Prof. Jonathan C. Claussen Profile

Prof. Jonathan C. Claussen

Associate Professor at Iowa State Univ of Science and Technology

SPIE Involvement:

Conference Program Committee | Author

Proceedings Article | 16 August 2019 Presentation

Biomimetic nanosensors for measuring pathogenic bacteria in complex food matrices (Conference Presentation)

Daniela Oliveira, Loreen Stromberg, Cicero Pola, Kshama Parate, Nicholas Cavallaro, Jonathan Claussen, Eric McLamore, Carmen Gomes

Proceedings Volume 11020, 110200J (2019) https://doi.org/10.1117/12.2519523

KEYWORDS: Pathogens, Biomimetics, Matrices, Nanosensors, Polymers, Biosensors, Sensors, Graphene, Raman spectroscopy, Bacteria

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Listeria monocytogenes and Salmonella spp. are among the most common cause of foodborne illnesses that negatively affect consumers’ health and food producers’ finances and credibility. Techniques used to detect pathogens (e.g., total viable counts, polymerase chain reaction, and enzyme-linked immunosorbent assays) are time consuming and costly as they require laboratory conditions with trained personnel. To meet this demand without compromising public health concerns, highly sensitive and rapid sensors are needed in food processing facilities for pathogen detection to reduce cost and holding time for food products. Ideally, these sensors should be small, label-free, low cost, portable, and highly sensitive/selective. This study describes some recent approaches for creating biomimetic sensors by optimizing the bacteria capture efficiency without the need for pre-concentration and pre-labeling steps. Two in-field biosensors were developed for measuring pathogenic bacteria in food matrices. The first example consists of pH-responsive polymer nanobrushes embedded with platinum nanoparticles platform with enhanced limit of detection and sensitivity for quantification of Listeria monocytogenes in fresh vegetables. A new approach using a one-step metal and polymer simultaneous deposition was tested using two pH-sensitive polymers and a thiol-terminated DNA aptamer selective to surface protein internalin A of Listeria monocytogenes. The second example demonstrates development of pathogenic biosensors for chicken broth using antibodies and DNA aptamers selective to Salmonella Typhimurium adsorbed to aerosolized graphene interdigitated electrodes (IDEs). Devices were printed in polyimide tape and aerosolized graphene was thermally annealed. The integrity of the substrate was analyzed and the nano-biosensors were characterized for topography, pH-actuation, graphene content, and electroactivity using electron microscopy, cyclic voltammetry, and multiple spectroscopy techniques (Raman, Fourier-transform infrared, and electrochemical impedance). Electrochemical impedance spectroscopy was used to evaluate the signal and determine the limit of detection by evaluating the change in charge transfer resistance. The nano-biosensors have a detection limit of approximately 5 CFU.mL-1, and a response time of approximately 17 minutes (15 minutes incubation period). The pH-sensitive nanobrushes and graphene-based biosensors have a selectivity for the target pathogen of approximately 95% in vegetable and chicken broth, respectively. The designed biosensor platform showed great potential to replace current standard methods used by the food industry for rapid foodborne pathogenic bacteria detection.

Proceedings Article | 13 May 2016 Paper

A paper based graphene-nanocauliflower hybrid composite for point of care biosensing

S. Burrs, R. Sidhu, M. Bhargava, J. Kiernan-Lewis, N. Schwalb, Y. Rong, C. Gomes, J. Claussen, D. Vanegas, E. McLamore

Proceedings Volume 9863, 98630N (2016) https://doi.org/10.1117/12.2223346

KEYWORDS: Biosensing, Graphene, Point-of-care devices, Platinum, Composites, Glucose, Raman spectroscopy, Oxides, Biosensors, Sensors

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Proceedings Article | 13 May 2016 Paper

Effect of platinum nanoparticle deposition parameters on hydrogen peroxide transduction for applications in wearable electrochemical glucose biosensors

