Prof. Jörg Enderlein Profile

Prof. Jörg Enderlein

Professor/Biophysical Chemistry at Georg-August-Univ Göttingen

SPIE Involvement:

Conference Program Committee | Author | Editor | Instructor

Publications (45)

Proceedings Article | 13 March 2024 Presentation

Doubling the resolution of single-molecule localization microscopy with image scanning microscopy

Niels Radmacher, Oleksii Nevskyi, José Ignacio Gallea, Jan Christoph Thiele, Ingo Gregor, Jörg Enderlein

Proceedings Volume PC12849, PC128490I (2024) https://doi.org/10.1117/12.3004932

KEYWORDS: Image resolution, Microscopes, Fluorescence, Multiplexing, Detector arrays, Confocal microscopy, Super resolution microscopy, Software development, Single photon detectors, Open source software

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Proceedings Article | 15 March 2023 Presentation

Accurate photophysical transition rates of fluorophores (Conference Presentation)

Ingo Gregor, Damir Sakhapov, Jörg Enderlein

Proceedings Volume PC12386, PC123860B (2023) https://doi.org/10.1117/12.2655114

KEYWORDS: Luminescence, Fluorescence correlation spectroscopy, Molecules, Confocal microscopy, Time metrology, Temporal resolution, Signal to noise ratio, Rhodamine, Photolysis, Molecular electronics

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Proceedings Article | 2 March 2022 Poster

Fluorescence lifetime image scanning microscopy

Ingo Gregor, Niels Rademacher, Max Tillmann, Matthias Patting, Jörg Enderlein, Felix Koberling

Proceedings Volume PC11967, PC119670J (2022) https://doi.org/10.1117/12.2625458

KEYWORDS: Microscopy, Luminescence, Single photon detectors, Microscopes, Fluorescence lifetime imaging, Confocal microscopy, Tissues, Spatial resolution, Single photon, Sensors

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Proceedings Article | 2 March 2022 Presentation

Studying membrane biophysics using Graphene-induced energy-transfer

Ingo Gregor, Arindam Ghosh, Tao Chen, Sufi Raja, Alexey Chizhik, Christoph Schmidt, Jörg Enderlein

Proceedings Volume PC11967, PC1196707 (2022) https://doi.org/10.1117/12.2616863

KEYWORDS: Energy transfer, Biophysics, Molecules, Fluorescence lifetime imaging, Acquisition tracking and pointing, Photonics, Near field optics, Near field, Molecular energy transfer, Microscopy

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Proceedings Article | 7 April 2020 Presentation

Fluorescence speckle image correlation spectroscopy (Conference Presentation)

Anirban Sarkar, Irène Wang, Aditya Katti, Jörg Enderlein, Jacques Derouard, Antoine Delon

Proceedings Volume 11351, 1135108 (2020) https://doi.org/10.1117/12.2558129

KEYWORDS: Speckle, Scattering, Fluorescence correlation spectroscopy, Spectroscopy, Light scattering, Luminescence, Fluorescence spectroscopy, Imaging spectroscopy, Particles, Speckle pattern

