Wide field of view (FOV) retinal imaging with high resolution has been demonstrated for quantitative analysis of retinal
microstructures. An adaptive optics scanning laser ophthalmoscope (AO-SLO) that was built in our laboratory was
improved by a customized scanning protocol for scanning wide region. A post-processing program was developed for
generating wide FOV retinal images. The high resolution retinal image with 1.7 degree by 3.0 degree FOV were
Adaptive optics spectral domain optical coherence tomography (AO SD-OCT) has provided three-dimensional high isotropic
resolution retinal images <i>in vivo</i>. In order to enhance the image quality of deep region of the eye, the alternative wavelength
of 1-μm has been used for ophthalmic OCT. This study aims to develop AO SD-OCT with one-micrometer probe
and demonstrated high penetration and high resolution retinal imaging. A broadband 1-μm SLD light source (Suplerlum)
have the center wavelength of 1.03 μm and the spectral bandwidth of 106 nm. Axial scans were obtained by an InGaAs line
scan camera with the speed of 47,000 Hz. The aberrations of the system and the eye were measured by Shack-Hartmann
wavefront sensor (HASO32, Imagine Eyes, France) and corrected by a single deformable mirror (Mirao52, Imagine Eyes).
The AO closed loop was working with the iteration frequency of 7 Hz. The residual root mean square (RMS) wavefront
error was typically reduced to 0.1 μm. Seven eyes of 7 normal subjects were examined. The signal gain was found for all
subjects with AO. The waving interface of nerve fiber layer and ganglion cell layer, the interface between ganglion cell
layer and inner plexiform layer and choroid-sclera interface were observed. AO SD-OCT with one-micrometer probe may
be useful not only for the investigation of photoreceptors but also nerve fiber abnormalities.