Prof. Ji-Xin Cheng Profile

Prof. Ji-Xin Cheng

at Boston Univ

SPIE Involvement:

Conference Program Committee | Author

Publications (29)

SPIE Journal Paper | October 22, 2018

Imaging of demineralized enamel in intact tooth by epidetected stimulated Raman scattering microscopy

Masatoshi Ando, Chien-Sheng Liao, George J. Eckert, Ji-Xin Cheng

JBO Vol. 23 Issue 10

KEYWORDS: Dental caries, Carbonates, Teeth, Raman spectroscopy, Minerals, Microscopy, Water, Raman scattering, Crystals, Chemical analysis

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Stimulated Raman scattering microscopy (SRS) was deployed to quantify enamel demineralization in intact teeth. The surfaces of 15 bovine-enamel blocks were divided into four equal-areas, and chemically demineralized for 0, 8, 16, or 24 h, respectively. SRS images (spectral coverage from ∼850 to 1150 cm − 1) were obtained at 10-μm increments up to 90 μm from the surface to the dentin–enamel junction. SRS intensities of phosphate (peak: 959 cm − 1), carbonate (1070 cm − 1), and water (3250 cm − 1) were measured. The phosphate peak height was divided by the carbonate peak height to calculate the SRS-P/C-ratio, which was normalized relative to 90 μm (SRS-P/C-ratio-normalized). The water intensity against depth decay curve was fitted with exponential decay. A decay constant (SRS-water-content) was obtained. Knoop-hardness values were obtained before (SMHS) and after demineralization (SMHD). Surface microhardness-change (SMH-change) [ ( SMHD − SMHS ) / SMHS] was calculated. Depth and integrated mineral loss (ΔZ) were determined by transverse microradiography. Comparisons were made using repeated-measures of analysis of variance. For SRS-P/C-ratio-normalized, at 0-μm (surface), sound (0-h demineralization) was significantly higher than 8-h demineralization and 24-h demineralization; 16-h demineralization was significantly higher than 24-h demineralization. For SRS-water-content, 24-h demineralization was significantly higher than all other demineralization-groups; 8-h demineralization and 16-h demineralization were significantly higher than 0-h demineralization. SRS-water-content presented moderate-to-strong correlation with SMH-change and weak-to-moderate correlation with depth. These results collectively demonstrate the potential of using SRS microscopy for in-situ chemical analysis of dental caries.

PROCEEDINGS ARTICLE | March 14, 2018

In vivo and in situ spectroscopic imaging by a handheld stimulated Raman microscope (Conference Presentation)

Proc. SPIE. 10489, Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis

KEYWORDS: Optical fibers, Microscopes, Tissues, Spectroscopy, Microscopy, Imaging spectroscopy, Raman spectroscopy, Raman scattering, In vivo imaging, Signal detection

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PROCEEDINGS ARTICLE | February 8, 2018

Staphyloxanthin photobleaching sensitizes methicillin-resistant Staphylococcus aureus to reactive oxygen species attack

Proc. SPIE. 10479, Light-Based Diagnosis and Treatment of Infectious Diseases

KEYWORDS: Microscopes, Light emitting diodes, Antimicrobial agents, Error analysis, Hydrogen, Resistance, Mass spectrometry, Oxygen, Chromophores, Photonics, Microbiology, Photomedicine, Biophysics, Absorption

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PROCEEDINGS ARTICLE | April 24, 2017

Pushing the physical limits of spectroscopic imaging for new biology and better medicine (Conference Presentation)

Proc. SPIE. 10076, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II

KEYWORDS: Mid-IR, Microscopes, Medicine, Cancer, Spectroscopy, Imaging spectroscopy, Biology, Molecular spectroscopy, Stem cells, In vivo imaging

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PROCEEDINGS ARTICLE | April 24, 2017

Aberrant lipogenesis is a metabolic marker for azole-resistant candida albicans (Conference Presentation)

Proc. SPIE. 10069, Multiphoton Microscopy in the Biomedical Sciences XVII

KEYWORDS: Microscopes, Multiphoton microscopy, Biomedical optics, Diagnostics, Microorganisms, Pathogens, Raman scattering, Current controlled current source, Mode conditioning cables

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PROCEEDINGS ARTICLE | April 19, 2017

An optoacoustic guide with augmented reality system towards precision breast conserving surgery (Conference Presentation)

Proc. SPIE. 10054, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XV

KEYWORDS: Radar, Breast, Tumors, Visualization, Surgery, Augmented reality, Acoustics, Biomedical engineering, Autoregressive models, Light

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PROCEEDINGS ARTICLE | March 3, 2017

Real-time intravascular photoacoustic-ultrasound imaging of lipid-laden plaque at speed of video-rate level

Proc. SPIE. 10064, Photons Plus Ultrasound: Imaging and Sensing 2017

KEYWORDS: Mirrors, Real time imaging, Fiber optics, Imaging systems, Ultrasonography, Image processing, Image resolution, Photoacoustic imaging, In vivo imaging, High speed imaging, Absorption

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PROCEEDINGS ARTICLE | February 21, 2017