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Fluorescence correlation spectroscopy is used extensively for quantitative characterization of biomolecules at very low concentration. However, light aberration and scattering from the tissues are two major factors that affect the results strongly. Although adaptive optics arrangement can correct the aberrations of light to some extent, it fails completely to eliminate the light scattering effect. Recently, exploiting the fact that autocorrelation of a speckle pattern is a sharply peaked point spread function and the optical memory effect, non-invasive imaging of fluorescent sample through a scattering medium has been possible. However, it is also very challenging to measure the dynamic properties of the fluorescent molecules or particles through a scattering layer due to poor signal to noise ratio. In this study, we employ a modality based on speckle cross-correlation enabled via optical memory effect to study two dimensional (2D) diffusion of fluorescent particles hidden behind a scattering film. We realized a 2D diffusing model system by confining fluorescent polystyrene beads of 1µm diameter at the water/air interface behind a TiO2 diffuser. The experimental set up was built up in an epifluorescence configuration. The fluorescent beads were excited by an illumination speckle generated by the incident light in a plane-wave geometry while passing through the disordered TiO2 film. Similarly, the emitted fluorescent signal also traversed through the same TiO2 film to generate the detection speckle, which was eventually recorded by a high frame rate CMOS camera. The experimental set up has also been modelled numerically, where speckle pattern has been generated by a spherical wave, transmitted through a scattering object in an optical microscope. Moreover, the dependence of the speckle size on the numerical aperture, magnification, and the distance of the focal plane from the bead plane has also been studied. The numerical results have been compared with the experimental values to estimate the speckle size. Furthermore, we have evaluated the 2D diffusion constant by monitoring the widening of the 2D speckle cross-correlation function versus lag time. This result has been compared with that obtained with the single particle tracking method without the scattering layer. Quantitative agreement between the results obtained by the speckle cross-correlations and the single particle tracking technique without the diffuser establishes the potential application of this technique in correlation spectroscopy. Superimposed multiple beads speckle patterns were also studied and the results will be presented in the conference.

Proceedings Article | 9 March 2020 Presentation

Fluorescence lifetime image scanning microscopy (Conference Presentation)

Ingo Gregor, Niels Radmacher, Jörg Enderlein

Proceedings Volume 11246, 1124606 (2020) https://doi.org/10.1117/12.2546532

KEYWORDS: Microscopy, Fluorescence lifetime imaging, Sensors, Luminescence, Confocal microscopy, Microscopes, Cameras, Electronics, Signal detection, Super resolution

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Proceedings Article | 9 March 2020 Presentation

Rapid non-linear image scanning microscopy (Conference Presentation)

Ingo Gregor, Jörg Enderlein, Robert Ros

Proceedings Volume 11244, 112440D (2020) https://doi.org/10.1117/12.2541142

KEYWORDS: Microscopy, Multiphoton microscopy, Super resolution, Second-harmonic generation, Microscopes, Fluorescence lifetime imaging, Confocal microscopy, Collagen, Image acquisition, Organisms

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Proceedings Article | 4 March 2019 Presentation

Graphene-induced energy transfer: resolving distances at the Angström scale (Conference Presentation)

Ingo Gregor, Arindam Ghosh, Akshita Sharma, Sebastian Isbaner, Alexey Chizhik, Narain Karedla, Jörg Enderlein

Proceedings Volume 10884, 108840H (2019) https://doi.org/10.1117/12.2507939

KEYWORDS: Energy transfer, Molecules, Graphene, Super resolution microscopy, Super resolution, Plasmons, Metals, Thin films, Resonance energy transfer, Fluorescence resonance energy transfer

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In recent years single-molecule localization super-resolution microscopy (SMLM) has become an indispensable tool for many fields of research. Here, for any image of a single molecule one determines its center position with much higher accuracy than the size of that image itself. A challenge of SMLM is to achieve super-resolution also along the third dimension. Recently, Metal-Induced Energy Transfer or MIET [1,2] was introduced. It exploits the energy transfer from an excited fluorophore to plasmons in a thin metal film. Similar to Förster Resonance Energy Transfer (FRET), this coupling shows a strong distance dependence, but over a range up to 150 nm and enables axial localization of fluorophores with 5-6 nm resolution at a photon budget of 1000 photons. [3,4] Here, we show that using a graphene layer the localization accuracy of MIET reaches Ångström accuracy. At such accuracy, minute details such as nanometer-scale roughness of the sample surfaces becomes important. For proof of principle, we determined absolute distances of single molecules from a surface for samples with an a priori well-known sample geometry. We spin-coated fluorescent dye molecules (Atto655) on top of three different substrates with spacer thickness values of 10, 15, and 20 nm, defining the distance of the molecules from the graphene layer. Next, we determined the thickness of supported lipid bilayers (SLBs) by localizing fluorescent dyes attached to lipid head groups in the bottom and top leaflet of the SLB. We have demonstrated that by using graphene as the energy acceptor in MIET, the axial localization accuracy and resolution reaches sub-nanometer levels at photon budgets which are typical in conventional SMLM experiments. An interesting feature of graphene-MIET is that it provides an axial localization accuracy which now surpasses significantly that of lateral localization provided by most SMLM approaches. [1] A. I. Chizhik et al. Nat. Photonics 8, 124 (2014). [2] S. Isbaner et al. Nano Lett. 18, 2616 (2018). [3] N. Karedla et al. ChemPhysChem 15, 705 7 (2014). [4] N. Karedla et al. J. Chem. Phys. 148, 204201 (2018).

