We investigate the use of first derivative intrinsic fluorescence spectroscopy as an adjunctive tool for early diagnosis or
screening on gynecological tumors. The first derivative intrinsic fluorescence spectra from limosis morning urine of
gynecologic cancerous patient and the healthy group are measured. And Combining the first order derivative spectra
method and clinic diagnosis standard to cluster analysis the information, we obtained that the diagnostic sensitivity and
specificity are 81% and 75% based on the judgment of setting γ=300, respectively. It may be applied to early diagnose
gynecological tumors using intrinsic urine fluorescence spectra.
Ovarian cancer has the highest mortality rate among the gynecologic cancers, and it goes undetected because adequate
technology does not exist to detect preinvasive or early stage disease. Fluorescence spectroscopy of urine may provide a
cost-effective tool to improve precancer detection. This study describes initial investigation of the potential of intrinsic
urine fluorescence spectra for detecting early ovarian cancer.
Using the Xenon arc lamp to irradiate the urine from ovarian cancer, cervical carcinoma groups and healthy donors, we
obtained fluorescence emission spectra. The three groups of samples show different emission spectra peak and
fluorescence intensity. Ovarian cancer group has the largest displacement of maximum spectra peak at 380-400nm
excitation wavelength. And the fluorescence intensity from ovarian cancer group is significantly higher with the healthy
donors and cervical carcinoma group in comparison at the short wave excitation region of 320-360nm, while cervical
carcinoma group has higher fluorescence intensity than ovarian cancer group at 380-500nm excitation. Characteristic
fluorescence mechanism was studied through comparison of fluorescence spectra and software fitting image.
Fluorescence spectra at 440nm excitation is found to be perfectly lorentzian fitted by three characteristic fluorescence
peaks, which are originated form coproporphyrin, riboflavin and p-hydroxyphenol derivatives existing in the urine.