Stimulated Raman spectroscopic imaging by microsecond delay-line tuning

Proc. SPIE. 10069, Multiphoton Microscopy in the Biomedical Sciences XVII

KEYWORDS: Mirrors, Optical amplifiers, Imaging systems, Spectroscopy, Imaging spectroscopy, Raman spectroscopy, Spectral resolution, Raman scattering, Picosecond phenomena, Bragg cells

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PROCEEDINGS ARTICLE | February 21, 2017

Sparsely-sampled hyperspectral stimulated Raman scattering microscopy: a theoretical investigation

Proc. SPIE. 10069, Multiphoton Microscopy in the Biomedical Sciences XVII

KEYWORDS: Hyperspectral imaging, Multiphoton microscopy, Biomedical optics, 3D image reconstruction, Data modeling, Image segmentation, Microscopy, Image restoration, Image acquisition, Data acquisition, Inverse problems, Image quality, Nonlinear optics, Raman scattering, 3D image processing

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PROCEEDINGS ARTICLE | April 27, 2016

High-speed intravascular photoacoustic imaging of lipid-laden plaque at 1.7 micron (Conference Presentation)

Proc. SPIE. 9708, Photons Plus Ultrasound: Imaging and Sensing 2016

KEYWORDS: Laser sources, Potassium, Optical parametric oscillators, Mechanics, Imaging systems, High power lasers, Image resolution, Photoacoustic imaging, Photoacoustic spectroscopy, Lead

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PROCEEDINGS ARTICLE | March 11, 2015

Assessing carotid atherosclerosis by fiber-optic multispectral photoacoustic tomography

Proc. SPIE. 9323, Photons Plus Ultrasound: Imaging and Sensing 2015

KEYWORDS: Breast, Fiber optics, Imaging systems, Blood, Ultrasonography, Arteries, Multispectral imaging, Photoacoustic tomography, Chemical analysis, Acquisition tracking and pointing

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SPIE Journal Paper | August 7, 2014

Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy

Chun-Rui Hu, Delong Zhang, Mikhail Slipchenko, Ji-Xin Cheng, Bing Hu

JBO Vol. 19 Issue 08

KEYWORDS: Axons, In vivo imaging, Microscopy, Nerve, Signal processing, Imaging systems, Image segmentation, Real time imaging, Raman scattering, Visualization

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SPIE Journal Paper | March 27, 2013

Compact high power barium nitrite crystal-based Raman laser at 1197 nm for photoacoustic imaging of fat

Rui Li, Mikhail Slipchenko, Pu Wang, Ji-Xin Cheng

JBO Vol. 18 Issue 04

KEYWORDS: Raman spectroscopy, Laser crystals, Crystals, Photoacoustic imaging, Barium, Neodymium, YAG lasers, Energy efficiency, Tissues, Optical parametric oscillators

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PROCEEDINGS ARTICLE | March 4, 2013

Vibration-based photoacoustic tomography

Proc. SPIE. 8581, Photons Plus Ultrasound: Imaging and Sensing 2013

KEYWORDS: Signal to noise ratio, Crystals, Nd:YAG lasers, Raman spectroscopy, Tomography, Photoacoustic tomography, Laser crystals, Signal detection, Pulsed laser operation, Laser bonding

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PROCEEDINGS ARTICLE | February 22, 2013

Spectroscopic SRS imaging with a time-lens source synchronized to a femtosecond pulse shaper

Proc. SPIE. 8588, Multiphoton Microscopy in the Biomedical Sciences XIII

KEYWORDS: Hyperspectral imaging, Microscopes, Femtosecond phenomena, Modulation, Spectroscopy, Imaging spectroscopy, Chromium, Raman spectroscopy, Chemical analysis, Picosecond phenomena

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SPIE Journal Paper | September 14, 2012

Mapping lipid and collagen by multispectral photoacoustic imaging of chemical bond vibration

Pu Wang, Ping Wang, Han-Wei Wang, Ji-Xin Cheng

JBO Vol. 17 Issue 9

KEYWORDS: Collagen, Tissues, Multispectral imaging, Photoacoustic spectroscopy, Photoacoustic imaging, Absorption, Chemical analysis, Photoacoustic microscopy, Near infrared, Optical parametric oscillators

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PROCEEDINGS ARTICLE | February 23, 2012

Hyperspectral vibrational photoacoustic imaging of lipids and collagen

Proc. SPIE. 8223, Photons Plus Ultrasound: Imaging and Sensing 2012

KEYWORDS: Hyperspectral imaging, Near infrared, Tissues, Image resolution, Collagen, Photoacoustic imaging, Chemical analysis, Photoacoustic spectroscopy, Neodymium, Absorption

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SPIE Journal Paper | October 1, 2011

Longitudinal <i>in vivo</i> coherent anti-Stokes Raman scattering imaging of demyelination and remyelination in injured spinal cord

Yunzhou Shi, Riyi Shi, Ji-Xin Cheng, Delong Zhang, Terry Huff, Xiaofei Wang, Xiao-Ming Xu