Proceedings Article | 24 April 2017 Presentation

Superresolution upgrade for confocal spinning disk systems using image scanning microscopy (Conference Presentation)

Sebastian Isbaner, Dirk Hähnel, Ingo Gregor, Jörg Enderlein

Proceedings Volume 10071, 100710F (2017) https://doi.org/10.1117/12.2255813

KEYWORDS: Confocal microscopy, Microscopy, Super resolution, Stereoscopy, Imaging systems, 3D image processing, Image resolution, 3D acquisition, Diffraction, Live cell imaging

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Proceedings Article | 24 April 2017 Presentation

metal-induced energy transfer (MIET) (Conference Presentation)

Narain V. Karedla, Sebastian Isbaner, Alexey Chizhik, Ingo Gregor, Joerg Enderlein, Anna Chizhik

Proceedings Volume 10071, 1007106 (2017) https://doi.org/10.1117/12.2255744

KEYWORDS: Energy transfer, Molecules, Fluorescence resonance energy transfer, Metals, Luminescence, Surface plasmons, Distance measurement, Molecular energy transfer, Resonance energy transfer, Dielectrics

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Proceedings Article | 24 April 2017 Presentation

Non-linear image scanning microscopy (Conference Presentation)

Ingo Gregor, Robert Ros, Jörg Enderlein

Proceedings Volume 10071, 100710C (2017) https://doi.org/10.1117/12.2255891

KEYWORDS: Multiphoton microscopy, Microscopy, Second-harmonic generation, Super resolution, Luminescence, Collagen, Image acquisition, Microscopes, Organisms, Image quality

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Proceedings Article | 27 April 2016 Presentation

Fourier-interpolation superresolution optical fluctuation imaging (fSOFi) (Conference Presentation)

Joerg Enderlein, Simon Stein, Anja Huss, Dirk Hähnel, Ingo Gregor

Proceedings Volume 9714, 97140K (2016) https://doi.org/10.1117/12.2212289

KEYWORDS: Super resolution, Optical imaging, Stochastic processes, Microscopy, Image enhancement, Luminescence, Resolution enhancement technologies, Spatial resolution, Image analysis, Molecules

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Proceedings Article | 27 April 2016 Presentation

Study conformational dynamics of intrinsically disordered protein by PET-FCS (Conference Presentation)

Joerg Enderlein, Man Zhou, Qui Van, Ingo Gregor

Proceedings Volume 9714, 971404 (2016) https://doi.org/10.1117/12.2212247

KEYWORDS: Proteins, Fluorescence correlation spectroscopy, Positron emission tomography, Imaging spectroscopy, Single molecule spectroscopy, Super resolution, Current controlled current source

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Proceedings Article | 27 April 2016 Presentation

Dead-time correction for high-throughput fluorescence lifetime imaging microscopy (Conference Presentation)

Joerg Enderlein, Daja Ruhlandt, Anna Chithik, René Ebrecht, Fred Wouters, Ingo Gregor

Proceedings Volume 9714, 971407 (2016) https://doi.org/10.1117/12.2212167

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Pulsed laser operation, Microscopy, Single photon, Molecules, Molecular lasers, Time metrology, Photodetectors, Photon counting

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Proceedings Article | 4 March 2014 Paper

Single-molecule fluorescence inside solid-state nanochannels

Siddharth Ghosh, Manoj Kumbhakar, Mitja Platen, Ingo Gregor, Jörg Enderlein

Proceedings Volume 8950, 895008 (2014) https://doi.org/10.1117/12.2035236

KEYWORDS: Molecules, Silica, Solid state physics, Silicon, Solid state lasers, Nanolithography, Confocal microscopy, Solid state electronics, Finite element methods, Atomic force microscopy

