J. Spigulis graduated as physicist from the University of Latvia in 1973. PhD thesis on optical processes in metal vapors have been defended in 1979, and Dr.Habil. Phys. thesis on novel optoelectronic methods and devices - in 1993. SInce 1986 his research is focused to fiberoptics, optoelectronics and biomedical optics; he established and leads the Bio-optics and Fibre-optics Laboratory at the Institute of Atomic Physics and Spectroscopy, University of Latvia (currently being Director of the Institute). His work has been related to design and investigation of optical fibre sensors, communication devices, medical lightguide systems and new types of the side-glowing optical fibres, as well as to development of novel optical methods for nonivasive clinical diagnostics and monitoring. Dr. Spigulis has developed lecture courses "Lightguide Physics", "Optoelectronics", “Laser Physics” and “Earth Physics” for BSc. students, as well as "Fundamentals of Biomedical Optics" and "Medical Lightguides" for MSc. students. In 1995 he launched the MSc program on Biomedical Optics at University of Latvia and actively co-ordinated this program over the following years.
J. Spigulis was the founder, chairman and co-chairman of the Baltic Chapter of SPIE – the International Society for Optical Engineering, he is a member of SPIE, Optical Society of America (OSA), Latvian Union of Scientists, Latvian Physical Society and Latvian Society of Medical Engineering and Physics. Currently he is presiding Latvian Regional Committee of ICO – International Commission for Optics, and the Latvian Local Section of OSA. In 2007 J. Spigulis became the Corresponding member of Latvian Academy of Sciences.
J. Spigulis was the founder, chairman and co-chairman of the Baltic Chapter of SPIE – the International Society for Optical Engineering, he is a member of SPIE, Optical Society of America (OSA), Latvian Union of Scientists, Latvian Physical Society and Latvian Society of Medical Engineering and Physics. Currently he is presiding Latvian Regional Committee of ICO – International Commission for Optics, and the Latvian Local Section of OSA. In 2007 J. Spigulis became the Corresponding member of Latvian Academy of Sciences.
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Skin chromophore mapping by smartphone RGB camera under spectral band and spectral line illumination
It is important to develop and combine novel diagnostic techniques for an accurate early stage diagnosis to improve the chances for skin and GIT tumours treatment. Optical techniques are very promising methods for such noninvasive diagnosis of skin and mucosa tumours, possessing the advantages of deep imaging depth, high resolution, fast imaging speed, and noninvasive character of detection. In this study we combine autofluorescence spectroscopy and optical imaging techniques to develop more precise evaluation of the tissue pathologies investigated.
We obtain chromophore maps for GIT and cutaneous samples, with better visualization of the tumours borders and margins. In addition, fluorescence spectra give us information about the early changes in chromophores’ contents into the tissues during neoplasia growth.
The multispectral imaging system Nuance based on liquid crystal tunable filters was adapted for diffuse reflectance and fluorescence spectral imaging of in vivo skin. Uniform illumination was achieved by LED ring light. Combination of four LEDs (warm white, 770 nm, 830 nm and 890 nm) was used to support diffuse reflectance mode in spectral range 450-950 nm. 405 nm LEDs were used for excitation of skin autofluorescence. Multispectral imaging system was adapted for spectral working range of 450-950 nm with scanning step of 10 nm and spectral resolution of 15 nm. An average field of view was 50x35 mm in size with spatial resolution 0,05 mm (the pixel size). Due to spectrally different illumination intensity and system sensitivity, various exposure times (from 7…500 ms) were used for each image acquisition.
The proposed approach was tested for different skin lesions: benign nevus, hemangioma, basalioma and halo nevus. Spectral image cubes of different skin lesions were acquired and analyzed to test its diagnostic potential.
In this work we demonstrate simple implementation of LASCA imaging technique as connection kit for mobile phone for primary low-cost assessment of skin blood flow. Stabilized 650 nm and 532 nm laser diode modules were used for LASCA illumination. Dual wavelength illumination could provide additional information about skin hemoglobin and oxygenation level.
The proposed approach was tested for arterial occlusion and heat test. Besides, blood flow maps of injured and provoked skin were demonstrated.
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