JBO Vol. 16 Issue 10

KEYWORDS: CARS tomography, Spinal cord, In vivo imaging, Tissues, Axons, Visualization, Surgery, Microscopes, Therapeutics, Injuries

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SPIE Journal Paper | October 1, 2011

Paranodal myelin retraction in relapsing experimental autoimmune encephalomyelitis visualized by coherent anti-Stokes Raman scattering microscopy

Yan Fu, Ji-Xin Cheng, Terra Frederick, Gwendolyn Goings, Stephen Miller, Terry Huff

JBO Vol. 16 Issue 10

KEYWORDS: Spinal cord, CARS tomography, Raman spectroscopy, Microscopy, Tissues, Proteins, Optical fibers, Visualization, Axons, Confocal microscopy

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PROCEEDINGS ARTICLE | March 1, 2011

A femtosecond stimulated Raman loss (fSRL) microscope for highly sensitive bond-selective imaging

Proc. SPIE. 7903, Multiphoton Microscopy in the Biomedical Sciences XI

KEYWORDS: Infrared imaging, Microscopes, Femtosecond phenomena, Signal attenuation, Microscopy, Luminescence, Raman spectroscopy, Raman scattering, Picosecond phenomena, 3D image processing

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SPIE Journal Paper | February 1, 2011

Stent-induced coronary artery stenosis characterized by multimodal nonlinear optical microscopy

Han-Wei Wang, Ji-Xin Cheng, Michael Sturek, Vlad Simianu, Matthew Locker

JBO Vol. 16 Issue 02

KEYWORDS: Collagen, Arteries, Nonlinear optics, Image segmentation, Microscopy, Optical microscopy, Quantitative analysis, Visualization, Tissues, Metals

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SPIE Journal Paper | February 1, 2011

Special Section Guest Editorial: Coherent Raman Imaging Techniques and Biomedical Applications

Eric Potma, Ji-Xin Cheng, X. Sunney Xie

JBO Vol. 16 Issue 02

KEYWORDS: Raman spectroscopy, Biomedical optics, Chromium, Coherence imaging, Tissues, Microscopes, Cameras, Photography, Biology, Microscopy

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PROCEEDINGS ARTICLE | February 24, 2010

Photothermal heterodyne phase imaging of gold seed and germanium nanowire

Proc. SPIE. 7564, Photons Plus Ultrasound: Imaging and Sensing 2010

KEYWORDS: Gold, Modulation, Nanoparticles, Germanium, Heterodyning, Phase imaging, Nanomaterials, Mass attenuation coefficient, Laser beam diagnostics, Nanowires

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PROCEEDINGS ARTICLE | February 13, 2009

Coupling CARS with multiphoton fluorescence and high harmonic generation imaging modalities using a femtosecond laser source

Proc. SPIE. 7183, Multiphoton Microscopy in the Biomedical Sciences IX

KEYWORDS: Microscopes, Second-harmonic generation, Optical parametric oscillators, Femtosecond phenomena, Liver, Tissues, Sensors, Picosecond phenomena, CARS tomography, Signal detection

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PROCEEDINGS ARTICLE | February 15, 2008

Imaging arterial cells, atherosclerosis, and restenosis by multimodal nonlinear optical microscopy

Proc. SPIE. 6860, Multiphoton Microscopy in the Biomedical Sciences VIII

KEYWORDS: Microscopes, Foam, Visualization, Tissues, Image segmentation, Microscopy, Arteries, Collagen, Nonlinear optics, CARS tomography

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SPIE Journal Paper | September 1, 2007

Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy

Thuc Le, Ingeborg Langohr, Matthew Locker, Michael Sturek, Ji-Xin Cheng

JBO Vol. 12 Issue 05

KEYWORDS: Collagen, Second-harmonic generation, Arteries, Microscopy, Nonlinear optics, Molecular imaging, CARS tomography, Signal detection, Microscopes, Optical microscopy

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PROCEEDINGS ARTICLE | February 10, 2007

Epi-detected Coherent anti-Stokes Raman Scattering Imaging of Deep Tissues in vivo

Proc. SPIE. 6442, Multiphoton Microscopy in the Biomedical Sciences VII

KEYWORDS: Polarization, Nerve, Tissues, Microscopy, Ear, In vivo imaging, Signal generators, CARS tomography, Signal detection, Axons

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PROCEEDINGS ARTICLE | February 5, 2007

Plasmon-resonant nanorods as multimodal agents for two-photon luminescence imaging and photothermal therapy

Proc. SPIE. 6448, Colloidal Quantum Dots for Biomedical Applications II

KEYWORDS: Gold, Near infrared, Plasmons, Tumors, Nanoparticles, Luminescence, In vivo imaging, Signal detection, Absorption, Nanorods

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PROCEEDINGS ARTICLE | June 17, 2002

Recent advances on Coherent Anti-Strokes Raman scattering (CARS) microscopy

Proc. SPIE. 4620, Multiphoton Microscopy in the Biomedical Sciences II

KEYWORDS: Confocal microscopy, Infrared imaging, Microscopes, Proteins, Microscopy, Luminescence, Diffusion, Raman spectroscopy, Cell death, CARS tomography

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