In this paper, HMME-TiO2 nanocomposites was synthesized and characterized through TEM， Uv-vis spectra, Zeta potential, FTIR spectra. The characterization results show that HMME was successfully conjugated onto the surface of TiO2. It can be seen from the TEM images the average size of HMME-TiO2 conjugation is nearly spherical and the particle size range from 20 to 28 nm. Compared with HMME, the B bands of HMME-TiO2 were much broader and lower while in the region of Q bands the absorption peaks of HMME-TiO2 are higher than that of HMME. Encapsulation efficiency of HMME-loaded TiO2 was assessed and calculated as 45.46%. FTIR spectra show the bonding between TiO2 and HMME was through the hydrogen-bonding between COOH and OH bonds. Fluorescence microscope results demonstrated HMME-TiO2 mainly distributed in the membrane and cytoplasm of SCC cells and its best incubation time is six hours. After treated with HMME-TiO2 plus light irradiation (1.8J/cm2 , 632nm), the viability of SCC cells turned to 32.96% is much lower than that treated with HMME plus light irradiation. It can be concluded that the combination of HMME and TiO2 will enhance the PDT efficiency of HMME. In the process of HMME-TiO2 mediated PDT, as a kind of photosensitizer HMME can induce the death of SCC cells, meanwhile it can transform electron to the conductive band of TiO2 stimulating the photocatalytic activity of TiO2 under visible light. The photocatalytic of TiO2 can also induce the death of SCC cells. The combination of these two effects lead to more SCC cells died.
A LED array is used as PDT light source in a photo-reaction chamber. The LED array is alterable in wavelength by
replacing different LEDs, stable in output power, adjustable in power value and spot size; the geometry of the LED array
is flat shape. Based on the experiment of ALA-PDT, we measured and analyzed the spectrum characteristics and output
power of some different purple LEDs, selected one as PDT light source whose emission peak is near the absorption peak
of the ALA. The experiment on HL60 tumor cells in vitro demonstrates our photo-reaction chamber has visible
photodynamic effect on ALA-PDT.
Based on the morphological differences between normal, apoptosis and necrosis cell, a new method for detection cell viability is presented in the paper. The Jurkat cells samples were used for studying the relationship between morphological parameters and cell viability in the paper. According to the scatter charts of Jurkat cell roundness, radius ratio and area, the cell areas were mainly distributed from 40 μm2 to 80 μm2. The percentage of main areas in the two cells samples were analyzed statistically with values of 62.37% and 75.45 % respectively. Due to the mostly normal cell used by the experiment in the exponent growth period, a conclusion that the areas of normal cells were mainly distributed from 40 μm2 to 80 μm2 is presented. And the areas of apoptosis and necrosis cells are distributed in other range, i.e. less than 40 μm2 or more than 80 μm2. There are not any chemical medicaments needed to add in the samples by the method based on the videopicture in order to detect the states of cell samples. What it reflected by analyzing morphological parameters of cell are the true states of cell.
The characteristics of ALA absorption spectrum were studied by comparing the difference of ALA absorption spectrum in different conditions in the paper. The results of test show that the wavelength of the strongest absorption spectrum of ALA is 270nm. The relationship between the serum solution density and the absorption peak value is the inverse ratio. The obstructive effect will be more obvious when the density of serum is thicker. The best serum solution density is 10%. The amount of PpIX will be the biggest when the incubate time is suitable and the right time is 8 hours for Jurkat cell.
The characteristics of ALA emission spectrum and excitation spectrum were studied by comparing the difference of ALA emission spectrum and excitation spectrum in different incubation conditions of Jurkat cell in the paper. The results of test show that the wavelength of the strongest emission spectrum of ALA is 375nm. The wavelength of the strongest excitation spectrum of ALA is 255nm and 290nm. The wavelength of 290nm is better to excite the ALA. Those results will be helpful to diagnose and detect the cancer.
A simple method was found to solve the shortage in the cell size measurement of flow cytometry (FCM). The real-time result can be give out by measure the laser intensity absorbed by the dyed cells. Cooperation with FCM, the distribution of cell size can be got.
This article describes the work of ALA-PDT to the HL60 cells, which includes parameters of ALA density, Culture time, light dose and optimal wavelength of irradiation, and summarizes the optimize project of experiment using ALA-PD to the HL60 leukaemic cells.
The UV fluorescence method for real-time monitoring concentration of sulfur dioxide is advanced method in the world at present. Some technical details of its sensor are interesting problems for instrument specialist and user. This paper introduces some design problems of Opto-electronic system with center wave 213.8nm of excitation light source and picking up signal over a range of wavelength 250-400nm. There problems include of spectrum optimal matching, elements characteristic, optimization of sensor system and analyze of experiment result. This research outcome will use to monitoring sulfur dioxide of smoke emitted from power plant.