Many imaging technologies, such as two photon microscopy (TPM), second generation microscopy (SHG) and photoacoustic microscopy (PAM), have successfully demonstrated the ability to extract anatomical, functional and molecular information of biological samples. However, they always fail to obtain comprehensive information from biological tissue due to single imaging modality. To address this limitation, we developed a multimodal microscopy with fully integrated PAM, TPM and SHG. The home-built multimodal microscopy system enable label-free imaging of biomedical tissue with sub-micron resolution in vivo and in vitro. The results may offer a new tool to provide the capable of fast comprehensive information capturing for biological studies.