This study investigates the Candida albicans biofilm formation on different phases of titanium dioxide (TiO2) and titanium disulfide (TiS2) directly grown on titanium substrates. The Candida albicans growth is evaluated by counting the cells in media and surface as well as using colorimetric XTT assays for evaluating biofilm formation. Also, the viability and adhesion of human gingival fibroblast cells to the material's surface is investigated to show the non-toxicity and cell adhesion to these surface. TiS2 nanoflakes growth from titanium substrates showed significant improvement in both the cell attachments and antimicrobial effect.
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