Because of the ability of integrating the strengths of different modalities and providing fully integrated information,
multi-modality molecular imaging techniques provide an excellent solution to detecting and diagnosing earlier cancer,
which remains difficult to achieve by using the existing techniques. In this paper, we present an overview of our research
efforts on the development of the optical imaging-centric multi-modality molecular imaging platform, including the
development of the imaging system, reconstruction algorithms and preclinical biomedical applications. Primary
biomedical results show that the developed optical imaging-centric multi-modality molecular imaging platform may
provide great potential in the preclinical biomedical applications and future clinical translation.
Immunocytochemical and immunofluorescence staining are used for identifying the characteristics of metastasis in
traditional ways. Micro-computed tomography (micro-CT) is a useful tool for monitoring and longitudinal imaging of
tumor in small animal in vivo. In present study, we evaluated the feasibility of the detection for metastasis of gastric
carcinoma by high-resolution micro-CT system with omnipaque accumulative enhancement method in the organs.
Firstly, a high-resolution micro-CT ZKKS-MCT-sharp micro-CT was developed by our research group and Guangzhou
Zhongke Kaisheng Medical Technology Co., Ltd. Secondly, several gastric carcinoma models were established through
inoculating 2x10<sup>6</sup> BGC-823 gastric carcinoma cells subcutaneously. Thirdly, micro-CT scanning was performed after
accumulative enhancement method of intraperitoneal injection of omnipaque contrast agent containing 360 mg iodine
with a concentration of 350 mg I/ml. Finally, we obtained high-resolution anatomical information of the metastasis in
vivo in a BALB/c NuNu nude mouse, the 3D tumor architecture is revealed in exquisite detail at about 35 μm spatial
resolution. In addition, the accurate shape and volume of the micrometastasis as small as 0.78 mm<sup>3</sup> can be calculated
with our software. Overall, our data suggest that this imaging approach and system could be used to enhance the
understanding of tumor proliferation, metastasis and could be the basis for evaluating anti-tumor therapies.
An experimental cone-beam Micro-CT system for small animal imaging is presented in the paper. The system
is designed to obtain high-resolution anatomic information and will be integrated with our bioluminescence
tomography system. A flat panel X-ray detector (CMOS technology with a column CsI scintillator plate, 50
micron pixel size, 120 mm × 120 mm photodiode area) and a
micro-focus X-ray source (13 to 40 μm of focal spot
size) are used in the system. The object (mouse or rat) is placed on a three-degree (two translations and one
rotation) programming stage and could be located to an accurate position in front of the detector. The large field
of view (FOV) of the system allows us to acquire the whole body imaging of a normal mouse in one scanning
which usually takes about 6 to 15 minutes. Raw data from X-ray detector show spatial variation caused by
dark image offset, pixel gain and defective pixels, therefore data pre-processing is needed before reconstruction.
Geometry calibrations are also used to reduce the artifacts caused by geometric misalignment. In order to
accelerate FDK filtered backprojection method, we develop a reconstruction software using GPU hardware in
our system. System spacial resolution and image uniformity and voxel noise have been assessed and mouse
reconstruction images are illuminated in the paper. Experiment results show that this system is suitable for
small animal imaging.
Conference Committee Involvement (3)
Proceedings of World Molecular Imaging Congress 2011
7 September 2011 |
Proceedings of SPIE Symposium on Medical Imaging 2011
12 February 2011 |
Proceedings of World Molecular Imaging Congress 2010