Ms. Jasmin M. Schaefer Profile

Jasmin M. Schaefer

at Lumencor Inc

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Author

Publications (3)

Proceedings Article | 7 March 2019 Paper

Diagnostic performance of receptor-specific surgical specimen staining correlate with receptor expression level

Broderick House, Jasmin Schaefer, Connor Barth, Scott Davis, Summer Gibbs

Proceedings Volume 10862, 108620K (2019) https://doi.org/10.1117/12.2510625

KEYWORDS: Tumors, Tissues, Surgery, Cancer, Diagnostics, Breast, Receptors, Breast cancer, Luminescence

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Identification of tumor margins in the operating room in real time is a critical challenge for surgical procedures that serve as cancer cure. Breast conserving surgery (BCS) is particularly affected by this problem, with current reexcision rates above 25%. Due to a lack of clinically available methodologies for detection of involved or close tumor margins, much effort is focused on developing intraoperative margin assessment modalities that can aid in addressing this unmet clinical need. BCS provides a unique opportunity to design contrast-based technologies that are able to assess tumor margins independent from the patient, providing a rapid pathway from bench to bedside at a much lower cost. Since resected tissue is removed from the patient’s blood supply, non-specific contrast agent uptake becomes a challenge due to the lack of clearance. Therefore, a dual probe, ratiometric fluorescence imaging approach was taken in an effort to reduce non-specific signal, and provide a modality that could demonstrate rapid, robust margin assessment on resected patient samples. Termed, dual-stain difference specimen imaging (DDSI), DDSI includes the use of spectrally unique, and fluorescently labeled target-specific, as well as non-specific biomarkers. In the present study, we have applied epidermal growth factor receptor (EGFR) targeted DDSI to tumor xenografts with variable EGFR expression levels using a previously optimized staining protocol, allowing for a quantitative assessment of the predictive power of the technique under biologically relevant conditions. Due to the presence of necrosis in the model tumors, ring analysis was employed to characterize diagnostic accuracy as measured by receiver operator characteristic (ROC) curve analysis. Our findings demonstrate the robust nature of the DDSI technique even in the presence of variable biomarker expression and spatial patterns. These results support the continued development of this technology as a robust diagnostic tool for tumor margin assessment in resected specimens during BCS.

SPIE Journal Paper | 8 February 2019 Open Access

Diagnostic performance of receptor-specific surgical specimen staining correlates with receptor expression level

Jasmin Schaefer, Connor Barth, Scott Davis, Summer Gibbs

JBO, Vol. 24, Issue 02, 026002, (February 2019) https://doi.org/10.1117/12.10.1117/1.JBO.24.2.026002

KEYWORDS: Tumors, Tissues, Diagnostics, Receptors, Luminescence, Surgery, Biological research, Cancer, Flow cytometry, Statistical analysis

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Proceedings Article | 14 March 2018 Presentation

Dual probe difference specimen imaging for rapid identification of margin assessment during breast conserving surgery (Conference Presentation)

Jasmin Schaefer, Connor Barth, Vince Rossi, Kimberley Samkoe, Scott Davis, Summer Gibbs

Proceedings Volume 10478, 104780M (2018) https://doi.org/10.1117/12.2290677

KEYWORDS: Surgery, Breast cancer, Tumors, Luminescence, Breast, Image-guided intervention, Molecules, Tissues

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