KEYWORDS: Decision support systems, In vitro testing, Animal model studies, Life sciences, Inflammation, Colon, Absorbance, Statistical analysis, Sodium, Water
To explore the effect of Coptis chinensis-Scutellaria baicalensis on ulcerative colitis in mice and its antioxidant and anti-inflammatory effects in vitro. Sodium dextran sulfate (DSS) was used to induce acute ulcerative colitis in mice, lipopolysaccharide (LPS) was used to induce cell inflammation model, and ABTS and DPPH models were used to evaluate antioxidant capacity. The results showed that the drug had no significant effect on colitis, but cell experiments in vitro showed that the drug had significant anti-inflammatory effect and could scavenge ABTS and DPPH free radicals. Coptis chinensis-scutellaria baicalensis has anti-inflammatory and anti-oxidative effects.
As the traditional Chinese medicine, artemisiae argyi folium has various functions such as analgesia, antitumor, and anti-hypertension. To study antioxidant activity of artemisiae argyi folium (AAF) in vitro. The antioxidant activity of AAF was evaluated by ABTS assay, DPPH experiment, OH test and lipid peroxidation. The hydrogen peroxide-induced RAW264.7 cell oxidative damage model was established to assess its antioxidant activity. The results showed that the AAF had strong antioxidant activity and the IC50 on ABTS radicals, DPPH radicals and hydroxyl radicals were 80.88, 813.63, 2458.67 and 94.86μg/ml. MTT experiments showed that AAF was not cytotoxic. The quantitative polymerase chain reaction demonstrate the antioxidant activity of AAF. AAF has great potential for development.
Mulberry, as medicinal and edible traditional Chinese herb, has many physiological functions. The present study aims to evaluating the antioxidant activity in vitro and immune modulation of mulberry extracts (ME). The antioxidant ability of ME was comprehensively evaluated by different systems, including the scavenging rates of DPPH and ABTS radical. The mice spleen lymphocyte transformation experiment and the mice peritoneal macrophage phagocytosis chicken red blood cells test were used to observe the effects of ME on specific cellular immunity and nonspecific immunity. The results showed that the IC50 of ME on DPPH and ABTS radical were 521.10 and 1049.44 μg/mL, respectively. In addition, ME can promote the proliferation of spleen lymphocytes and strengthen the phagocytic function of monocyte macrophages. Mulberry has antioxidant benefit and immune boosting effect, which provide a reference for more adequate development and utilization of mulberry resources.
As the traditional Chinese medicine, artemisiae argyi folium has various functions such as analgesia, antitumor, and anti-hypertension. To study antioxidant activity in vitro and cell-based anti-inflammatory activity of artemisiae argyi folium polysaccharide (AAFP). The antioxidant activity of AAFP was investigated by ABTS+ • clearance rate, DPPH•clearance rate, • OH clearance rate and FRAP assays. The lipopolysaccharide-induced RAW264.7 cell inflammation model was established to assess its anti-inflammatory activity. The results showed that the AAFP had strong antioxidant activity and the IC50 on ABTS radicals, DPPH radicals, hydroxyl radicals, and the ferric reducing antioxidant power were 74.41, 268.5, 350.2μg/ml and the FRAP value was 163.5μg/ml. MTT experiments showed IC50 of AAFP was 441.5μg/ml. The quantitative polymerase chain reaction demonstrate the pro-inflammatory activity of AAFP. Immunological assay showed that AAFP significantly promoted lymphocyte proliferation. AAFP have huge potential to be developed as functional foods and medicines.
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