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Proceedings Article | 22 February 2013 Paper

SOFI of GABAB neurotransmitter receptors in hippocampal neurons elucidates intracellular receptor trafficking and assembly

Anja Huss, Omar Ramírez, Felipe Santibáñez, Andrés Couve, Steffen Härtel, Jörg Enderlein

Proceedings Volume 8590, 85900N (2013) https://doi.org/10.1117/12.2006215

KEYWORDS: Receptors, Neurons, Plasma, Luminescence, Microscopy, Super resolution, Neurotransmitters, Optical imaging, Proteins, Image enhancement

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Proceedings Article | 11 February 2011 Paper

Fast algorithms for the analysis of spectral FLIM data

I. Gregor, B. Krämer, F. Koberling, R. Erdmann, J. Enderlein, M. Wahl, S. Fore

Proceedings Volume 7903, 790330 (2011) https://doi.org/10.1117/12.873386

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Fluorescence resonance energy transfer, Signal detection, Data conversion, Single photon, Picosecond phenomena, Microscopes, Statistical analysis, Fluorescence spectroscopy

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Proceedings Article | 25 February 2009 Paper

Optical microresonator modifies the efficiency of the fluorescence resonance energy transfer in the autofluorescent protein DsRed

F. Schleifenbaum, K. Elgass, M. Steiner, J. Enderlein, S. Peter, A. Meixner

Proceedings Volume 7185, 718504 (2009) https://doi.org/10.1117/12.809325

KEYWORDS: Luminescence, Chromophores, Mirrors, Microresonators, Proteins, Fluorescence resonance energy transfer, Energy transfer, Free space, Energy efficiency, Near field optics

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SPIE Journal Paper | 1 September 2008 Open Access

Experimental and theoretical evaluation of surface plasmon-coupled emission for sensitive fluorescence detection

Michal Trnavsky, Jörg Enderlein, Thomas Ruckstuhl, Colette McDonagh, Brian MacCraith

JBO, Vol. 13, Issue 05, 054021, (September 2008) https://doi.org/10.1117/12.10.1117/1.2978067

KEYWORDS: Luminescence, Metals, Molecules, Silica, Data modeling, Surface plasmons, Silver, Glasses, Fluorescence spectroscopy, Refractive index

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Proceedings Article | 21 February 2008 Paper

Latest applications for 2-focus fluorescence correlation spectroscopy

T. Dertinger, I. von der Hocht, A. Loman, R. Erdmann, J. Enderlein

Proceedings Volume 6862, 686202 (2008) https://doi.org/10.1117/12.762365

KEYWORDS: Fluorescence correlation spectroscopy, Diffusion, Refractive index, Calcium, Molecules, Luminescence, Prisms, Glasses, Proteins, Pulsed laser operation

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Proceedings Article | 11 May 2007 Paper

Fluorescence lifetime correlation spectroscopy

Jana Humpolickova, Jan Sýkora, Petr Kapusta, Michael Wahl, Ales Benda, Jorg Enderlein, Martin Hof

Proceedings Volume 6583, 658308 (2007) https://doi.org/10.1117/12.736637

KEYWORDS: Fluorescence correlation spectroscopy, Luminescence, Diffusion, Fluorescence spectroscopy, Sensors, Spectroscopy, Single photon, Molecules, Particles, Microscopes

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Proceedings Article | 14 February 2007 Paper

Monitoring of small conformational changes by high-precision measurements of hydrodynamic radius with 2-focus fluorescence correlation spectroscopy (2fFCS)

Thomas Dertinger, Iris von der Hocht, Ingo Gregor, Konstantin Komolov, Karl-Wilhelm Koch, Jörg Enderlein

Proceedings Volume 6444, 64440H (2007) https://doi.org/10.1117/12.704930

KEYWORDS: Calcium, Fluorescence correlation spectroscopy, Proteins, Luminescence, Pulsed laser operation, Prisms, Beam splitters, Polarization, Molecules, Diffusion

