Various techniques for recovering optical parameters were developed over the years. However each has its limitations, constraints and disadvantages (e.g. accuracy, computational speed, sample assembly, distinguishing between the different parameters, etc.). This research suggests an optical technique for extracting the reduced scattering coefficient (μs') of substances by examining the light transmission through or reflection from them. It uses the multiple planes Gerchberg- Saxton (G-S) algorithm to reconstruct the light phase created by the substance. At the end of the algorithm, μs' can be estimated from the standard deviation (STD) of the retrieved phase of the reemitted light. We will use the theory to compute the phase’s STD that directly correlated to the optical properties of different substances. Two possible applications for this technique, out of many others, are nanoparticles (NPs) penetration depth determination, for promoting topical medications, and detection of milk components quantitative signature as en route to milk content monitoring tool. For the former application, three materials were fabricated into NPs and all presented an activity enhancement with their size reduction. Then the NPs were applied on tissues and detected by our technique. For the latter, different milk content concentrations were examined resulting with different STD values suggesting it can be used as indicator for the milk component concentrations.
In recent years, infiltrating materials into the human body has become a great challenge many researches are facing. In medicine and cosmetics today, there are materials which are administrated to patients by injection only. The main challenge with topical medication is penetrating the skin barrier. The skin is an effective barrier between the body and the outside environment, which prevents foreign materials entering the body easily. However, reducing the size of the desired materials might help their skin penetration ability. Recently nanoparticles (NPs) are being evaluated for use in many fields like chemistry, biology, medicine, physics and optics. The technique used in this work for forming organic NPs (ONPs) is the application of sonic waves to an aqueous solution, known as sonochemistry. To investigate the physical penetration depth of ONPs into the human body, we first developed a novel optical technique for detecting NPs within tissues. The detection of NPs is done by the extraction and investigation of the reemitted light phase.