Prof. Jörg Enderlein
Professor/Biophysical Chemistry at Georg-August-Univ Göttingen
SPIE Involvement:
Conference Program Committee | Conference Chair | Author | Editor | Instructor
Publications (54)

Proceedings Article | 4 March 2019
Proc. SPIE. 10884, Single Molecule Spectroscopy and Superresolution Imaging XII
KEYWORDS: Thin films, Plasmons, Super resolution, Graphene, Metals, Molecules, Energy transfer, Super resolution microscopy, Resonance energy transfer, Fluorescence resonance energy transfer

SPIE Conference Volume | 28 April 2017

Proceedings Article | 24 April 2017
Proc. SPIE. 10071, Single Molecule Spectroscopy and Superresolution Imaging X
KEYWORDS: Surface plasmons, Metals, Luminescence, Molecules, Energy transfer, Dielectrics, Distance measurement, Resonance energy transfer, Fluorescence resonance energy transfer, Molecular energy transfer

Proceedings Article | 24 April 2017
Proc. SPIE. 10071, Single Molecule Spectroscopy and Superresolution Imaging X
KEYWORDS: Confocal microscopy, Diffraction, Super resolution, 3D acquisition, Imaging systems, Stereoscopy, Microscopy, Image resolution, Live cell imaging, 3D image processing

Showing 5 of 54 publications
Conference Committee Involvement (24)
Single Molecule Spectroscopy and Superresolution Imaging XIII
1 February 2020 | San Francisco, California, United States
Single Molecule Spectroscopy and Superresolution Imaging XII
2 February 2019 | San Francisco, California, United States
Nanoimaging and Nanospectroscopy VI
19 August 2018 | San Diego, California, United States
Single Molecule Spectroscopy and Superresolution Imaging XI
27 January 2018 | San Francisco, California, United States
Nanoimaging and Nanospectroscopy V
6 August 2017 | San Diego, California, United States
Showing 5 of 24 Conference Committees
Course Instructor
SC859: Single Molecule Spectroscopy and Imaging
This course introduces the basics of single molecule fluorescence spectroscopy and imaging under am-bient conditions especially for biologically relevant applications. The first part covers the technological requirements for single molecule fluorescence detection and spectroscopy, covering excitation sources, microscopy optics, optical and mechanical components, single photon detectors, fast photon-counting electronics, data acquisition, processing, visualization and evaluation. The second part focuses on several single-molecule spectroscopic and imaging techniques: fluores-cence-correlation spectroscopy (FCS) and its different variants such as 2-focus FCS, fluorescence life-time correlations spectroscopy (FLCS), fluorescence cross-correlation spectroscopy (FCCS), or photo-electron transfer mediated FCS (PET-FCS); fluorescence intensity distribution analysis (FIDA) alias photon-counting histogram analysis (PCH); single-molecule Förster resonance energy transfer (smFRET); focused and defocused single-molecule imaging and fluorescence lifetime imaging (FLIM). Selected applications will be presented: monitoring conformational changes in proteins, fast conforma-tional fluctuations in peptides, single-molecule motion and reorientation of polymer-embedded chro-mophores and of motor proteins, single molecule and single quantum dot photophysics, and super-resolution fluorescence microscopy.
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