors, Biomedical optics, Polymers, Metals, Spectroscopy, Luminescence, Ions, Quantum dots, Toxicity, Biology

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PROCEEDINGS ARTICLE | February 22, 2008

New ligand design to promote water compatibility of luminescent quantum dots and gold nanoparticles

Proc. SPIE. 6866, Colloidal Quantum Dots for Biomedical Applications III

KEYWORDS: Gold, Silica, Optical properties, Nanoparticles, Luminescence, Nitrogen, Quantum dots, Sodium, Nanocrystals, Absorption

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PROCEEDINGS ARTICLE | February 27, 2007

Single quantum dot fluorescence resonant energy transfer: probing the heterogeneity in macroscopic samples

Proc. SPIE. 6448, Colloidal Quantum Dots for Biomedical Applications II

KEYWORDS: Energy efficiency, Proteins, Particles, Luminescence, Molecules, Energy transfer, Quantum dots, Resonance energy transfer, Fluorescence resonance energy transfer, Absorption

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PROCEEDINGS ARTICLE | February 27, 2007

Selective cellular delivery of self-assembled quantum dot-peptide bioconjugates

Proc. SPIE. 6448, Colloidal Quantum Dots for Biomedical Applications II

KEYWORDS: Semiconductors, Luminescence, Quantum efficiency, Control systems, Quantum dots, Toxicity, Analytical research, Sodium, Signal detection, Absorption

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PROCEEDINGS ARTICLE | February 27, 2007

Quantum dot-DNA bioconjugates for fluorescence-resonance-energy-transfer-based biosensing

Proc. SPIE. 6448, Colloidal Quantum Dots for Biomedical Applications II

KEYWORDS: Semiconductors, Proteins, Biosensing, Luminescence, Quantum efficiency, Quantum dots, Resonance energy transfer, Analytical research, Fluorescence resonance energy transfer, Absorption

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PROCEEDINGS ARTICLE | March 27, 2006

Hydrodynamic sizes of functional hydrophilic QDs

Proc. SPIE. 6096, Colloidal Quantum Dots for Biomedical Applications

KEYWORDS: Proteins, Nanoparticles, Polymers, Particles, Diffusion, Coating, Quantum dots, Dynamic light scattering, Nanocrystals, Signal detection

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PROCEEDINGS ARTICLE | March 27, 2006

Quantum-dot-based nanosensors designed for proteolytic monitoring

Proc. SPIE. 6096, Colloidal Quantum Dots for Biomedical Applications

KEYWORDS: Nanosensors, Proteins, Sensors, Luminescence, Quantum dots, Explosives, Biological research, Fluorescence resonance energy transfer, Nanocrystals, Absorption

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PROCEEDINGS ARTICLE | March 27, 2006

Quantum dot-based fluorescence resonance energy transfer multiplexing: applications for biosensing

Proc. SPIE. 6096, Colloidal Quantum Dots for Biomedical Applications

KEYWORDS: Nanosensors, Proteins, Biosensing, Luminescence, Composites, Multiplexing, Quantum dots, Deconvolution, Fluorescence resonance energy transfer, Absorption

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PROCEEDINGS ARTICLE | March 27, 2006

Probing the effects of spectral overlap on quantum-dot-based FRET: Ensemble and single molecule studies

Proc. SPIE. 6096, Colloidal Quantum Dots for Biomedical Applications

KEYWORDS: Semiconductors, Proteins, Luminescence, Molecules, Energy transfer, Quantum dots, Resonance energy transfer, Fluorescence resonance energy transfer, Nanocrystals, Absorption

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SPIE Conference Volume | February 10, 2006

Nanobiophotonics and Biomedical Applications III

PROCEEDINGS ARTICLE | April 28, 2005

Are luminescent quantum dots efficient energy acceptors?

Proc. SPIE. 5705, Nanobiophotonics and Biomedical Applications II

KEYWORDS: Proteins, Luminescence, Molecules, Energy transfer, Quantum efficiency, Quantum dots, Excitons, Time resolved spectroscopy, Fluorescence resonance energy transfer, Absorption

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PROCEEDINGS ARTICLE | April 28, 2005

Synthesis of surface ligands to prepare hydrophilic and biologically compatible quantum dots

Proc. SPIE. 5705, Nanobiophotonics and Biomedical Applications II

KEYWORDS: Oxides, Magnesium, Nanoparticles, Luminescence, Molecules, Resistance, Bioalcohols, Quantum dots, Nanocrystals, Natural surfaces

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PROCEEDINGS ARTICLE | April 28, 2005

A fluorescence resonance energy transfer quantum dot explosive nanosensor

Proc. SPIE. 5705, Nanobiophotonics and Biomedical Applications II

KEYWORDS: Nanosensors, Proteins, Sensors, Luminescence, Biosensors, Quantum dots, Explosives, Fluorescence resonance energy transfer, Chemical elements, Analog electronics

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PROCEEDINGS ARTICLE | April 4, 2005

Quantum-dot-based multiplexed fluorescence resonance energy transfer

Proc. SPIE. 5704, Genetically Engineered and Optical Probes for Biomedical Applications III

KEYWORDS: Proteins, Luminescence, Composites, Biosensors, Multiplexing, Quantum dots, Deconvolution, Fluorescence resonance energy transfer, Signal detection, Absorption

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PROCEEDINGS ARTICLE | December 29, 2004

Modulation of quantum dot photoemission based on fluorescence resonance energy transfer to a photochromic dye acceptor

Proc. SPIE. 5593, Nanosensing: Materials and Devices

KEYWORDS: Surface plasmons, Proteins, Switching, Modulation, Ultraviolet radiation, Luminescence, Molecules, Quantum dots, Fluorescence resonance energy transfer, Absorption

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