A new borate polymer PAA-ran-PAAPBA that can adsorb glucose specifically is introduced into the glucose
measurement based on surface plasmon resonance. Six and twelve layers of borate polymer are bound onto the SPR
sensors respectively through the layer-by-layer self-assembly binding method, and then the effect of different layers on
the glucose concentration measurement is studied. The experiment is conducted in three concentration ranges,
1~10mg/dL, 10~100mg/dL and 100~1000mg/dL. The Results show that the performance of 12-layer-polymer sensor is
better than that of the 6-layer-polymer sensor in the first two ranges, and the measuring result has no big difference in the
range of 100~1000mg/d. It indicates that the enhancement of polymer layer on the surface of SPR sensor can
dramatically improve the glucose measurement in the low concentration range.
Minimally-invasive human blood glucose detection can be realized by measuring the glucose concentration of interstitial fluid to predict the blood glucose level. As the amount of transdermally extracted interstitial fluid was minimal and its composition was complex, a glucose measurement method by surface plasmon resonance (SPR) based on PAA-ran-PAAPBA polymer binding was proposed. The polymer was immobilized on the gold film of SPR sensor using layer-by-layer self-assembly technique to capture the glucose molecules in interstitial fluid to realize the detection of glucose concentration with high precision. 2~1000mg/dL glucose solutions were measured utilizing the SPR sensor by polymer binding. The fitting degrees were 0.90177 and 0.99509 in the range of 2~10mg/dL and 25~1000mg/dL respectively. The dynamic dissociation process of glucose molecules from PAA-ran-PAAPBA was verified to be able to satisfy the requirements of the human blood glucose continuous monitoring in clinics.