Ever since the human genome was first sequenced, scientists have been inspired by possibilities of using genomic information for medical research. In recent year, new generation sequencing platform to deliver complete genome sequence data with higher throughputs are to be built to conduct genomic studies on a large scale. This requires the development of a wide field multi-channel fluorescence imager system. The complexity of this optical system for human genome sequencing application would also have specific optical coating challenges. For the objective lens system, it requires selection of multiple glass types with normal and anomalous dispersions in order to successfully correct chromatic aberrations to diffraction-limited level over a broad wavelength spectrum. The challenge is anti-reflection (AR) coatings need to be coated over these multi-glass types with various refractive index from 1.43 to 1.8 and operate through an extended range of broad spectrum range. In addition, auto-fluorescence of optical components and coatings applied to the lenses are considered an isotropic generation of the secondary stray light inside the system. This is undesirable and should be minimized. This research work presents the AR coating design strategy to accommodate the multiple glass types in the lens system over a broadband application range and the investigation results of achieving low auto-fluorescence through material selection and coating process control.