We demonstrate high spatial resolution imaging of a stromal cut in the ex-vivo pig
cornea, using second- and third-harmonic generation microscopy. From these images,
we see in detail how the cut affects the corneal layers. In the beginning of the cut, the
anterior layers, in which the blade is passing through, are disorganized, which could
explain the shadows observed on the images. In the stroma, the cut can be imaged by
third harmonic microscopy, probably due to the χ3 contrast. Although the current
results were obtained from the healthy ex-vivo cornea, it already allows one to
understand the effects of the cut on the tissue characteristics (such as scattering).