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Proceedings Article | 27 February 2006 Paper

Dynamic saturation optical microscopy

Jan Sýkora, Thomas Dertinger, Jörg Enderlein

Proceedings Volume 6092, 60920E (2006) https://doi.org/10.1117/12.647987

KEYWORDS: Luminescence, Microscopy, Diffraction, Modulation, Optical microscopy, Spatial resolution, Microscopes, Molecules, Sensors, Point spread functions

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Proceedings Article | 27 February 2006 Paper

Measuring precise diffusion coefficients with two-focus fluorescence correlation spectroscopy

Thomas Dertinger, Ingo Gregor, Iris von der Hocht, Rainer Erdmann, Benedikt Krämer, Felix Koberling, Rudolf Hartmann, Jörg Enderlein

Proceedings Volume 6092, 609203 (2006) https://doi.org/10.1117/12.651053

KEYWORDS: Diffusion, Fluorescence correlation spectroscopy, Luminescence, Molecules, Pulsed laser operation, Polarization, Beam splitters, Objectives, Photodetectors, Data modeling

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Proceedings Article | 29 March 2005 Paper

Advanced multifocus confocal laser scanning microscope for single molecule studies

Thomas Dertinger, Felix Koberling, Ales Benda, Rainer Erdmann, Martin Hof, Joerg Enderlein

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.611147

KEYWORDS: Molecules, Luminescence, Confocal microscopy, Diffusion, Fluorescence correlation spectroscopy, Pulsed laser operation, Particles, Microscopes, Fluorescence spectroscopy, Photodetectors

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Proceedings Article | 29 March 2005 Paper

Art and artifacts of fluorescence correlation spectroscopy

Joerg Enderlein, Ingo Gregor, Digambara Patra, Thomas Dertinger

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.588527

KEYWORDS: Diffusion, Fluorescence correlation spectroscopy, Molecules, Refractive index, Confocal microscopy, Wavefronts, Monochromatic aberrations, Objectives, Sensors, Molecular interactions

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Proceedings Article | 19 June 2003 Paper

Multiparameter single-molecule fluorescence measurements of DNA intercalating fluorophores

Benjamin Bowen, Jorg Enderlein, Neal Woodbury

Proceedings Volume 4962, (2003) https://doi.org/10.1117/12.486348

KEYWORDS: Luminescence, Fluorescence spectroscopy, Photons, Molecules, Data modeling, Spectroscopy, Mirrors, Optical filters, Fluorescence correlation spectroscopy, Diffusion

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Proceedings Article | 19 June 2003 Paper

Ab initio modeling of fluorescence fluctuation spectroscopy

Joerg Enderlein, Martin Bohmer

Proceedings Volume 4962, (2003) https://doi.org/10.1117/12.478906

KEYWORDS: Fluorescence correlation spectroscopy, Diffusion, Luminescence, Molecules, Data modeling, Fluorescence spectroscopy, Spectroscopy, Convolution, Objectives, 3D modeling

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Proceedings Article | 28 March 2002 Paper

Radiative decay engineering: new vistas for fluorescent materials with exceptional properties

Joerg Enderlein

Proceedings Volume 4634, (2002) https://doi.org/10.1117/12.463831

KEYWORDS: Luminescence, Metals, Molecules, Electromagnetism, Fluorescence spectroscopy, Coating, Fluorescent materials, Spherical lenses, Dielectrics, Absorption

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Proceedings Article | 28 March 2002 Paper

Fluorescence lifetime imaging system with nm-resolution and single-molecule sensitivity

Michael Wahl, Hans-Juergen Rahn, Uwe Ortmann, Rainer Erdmann, Martin Boehmer, Joerg Enderlein

Proceedings Volume 4634, (2002) https://doi.org/10.1117/12.463829

KEYWORDS: Molecules, Luminescence, Picosecond phenomena, Scanners, Semiconductor lasers, Fluorescence lifetime imaging, Single photon, Clocks, Sensors, Electronics