Allison Cargill, Kathrine Neil, John Hondred, Eric McLamore, Jonathan Claussen

Proceedings Volume 9863, 98630E (2016) https://doi.org/10.1117/12.2227204

KEYWORDS: Platinum, Glucose, Electrodes, Biosensors, Sensors, Nanoparticles, Hydrogen, Carbon, Blood, Metals

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Proceedings Article | 13 May 2016 Paper

Impedance biosensor for the rapid detection of Listeria spp. based on aptamer functionalized Pt-interdigitated microelectrodes array

R. Sidhu, Y. Rong, D. Vanegas, J. Claussen, E. McLamore, C. Gomes

Proceedings Volume 9863, 98630F (2016) https://doi.org/10.1117/12.2223443

KEYWORDS: Dielectric spectroscopy, Biosensors, Bacteria, Electrodes, Pathogens, Proteins, Chemical elements, Safety, Resistance, Platinum

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Proceedings Article | 13 May 2016 Paper

Bio-inspired patterned networks (BIPS) for development of wearable/disposable biosensors

E. McLamore, M. Convertino, John Hondred, Suprem Das, J. Claussen, D. Vanegas, C. Gomes

Proceedings Volume 9863, 986309 (2016) https://doi.org/10.1117/12.2223345

KEYWORDS: Biosensors, Biomimetics, Fractal analysis, Electrodes, Graphene, Sensor performance, Optical lithography, Glucose, Biosensing, Electrons

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Proceedings Article | 21 August 2015 Presentation + Paper

Modified kinetics of enzymes interacting with nanoparticles

Sebastián Díaz, Joyce Breger, Anthony Malanoski, Jonathan Claussen, Scott Walper, Mario Ancona, Carl Brown, Michael Stewart, Eunkeu Oh, Kimihiro Susumu, Igor Medintz

Proceedings Volume 9550, 95500K (2015) https://doi.org/10.1117/12.2188212

KEYWORDS: Sensors, Fluorescence resonance energy transfer, Neptunium, Luminescence, Nanoparticles, Molecules, Catalysis, Calibration, Molecular energy transfer, Absorbance

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Proceedings Article | 22 May 2014 Paper

Monitoring enzyme kinetic behavior of enzyme-quantum dot bioconjugates

Jonathan Claussen, Scott Walper, Kimihiro Susumu, Mario Ancona, Igor Medintz

Proceedings Volume 9107, 910710 (2014) https://doi.org/10.1117/12.2050791

KEYWORDS: Quantum dots, Nanocrystals, Metals, Semiconductors, Resistance, Quantum efficiency, Nanoparticles, In vivo imaging, Biosensing, Molecular photonics

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Proceedings Article | 1 October 2013 Paper

Enhancing molecular logic through modulation of temporal and spatial constraints with quantum dot-based systems that use fluorescent (Förster) resonance energy transfer

Jonathan Claussen, W. Russ Algar, Niko Hildebrandt, Kimihiro Susumu, Mario Ancona, Igor Medintz

Proceedings Volume 8817, 88170O (2013) https://doi.org/10.1117/12.2024287

KEYWORDS: Terbium, Logic, Fluorescence resonance energy transfer, Ultraviolet radiation, Resonance energy transfer, Modulation, Quantum dots, Luminescence, Resonance enhancement, Molecular energy transfer

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Showing 5 of 8 publications

Conference Committee Involvement (10)

Smart Biomedical and Physiological Sensor Technology XXI

22 April 2024 | National Harbor, Maryland, United States

Smart Biomedical and Physiological Sensor Technology XX

1 May 2023 | Orlando, Florida, United States

Smart Biomedical and Physiological Sensor Technology XIX

4 April 2022 | Orlando, Florida, United States

Smart Biomedical and Physiological Sensor Technology XVIII

12 April 2021 | Online Only, Florida, United States

Smart Biomedical and Physiological Sensor Technology XVI

15 April 2019 | Baltimore, MD, United States

Showing 5 of 10 Conference Committees

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