The anterior nuclei of the thalamus (ANT) have been a promising target in order to control and reduce epileptic seizures for deep brain stimulation surgery (DBS). There are several theories on the structural connectivity of ANT, but clear evidence is still missing. The clinical studies show that each subdivision of the ANT presents different patterns of connectivity throughout the hippocampus, mammillary bodies, and neocortex. Diffusion MRI is a well-known technique that non‐invasively investigates the microstructural organization and orientation of biological tissues in vivo. Diffusion tensor imaging (DTI) is one of the models that has been widely accepted in order to examine the human brain, although it does not accurately reveal the fiber orientations of complex structures due to the presence of crossing fibers. Constrained spherical deconvolution (CSD) can be used to reveal the fiber orientations, overcoming the limitations of DTI. Recent studies show that the b value and gradient directions also play a significant role in extracting fiber orientations in such complex structures. These methods enable a more accurate tractography and investigation of the structural connectivity. In this paper, we demonstrate an approach for the connectivity analysis of ANT by determining different ROIs in the Papez circuit.