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Proceedings Article | 21 April 2000 Paper

Quantitative fluorescence spectroscopy of single molecules on surfaces

Martin Boehmer, Joerg Enderlein, Stefan Seeger

Proceedings Volume 3922, (2000) https://doi.org/10.1117/12.383339

KEYWORDS: Molecules, Luminescence, Fluorescence spectroscopy, Molecular lasers, Molecular spectroscopy, Photons, Confocal microscopy, Absorption, Microscopes, Single photon detectors

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Proceedings Article | 21 April 2000 Paper

Fluorescence spectroscopy of molecules on surfaces by indirect 2-photon excitation

Joerg Enderlein

Proceedings Volume 3922, (2000) https://doi.org/10.1117/12.383340

KEYWORDS: Molecules, Luminescence, Nonlinear optics, Fluorescence spectroscopy, Absorption, Scattering, Laser scattering, Raman scattering, Second-harmonic generation, Molecular spectroscopy

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Proceedings Article | 3 May 1999 Paper

Comparison between a conventional epifluorescence microscope and a new highly efficient evanescent wave detector in single-molecule spectroscopic applications

Joerg Enderlein, Thomas Ruckstuhl, Frank Loescher, Martin Boehmer, Stefan Seeger

Proceedings Volume 3602, (1999) https://doi.org/10.1117/12.347511

KEYWORDS: Molecules, Microscopes, Luminescence, Confocal microscopy, Objectives, Sensors, Glasses, Signal detection, Fluorescence spectroscopy, Molecular lasers

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Proceedings Article | 21 April 1999 Paper

Highly efficient optical detection of surface-generated fluorescence

Joerg Enderlein, Thomas Ruckstuhl, Stefan Seeger

Proceedings Volume 3603, (1999) https://doi.org/10.1117/12.346729

KEYWORDS: Luminescence, Molecules, Glasses, Fluorescence spectroscopy, Interfaces, Spherical lenses, Refractive index, Reflection, Imaging systems, Light scattering

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Proceedings Article | 15 May 1998 Paper

Probing the heterogeneous fluorescence-lifetime behavior of single rhodamine-6G molecules on fused silica

W. Patrick Ambrose, Peter Goodwin, Joerg Enderlein, David Semin, Richard Keller

Proceedings Volume 3270, (1998) https://doi.org/10.1117/12.308379

KEYWORDS: Molecules, Luminescence, Silica, Near field scanning optical microscopy, Rhodamine, Molecular lasers, Microscopy, Single photon, Near field, Optical properties

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Proceedings Article | 10 April 1998 Paper

Comparison between time-correlated single-photon counting and fluorescence correlation spectroscopy in single-molecule identification

Joerg Enderlein, Malte Koellner

Proceedings Volume 3259, (1998) https://doi.org/10.1117/12.307340

KEYWORDS: Molecules, Fluorescence correlation spectroscopy, Luminescence, Photons, Single photon, Diffusion, Fluorescence spectroscopy, Error analysis, Molecular spectroscopy, Information operations

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Proceedings Article | 22 May 1997 Paper

All-solid-state TCPC instrument for dynamic lifetime measurements in sensitive DNA analysis

Rainer Erdmann, Michael Wahl, Kristian Lauritsen, Joerg Enderlein, Benjamin Legendre, Daryl Williams, Steven Soper

Proceedings Volume 2985, (1997) https://doi.org/10.1117/12.274364

KEYWORDS: Picosecond phenomena, Semiconductor lasers, Luminescence, Single photon, Capillaries, Solid state electronics, Solid state lasers, Electronics, Light scattering, Electro optical modeling

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Proceedings Article | 7 May 1997 Paper

Modeling single-molecule detection statistics

Joerg Enderlein, David Robbins, W. Patrick Ambrose, Peter Goodwin, Richard Keller

Proceedings Volume 2980, (1997) https://doi.org/10.1117/12.273570

KEYWORDS: Molecules, Luminescence, Photons, Molecular lasers, Capillaries, Quantum efficiency, Statistical modeling, Absorption, Diffusion, Data modeling

