A new analytical method of high-selective detection of mycotoxins in food and feed are considered. A method is based
on optical registration the changes of conduct of the electric polarized bacterial agents in solution at the action of the
external gradient electric fields. Measuring are conducted in integrated electrode-optical cuvette of the special
construction, which provides the photometric analysis of forward motion of the objects registration in liquid solution
under act of the enclosed electric field and simultaneous registration of kinetics of change of electrical impedance
parameters solution and electrode system.
Device for registration of bacterial cells it is possible to examine as an information task which is characterized by a priori
vagueness of moment of appearance of prototype system in the area of their registration. These reasons do not allow to
provide exactness measuring on the base of measuring charts, functioning of which is provided structurally, and require
the program hardware methods of measuring. An effective decision of this task is possible subject to condition
application of the adaptive, program-driven methods, realized on the base of the fast-acting specialized charts of high
integration. A new device for registration of bacterial cells in liquid medium after the changes of them size distributing is
described. Procedure of registration of bacterial cells consists in dissociating of signal from noises, amplitude and
duration measurement of the proper electric impulses. The process of registration particles in real-time imaging the
screening on monitor of the personal computer. The developed software allows estimating the size distributing of the
explored objects and their concentration in a liquid medium in 3D-imaging. There possibilities of device from
registration and visualization of bacterial cells of different nature are presented.
Method of rapid detection of bacterial cells by light scattering is described. Determination of quantitative changes of
bacteria is the given method based on the changes of their size distributing in the process of cultivation. Liquid medial
are diluted and analyzed by the proposed technology to determine presence of bacteria. A method includes sounding of
flow suspended bacterial cells by monochromatic coherent light, registration of signals of co-operation of sounding
radiation with the explored microbiological objects by detects amplitudes and durations of scattered light impulses.
Distribution of particles by sizes is determined from the measured functional dependence of number of registered
particles from amplitude and duration of the proper electric impulses on the output photoreceiver. Detection is done for a
range of particle size from 0.1 to 10 mkm, and thus particle's size distribution is determined. The results of studying of
rapid detection of Escherichia coli by light scattering are described.
Monitoring of bacterial cell numbers is of great importance not only in microbiological industry but also for control of
liquids contamination in the food and pharmaceutical industries. Here we describe a novel low-cost and highly efficient
technology for bacterial cell monitoring during cultivation process. The technology incorporates previously developed
monitoring device and algorithm of its action. The devise analyses light scattered by suspended bacterial cells. Current
stage utilizes monochromatic coherent light and detects amplitudes and durations of scattered light impulses, it does not
require any labeling of bacterial cell. The system is calibrated using highly purificated bacteria-free water as standard.
Liquid medial are diluted and analyzed by the proposed technology to determine presence of bacteria. Detection is done
for a range of particle size from 0.1 to 10 μm, and thus particles size distribution is determined. We analyzed a set of
different bacterial suspensions and also their changes in quantity and size distribution during cultivation. Based on the
obtained results we conclude that proposed technology can be very effective for bacteria monitoring during cultivation
process, providing benefits of low simplicity and low cost of analysis with simultaneous high detection precision.