Prof. Jacques Derouard Profile

Prof. Jacques Derouard

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Publications (8)

Proceedings Article | 7 April 2020 Presentation

Fluorescence speckle image correlation spectroscopy (Conference Presentation)

Anirban Sarkar, Irène Wang, Aditya Katti, Jörg Enderlein, Jacques Derouard, Antoine Delon

Proceedings Volume 11351, 1135108 (2020) https://doi.org/10.1117/12.2558129

KEYWORDS: Speckle, Scattering, Fluorescence correlation spectroscopy, Spectroscopy, Light scattering, Luminescence, Fluorescence spectroscopy, Imaging spectroscopy, Particles, Speckle pattern

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Fluorescence correlation spectroscopy is used extensively for quantitative characterization of biomolecules at very low concentration. However, light aberration and scattering from the tissues are two major factors that affect the results strongly. Although adaptive optics arrangement can correct the aberrations of light to some extent, it fails completely to eliminate the light scattering effect. Recently, exploiting the fact that autocorrelation of a speckle pattern is a sharply peaked point spread function and the optical memory effect, non-invasive imaging of fluorescent sample through a scattering medium has been possible. However, it is also very challenging to measure the dynamic properties of the fluorescent molecules or particles through a scattering layer due to poor signal to noise ratio. In this study, we employ a modality based on speckle cross-correlation enabled via optical memory effect to study two dimensional (2D) diffusion of fluorescent particles hidden behind a scattering film. We realized a 2D diffusing model system by confining fluorescent polystyrene beads of 1µm diameter at the water/air interface behind a TiO2 diffuser. The experimental set up was built up in an epifluorescence configuration. The fluorescent beads were excited by an illumination speckle generated by the incident light in a plane-wave geometry while passing through the disordered TiO2 film. Similarly, the emitted fluorescent signal also traversed through the same TiO2 film to generate the detection speckle, which was eventually recorded by a high frame rate CMOS camera. The experimental set up has also been modelled numerically, where speckle pattern has been generated by a spherical wave, transmitted through a scattering object in an optical microscope. Moreover, the dependence of the speckle size on the numerical aperture, magnification, and the distance of the focal plane from the bead plane has also been studied. The numerical results have been compared with the experimental values to estimate the speckle size. Furthermore, we have evaluated the 2D diffusion constant by monitoring the widening of the 2D speckle cross-correlation function versus lag time. This result has been compared with that obtained with the single particle tracking method without the scattering layer. Quantitative agreement between the results obtained by the speckle cross-correlations and the single particle tracking technique without the diffuser establishes the potential application of this technique in correlation spectroscopy. Superimposed multiple beads speckle patterns were also studied and the results will be presented in the conference.

Proceedings Article | 28 July 2017 Paper

Performance evaluation of time-domain multispectral diffuse optical tomography in the reflection geometry

Lionel Hervé, Judy Zouaoui, David Orive-Miguel, Laura Di Sieno, Antonio Pifferi, Andrea Farina, Alberto Dalla Mora, Jacques Derouard, Jérôme Mars, Laurent Condat, Jean-Marc Dinten

Proceedings Volume 10412, 1041208 (2017) https://doi.org/10.1117/12.2285811

KEYWORDS: Diffuse optical tomography, Tomography, Image restoration, Photon counting, Photodetectors

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Proceedings Article | 16 July 2015 Paper

In-depth quantification by using multispectral time-resolved diffuse optical tomography

Judy Zouaoui, Lionel Hervé, Laura Di Sieno, Anne Planat-Chrétien, Michel Berger, Alberto Dalla Mora, Antonio Pifferi, Jacques Derouard, Jean-Marc Dinten

Proceedings Volume 9538, 95380C (2015) https://doi.org/10.1117/12.2183819

KEYWORDS: Chromophores, Diffuse optical tomography, Absorption, Photons, Optical fibers, Tissues, Sensors, Transform theory, Light, Liquids

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Proceedings Article | 9 February 2007 Paper

Optical manipulation of gold micro and nano-particles on silicon nitride waveguides: impact of polarization and particle size on gradient forces

Jean-Marc Fedeli, Stéphanie Gaugiran, Jacques Derouard, Stéphane Gétin

Proceedings Volume 6483, 64830E (2007) https://doi.org/10.1117/12.700635

KEYWORDS: Particles, Waveguides, Dielectric polarization, Gold, Silicon, Polarization, Electromagnetism, Glasses, Wave propagation, Radio propagation

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Proceedings Article | 14 April 2006 Paper

Spatial fluorescence cross-correlation spectroscopy between core and ring pinholes

Rodolphe Jaffiol, Jacques Derouard, Yoann Blancquaert, Antoine Delon

Proceedings Volume 6191, 61910B (2006) https://doi.org/10.1117/12.662244

KEYWORDS: Diffusion, Luminescence, Fluorescence correlation spectroscopy, Bioalcohols, Fluorescence spectroscopy, Spectroscopy, Molecules, Confocal microscopy, Optical fibers, Water

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Proceedings Article | 14 April 2006 Paper

Application of fluorescence fluctuation spectroscopy to the measurement of the concentration of molecules deposited on solid substrates

Jacques Derouard, Antoine Delon, Rodolphe Jaffiol, Cyrille Vézy

Proceedings Volume 6191, 61910C (2006) https://doi.org/10.1117/12.662294

KEYWORDS: Luminescence, Molecules, Solids, Photon counting, Glasses, Statistical analysis, Molecular spectroscopy, Error analysis, Confocal microscopy, Particles

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Proceedings Article | 31 October 2001 Paper

Noninvasive optical monitoring of rat brain and effects of the injection of tracers for blood flow measurements

E. Grillon, Raphael Sablong, Jacques Derouard, Oliver Hugon

Proceedings Volume 4432, (2001) https://doi.org/10.1117/12.447142

KEYWORDS: Brain, Magnetism, Near infrared spectroscopy, Heart, Electrocardiography, Absorption, Data acquisition, Linear filtering, Blood circulation, Indocyanine green

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Proceedings Article | 31 October 2001 Paper

In-vivo absorption spectroscopy in brain using small optical fiber probes: effect of blood confinement

C. Julien, Raphael Sablong, Adrian Bradu, Jacques Derouard, I. Tropres, J. Payen

Proceedings Volume 4432, (2001) https://doi.org/10.1117/12.447121

KEYWORDS: Signal attenuation, Blood, Absorption, Absorption spectroscopy, Tissues, Optical fibers, Brain, In vivo imaging, Reflectivity, Magnetism

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Showing 5 of 8 publications

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