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Proceedings Article | 1 April 1996 Paper

High-speed electronics for the detection of time-resolved fluorescence in a continuous flow system

Rainer Erdmann, Uwe Ortmann, Joerg Enderlein, Wolfgang Becker, Michael Wahl, Edgar Klose

Proceedings Volume 2680, (1996) https://doi.org/10.1117/12.237635

KEYWORDS: Molecules, Luminescence, Photons, Picosecond phenomena, High speed electronics, Fluorescence spectroscopy, Time resolved spectroscopy, Molecular lasers, Laser induced fluorescence, Clocks

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Proceedings Article | 1 April 1996 Paper

High-repetion-rate picosecond diode lasers for potential use in single-molecule detection

Uwe Ortmann, Rolf Krahl, Gerald Kell, Rainer Erdmann, Joerg Enderlein, Edgar Klose

Proceedings Volume 2680, (1996) https://doi.org/10.1117/12.237636

KEYWORDS: Semiconductor lasers, Molecules, Picosecond phenomena, Fluorescence spectroscopy, Molecular lasers, Laser systems engineering, Electronics, Liquids, Single photon, Lasers

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Proceedings Article | 8 May 1995 Paper

Electron transfer dyes as standard for picosecond fluorescence decay measurements

Jens Sobek, Arno Wiehe, Joerg Enderlein, Rainer Erdmann, Harry Kurreck

Proceedings Volume 2388, (1995) https://doi.org/10.1117/12.208495

KEYWORDS: Luminescence, Picosecond phenomena, Absorption, Zinc, Systems modeling, Antimony, Quantum efficiency, Electroluminescence, Catalysis, Oxidation

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Proceedings Article | 8 May 1995 Paper

Lifetime-based identification of single molecules

Joerg Enderlein, Rolf Krahl, Uwe Ortmann, Michael Wahl, Rainer Erdmann, Edgar Klose

Proceedings Volume 2388, (1995) https://doi.org/10.1117/12.208465

KEYWORDS: Molecules, Luminescence, Rhodamine, Photons, Bioalcohols, Molecular lasers, Time metrology, Signal detection, Electronics, Data processing

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Proceedings Article | 8 May 1995 Paper

Simultaneous detection of time-resolved emission spectra using a multianode PMT and new time-correlated single-photon counting (TCSPC) electronics with a 5-MHz count rate

Rainer Erdmann, Wolfgang Becker, Uwe Ortmann, Joerg Enderlein

Proceedings Volume 2388, (1995) https://doi.org/10.1117/12.208493

KEYWORDS: Picosecond phenomena, Sensors, Electronics, Luminescence, Single photon, Ultrafast phenomena, Monochromators, Time metrology, Oscilloscopes, Signal detection

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Proceedings Article | 22 December 1994 Paper

Very compact all solid state fluorescence lifetime measurement system: preliminary results

Rainer Erdmann, Gerald Kell, Rolf Krahl, Uwe Ortmann, Wolfgang Becker, Joerg Enderlein, Edgar Klose

Proceedings Volume 2328, (1994) https://doi.org/10.1117/12.197519

KEYWORDS: Picosecond phenomena, Luminescence, Sensors, Semiconductor lasers, Diodes, Electronics, Signal detection, Ultrafast phenomena, Single photon, Monochromators

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Proceedings Article | 21 July 1994 Paper

Ultrasensitive fluorescence detection and maximum likelihood method

Joerg Enderlein

Proceedings Volume 2136, (1994) https://doi.org/10.1117/12.180805

KEYWORDS: Molecules, Photons, Fluorescence spectroscopy, Luminescence, Molecular lasers, Data analysis, Signal to noise ratio, Diffusion, Error analysis, Lead

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Showing 5 of 45 publications

Proceedings Volume Editor (16)

