A dynamic surface-plasmon-resonance (SPR) imaging sensor was developed to realize high-resolution high-throughput
applications. The SPR device consisted of a half-cylinder prism, 47.5nm-thick gold thin film and a custom-designed
flow cell to construct the Kretschmann configuration. A cylindrical lens pair in conjunction with the half-cylinder
prism was used to simplify the optical alignment procedure and to ensure plane-wave propagation inside the prism.
Phase-shifting interferometry was implemented by using a piezoelectric transducer (PZT) driven by a triangular voltage
waveform. A CCD camera was employed to acquire the sequential interference patterns required for phase calculations.
A reference signal obtained from a photodiode before the SPR device was used to compensate the system instability from
the laser intensity, environmental disturbances, and mechanical vibrations from the PZT. Integrating-bucket data
acquisition was realized with the synchronization between the photodiode and the CCD camera to preserve the dynamic
capability of the SPR sensor. System evaluations were performed by salt-water mixture measurements and gold-spot
array imaging. The achieved phase-measurement stability was 0.40 degrees and the system sensitivity was 5.14×10<sup>4</sup>
degree/RIU (refractive index unit). The corresponding system resolution was 7.8×10<sup>-6</sup> RIU. This SPR imager is
anticipated to find applications in studying biomolecular interactions with high resolution, stability, throughput and