SPIE Conference Volume | 21 May 2018

Single Molecule Spectroscopy and Superresolution Imaging XI

SPIE Conference Volume | 28 April 2017

Single Molecule Spectroscopy and Superresolution Imaging X

SPIE Conference Volume | 7 July 2016

Single Molecule Spectroscopy and Superresolution Imaging IX

SPIE Conference Volume | 14 April 2015

Single Molecule Spectroscopy and Superresolution Imaging VIII

SPIE Conference Volume | 1 April 2014

Single Molecule Spectroscopy and Superresolution Imaging VII

Showing 5 of 16 publications

Conference Committee Involvement (29)

Single Molecule Spectroscopy and Superresolution Imaging XVIII

25 January 2025 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XVII

27 January 2024 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XVI

28 January 2023 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XV

22 January 2022 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XIV

6 March 2021 | Online Only, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XIII

1 February 2020 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XII

2 February 2019 | San Francisco, California, United States

Nanoimaging and Nanospectroscopy VI

19 August 2018 | San Diego, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XI

27 January 2018 | San Francisco, California, United States

Nanoimaging and Nanospectroscopy V

6 August 2017 | San Diego, California, United States

Single Molecule Spectroscopy and Superresolution Imaging X

28 January 2017 | San Francisco, California, United States

Nanoimaging and Nanospectroscopy IV

28 August 2016 | San Diego, California, United States

Single Molecule Spectroscopy and Superresolution Imaging IX

13 February 2016 | San Francisco, California, United States

Nanoimaging and Nanospectroscopy III

9 August 2015 | San Diego, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VIII

7 February 2015 | San Francisco, California, United States

Nanoimaging and Nanospectroscopy II

17 August 2014 | San Diego, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VII

1 February 2014 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VI

2 February 2013 | San Francisco, California, United States

Nanoimaging and Nanospectroscopy

27 August 2012 | San Diego, California, United States

Single Molecule Spectroscopy and Superresolution Imaging V

21 January 2012 | San Francisco, California, United States

Single Molecule Spectroscopy and Imaging IV

22 January 2011 | San Francisco, California, United States

Single Molecule Spectroscopy and Imaging III

23 January 2010 | San Francisco, California, United States

Single Molecule Spectroscopy and Imaging II

24 January 2009 | San Jose, California, United States

Single Molecule Spectroscopy and Imaging

19 January 2008 | San Jose, California, United States

Ultrasensitive and Single-Molecule Detection Technologies II

20 January 2007 | San Jose, California, United States

Ultrasensitive and Single-Molecule Detection Technologies

21 January 2006 | San Jose, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II

24 January 2005 | San Jose, CA, United States

Imaging, Manipulation and Analysis of Biomolecules, Cells, and Tissues

27 January 2004 | San Jose, CA, United States

Manipulation and Analysis of Biomolecules, Cells and Tissues

28 January 2003 | San Jose, CA, United States

Showing 5 of 29 Conference Committees

Course Instructor

SC859: Single Molecule Spectroscopy and Imaging

This course introduces the basics of single molecule fluorescence spectroscopy and imaging under am-bient conditions especially for biologically relevant applications. The first part covers the technological requirements for single molecule fluorescence detection and spectroscopy, covering excitation sources, microscopy optics, optical and mechanical components, single photon detectors, fast photon-counting electronics, data acquisition, processing, visualization and evaluation. The second part focuses on several single-molecule spectroscopic and imaging techniques: fluores-cence-correlation spectroscopy (FCS) and its different variants such as 2-focus FCS, fluorescence life-time correlations spectroscopy (FLCS), fluorescence cross-correlation spectroscopy (FCCS), or photo-electron transfer mediated FCS (PET-FCS); fluorescence intensity distribution analysis (FIDA) alias photon-counting histogram analysis (PCH); single-molecule Förster resonance energy transfer (smFRET); focused and defocused single-molecule imaging and fluorescence lifetime imaging (FLIM). Selected applications will be presented: monitoring conformational changes in proteins, fast conforma-tional fluctuations in peptides, single-molecule motion and reorientation of polymer-embedded chro-mophores and of motor proteins, single molecule and single quantum dot photophysics, and super-resolution fluorescence microscopy.

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